Literature summary for 2.4.1.5 extracted from

Miller, A.W.; Robyt, J.F.
Inhibition of dextransucrase by Zn2+, Ni2+, Co2+, and Tris(hydroxymethyl)aminomethane (Tris) (1986), Arch. Biochem. Biophys., 248, 579-586.

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General Stability

General Stability	Organism
dextran T10 stabilizes	Leuconostoc mesenteroides

Inhibitors

Inhibitors	Comment	Organism	Structure
Ba2+	-	Leuconostoc mesenteroides
Co2+	inhibition by binding to 2 types of metal ion sites, one type consists of a single site and has a low apparent affinity to Ca2+, at the remaining site(s), Ca2+ has a much higher apparent affinity than Zn2+, Ni2+ or Co2+ and prevents inhibition by these metal ions	Leuconostoc mesenteroides
Mg2+	-	Leuconostoc mesenteroides
Mn2+	-	Leuconostoc mesenteroides
Ni2+	inhibition by binding to 2 types of metal ion sites, one type consists of a single site and has a low apparent affinity to Ca2+, at the remaining site(s), Ca2+ has a much higher apparent affinity than Zn2+, Ni2+ or Co2+ and prevents inhibition by these metal ions	Leuconostoc mesenteroides
Sr2+	-	Leuconostoc mesenteroides
Tris	competitive with sucrose	Leuconostoc mesenteroides
Zn2+	inhibition by binding to 2 types of metal ion sites, one type consists of a single site and has a low apparent affinity to Ca2+, at the remaining site(s), Ca2+ has a much higher apparent affinity than Zn2+, Ni2+ or Co2+ and prevents inhibition by these metal ions	Leuconostoc mesenteroides

Organism

Organism	UniProt	Comment	Textmining
Leuconostoc mesenteroides	-	NRRl B-512(F)	-
Leuconostoc mesenteroides NRRl B-512(F)	-	NRRl B-512(F)	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
sucrose + (1,6-alpha-D-glucosyl)n	-	Leuconostoc mesenteroides	D-fructose + (1,6-alpha-D-glucosyl)n+1	-	?
sucrose + (1,6-alpha-D-glucosyl)n	-	Leuconostoc mesenteroides NRRl B-512(F)	D-fructose + (1,6-alpha-D-glucosyl)n+1	-	?

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
4.2	-	Ni2+	-	Leuconostoc mesenteroides
12.2	-	Tris	-	Leuconostoc mesenteroides

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Release 2023.1 (January 2023)