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Literature summary for 2.4.1.5 extracted from

  • Vettori, M.; Franchetti, S.; Contiero, J.
    Structural characterization of a new dextran with a low degree of branching produced by Leuconostoc mesenteroides FT045B dextransucrase (2012), Carbohydr. Polym., 88, 1440-1444.
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Leuconostoc mesenteroides
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sucrose + [(1->6)-alpha-D-glucosyl]n Leuconostoc mesenteroides
-
D-fructose + [(1->6)-alpha-D-glucosyl]n+1 the structure of the commercial B-512F dextran synthesized by strain B-512F dextran sucrase is composed of D-glucose residues, containing 95% alpha-(1,6) linkages in the main chains and 5% alpha-(1,3) branch linkages ?
sucrose + [(1->6)-alpha-D-glucosyl]n Leuconostoc mesenteroides
-
D-fructose + [(1->6)-alpha-D-glucosyl]n+1 the structure of the dextran synthesized by strain FT045B dextran sucrase is composed of D-glucose residues, containing 97.9% alpha-(1,6) linkages in the main chains and 2.1% alpha-(1,3) branch linkages ?
sucrose + [(1->6)-alpha-D-glucosyl]n Leuconostoc mesenteroides B-512FMC
-
D-fructose + [(1->6)-alpha-D-glucosyl]n+1 the structure of the commercial B-512F dextran synthesized by strain B-512F dextran sucrase is composed of D-glucose residues, containing 95% alpha-(1,6) linkages in the main chains and 5% alpha-(1,3) branch linkages ?
sucrose + [(1->6)-alpha-D-glucosyl]n Leuconostoc mesenteroides B-512FMC
-
D-fructose + [(1->6)-alpha-D-glucosyl]n+1 the structure of the dextran synthesized by strain FT045B dextran sucrase is composed of D-glucose residues, containing 97.9% alpha-(1,6) linkages in the main chains and 2.1% alpha-(1,3) branch linkages ?
sucrose + [(1->6)-alpha-D-glucosyl]n Leuconostoc mesenteroides FT045B
-
D-fructose + [(1->6)-alpha-D-glucosyl]n+1 the structure of the commercial B-512F dextran synthesized by strain B-512F dextran sucrase is composed of D-glucose residues, containing 95% alpha-(1,6) linkages in the main chains and 5% alpha-(1,3) branch linkages ?
sucrose + [(1->6)-alpha-D-glucosyl]n Leuconostoc mesenteroides FT045B
-
D-fructose + [(1->6)-alpha-D-glucosyl]n+1 the structure of the dextran synthesized by strain FT045B dextran sucrase is composed of D-glucose residues, containing 97.9% alpha-(1,6) linkages in the main chains and 2.1% alpha-(1,3) branch linkages ?

Organism

Organism UniProt Comment Textmining
Leuconostoc mesenteroides
-
a constitutive mutant of Leuconostoc mesenteroides B-512FMC
-
Leuconostoc mesenteroides
-
a constitutive mutant of Leuconostoc mesenteroides FT045B
-
Leuconostoc mesenteroides B-512FMC
-
a constitutive mutant of Leuconostoc mesenteroides B-512FMC
-
Leuconostoc mesenteroides FT045B
-
a constitutive mutant of Leuconostoc mesenteroides FT045B
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
21 U/ml, pH 5.2, 30°C Leuconostoc mesenteroides
additional information
-
89 U/ml, pH 5.2, 30°C Leuconostoc mesenteroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + [(1->6)-alpha-D-glucosyl]n
-
Leuconostoc mesenteroides D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
?
sucrose + [(1->6)-alpha-D-glucosyl]n
-
Leuconostoc mesenteroides D-fructose + [(1->6)-alpha-D-glucosyl]n+1 the structure of the commercial B-512F dextran synthesized by strain B-512F dextran sucrase is composed of D-glucose residues, containing 95% alpha-(1,6) linkages in the main chains and 5% alpha-(1,3) branch linkages ?
sucrose + [(1->6)-alpha-D-glucosyl]n
-
Leuconostoc mesenteroides D-fructose + [(1->6)-alpha-D-glucosyl]n+1 the structure of the dextran synthesized by strain FT045B dextran sucrase is composed of D-glucose residues, containing 97.9% alpha-(1,6) linkages in the main chains and 2.1% alpha-(1,3) branch linkages ?
sucrose + [(1->6)-alpha-D-glucosyl]n
-
Leuconostoc mesenteroides B-512FMC D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
?
sucrose + [(1->6)-alpha-D-glucosyl]n
-
Leuconostoc mesenteroides B-512FMC D-fructose + [(1->6)-alpha-D-glucosyl]n+1 the structure of the commercial B-512F dextran synthesized by strain B-512F dextran sucrase is composed of D-glucose residues, containing 95% alpha-(1,6) linkages in the main chains and 5% alpha-(1,3) branch linkages ?
sucrose + [(1->6)-alpha-D-glucosyl]n
-
Leuconostoc mesenteroides B-512FMC D-fructose + [(1->6)-alpha-D-glucosyl]n+1 the structure of the dextran synthesized by strain FT045B dextran sucrase is composed of D-glucose residues, containing 97.9% alpha-(1,6) linkages in the main chains and 2.1% alpha-(1,3) branch linkages ?
sucrose + [(1->6)-alpha-D-glucosyl]n
-
Leuconostoc mesenteroides FT045B D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
?
sucrose + [(1->6)-alpha-D-glucosyl]n
-
Leuconostoc mesenteroides FT045B D-fructose + [(1->6)-alpha-D-glucosyl]n+1 the structure of the commercial B-512F dextran synthesized by strain B-512F dextran sucrase is composed of D-glucose residues, containing 95% alpha-(1,6) linkages in the main chains and 5% alpha-(1,3) branch linkages ?
sucrose + [(1->6)-alpha-D-glucosyl]n
-
Leuconostoc mesenteroides FT045B D-fructose + [(1->6)-alpha-D-glucosyl]n+1 the structure of the dextran synthesized by strain FT045B dextran sucrase is composed of D-glucose residues, containing 97.9% alpha-(1,6) linkages in the main chains and 2.1% alpha-(1,3) branch linkages ?

Synonyms

Synonyms Comment Organism
B-512FMC dextransucrase
-
Leuconostoc mesenteroides
FT045B dextransucrase
-
Leuconostoc mesenteroides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Leuconostoc mesenteroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2
-
assay at Leuconostoc mesenteroides