Cloned (Comment) | Organism |
---|---|
gene gtfR, expression of wild-type and mutant enzymes in Escherichia coli strain XL10-Gold | Streptococcus oralis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | random mutagenesis of the most conserved motif around the transition state stabilizer in glucansucrase GTFR of Streptococcus oralis, yielding different variants with altered reaction specificity, generation of a mutant gtfR library, overview | Streptococcus oralis |
N1134S | the mutation in GTFA results in a drastically changed specificity but no major changes in polymer versus oligosaccharide formation | Streptococcus oralis |
R624G | the R624G mutations near the transition state stabilizer is involved in the phenotype which exhibits a drastic switch in regioselectivity from a dextran type with mainly alpha-1,6-glucosidic linkages to a mutant type polymer with predominantly alpha-1,3-glucosidic linkages | Streptococcus oralis |
R624G/V630I | the mutant exhibits a drastic switch in regioselectivity from a dextran type with mainly alpha-1,6-glucosidic linkages to a mutant type polymer with predominantly alpha-1,3-glucosidic linkages, both mutations near the transition state stabilizer, R624G and V630I, are contributing to this alteration | Streptococcus oralis |
R624G/V630I/D717A | the mutant exhibits a drastic switch in regioselectivity from a dextran type with mainly alpha-1,6-glucosidic linkages to a mutant type polymer with predominantly alpha-1,3-glucosidic linkages, both mutations near the transition state stabilizer, R624G and V630I, are contributing to this alteration | Streptococcus oralis |
S628D | saturation mutagenesis, the mutation guides the reaction toward the synthesis of short chain oligosaccharides with a drastically increased yield of 47% isomaltose or 64% leucrose | Streptococcus oralis |
S628R | saturation mutagenesis, the mutation guides the reaction toward the synthesis of short chain oligosaccharides with a drastically increased yield of 47% isomaltose or 64% leucrose | Streptococcus oralis |
V630I | the V630I mutations near the transition state stabilizer is involved in the phenotype which exhibits a drastic switch in regioselectivity from a dextran type with mainly alpha-1,6-glucosidic linkages to a mutant type polymer with predominantly alpha-1,3-glucosidic linkages | Streptococcus oralis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
sucrose | pH 7.0, 30°C, mutant R624G | Streptococcus oralis | |
1.6 | - |
sucrose | pH 7.0, 30°C, mutant V630I | Streptococcus oralis | |
2 | - |
sucrose | pH 7.0, 30°C, wild-type enzyme | Streptococcus oralis | |
3.1 | - |
sucrose | pH 7.0, 30°C, mutant S628D | Streptococcus oralis | |
3.5 | - |
sucrose | pH 7.0, 30°C, mutant R624G/V630I | Streptococcus oralis | |
5 | - |
sucrose | pH 7.0, 30°C, mutant S628R | Streptococcus oralis | |
9.8 | - |
sucrose | pH 7.0, 30°C, mutant R624G/V630I/D717A | Streptococcus oralis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus oralis | - |
gene gtfR | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + acceptor | - |
Streptococcus oralis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glucansucrase | - |
Streptococcus oralis |
GTFR | - |
Streptococcus oralis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Streptococcus oralis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
sucrose | pH 7.0, 30°C, mutant S628R | Streptococcus oralis | |
5.2 | - |
sucrose | pH 7.0, 30°C, mutant R624G | Streptococcus oralis | |
14.5 | - |
sucrose | pH 7.0, 30°C, mutant R624G/V630I | Streptococcus oralis | |
22.4 | - |
sucrose | pH 7.0, 30°C, wild-type enzyme | Streptococcus oralis | |
25.6 | - |
sucrose | pH 7.0, 30°C, mutant R624G/V630I/D717A | Streptococcus oralis | |
29.8 | - |
sucrose | pH 7.0, 30°C, mutant S628D | Streptococcus oralis | |
32.6 | - |
sucrose | pH 7.0, 30°C, mutant V630I | Streptococcus oralis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Streptococcus oralis |