Literature summary for 2.4.1.5 extracted from

Moulis, C.; Arcache, A.; Escalier, P.C.; Rinaudo, M.; Monsan, P.; Remaud-Simeon, M.; Potocki-Veronese, G.
High-level production and purification of a fully active recombinant dextransucrase from Leuconostoc mesenteroides NRRL B-512F (2006), FEMS Microbiol. Lett., 261, 203-210.

Search for more literature for 2.4.1.5

Cloned(Commentary)

Cloned (Comment)	Organism
DSR-S protein is fused to a thioredoxin tag at the N-terminal extremity, and a 6*His tag at the C-terminal end and expressed in Escherichia coli	Leuconostoc mesenteroides

Protein Variants

Protein Variants	Comment	Organism
additional information	rational deletions of the signal peptide, the beginning of the variable region and the last four repeats of the C-terminal end cause no loss of activity. The new variant successfully purified is remarkably stable. With a kcat of 584 per s, it is the most efficient recombinant glucansucrase described to date. The synthesized polymer possesses more than 95% of alpha-1,6 links, like the dextran produced by the native enzyme	Leuconostoc mesenteroides

Organism

Organism	UniProt	Comment	Textmining
Leuconostoc mesenteroides	-	NRRL B-512F	-
Leuconostoc mesenteroides NRRL B-512F	-	NRRL B-512F	-

Purification (Commentary)

Purification (Comment)	Organism
-	Leuconostoc mesenteroides

Synonyms

Synonyms	Comment	Organism
DSR-S	-	Leuconostoc mesenteroides

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Release 2023.1 (January 2023)