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Literature summary for 2.4.1.5 extracted from
- Purification and characterization of an extracellular dextransucrase from Pediococcus pentosaceus isolated from the soil of North East India (2011), Food Technol. Biotechnol., 49, 297-303.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 20% inhibition at 1 mM | Pediococcus pentosaceus |  |
| additional information | no effect by 7 M urea on enzyme activity | Pediococcus pentosaceus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Pediococcus pentosaceus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | activates 150% at 6 mM | Pediococcus pentosaceus | |
| MgCl2 | activates 5% at 1 mM | Pediococcus pentosaceus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 180000 | - |
native PAGE | Pediococcus pentosaceus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose + [(1->6)-alpha-D-glucosyl]n | Pediococcus pentosaceus | - |
D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Acetone | at 50%, reduces the enzyme activity by 94% | Pediococcus pentosaceus |
| acetonitrile | at 20%, reduces the enzyme activity by 80% | Pediococcus pentosaceus |
| DMSO | at 90%, reduces the enzyme activity by 91% | Pediococcus pentosaceus |
| Ethanol | at 50%, reduces the enzyme activity by 80% | Pediococcus pentosaceus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pediococcus pentosaceus | - |
isolated from soil from a sugarcane field in Assam, India | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native extracellular enzyme by a single-step fractionation using polyethylene glycols of different molecular mass, 31fold purification by PEG 400 and 45fold purification by PEG 1500 | Pediococcus pentosaceus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.6 | - |
crude enzyme, culture supernatant, pH 5.4, 30°C | Pediococcus pentosaceus |
| 18 | - |
PEG 400-purified enzyme, pH 5.4, 30°C | Pediococcus pentosaceus |
| 36 | - |
PEG 1500-purified enzyme, pH 5.4, 30°C | Pediococcus pentosaceus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | optimization of conditions for dextransucrase activity assay | Pediococcus pentosaceus | ? | - |
? | |
| sucrose + [(1->6)-alpha-D-glucosyl]n | - |
Pediococcus pentosaceus | D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Pediococcus pentosaceus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.4 | - |
- |
Pediococcus pentosaceus |
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