Application | Comment | Organism |
---|---|---|
synthesis | purified Weissella confusa Cab3 dextransucrase (WcCab3-DSR) is used for in vitro synthesis of dextran and glucooligosaccharides | Weissella confusa |
General Stability | Organism |
---|---|
0.1% glycerol and 1.0% Tween 80 enhance enzyme stability, enzyme half-life at 30°C increases from 10 h to 74 h and 59 h, respectively, stabilization by 0.01 mg/ml PEG-8000 | Weissella confusa |
dextran T-500 slightly stabilizes | Weissella confusa |
glutaraldehyde slightly destabilizes | Weissella confusa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 98% inhibition at 5 mM | Weissella confusa | |
Mn2+ | 42% inhibition at 10 mM | Weissella confusa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates by 20% at 1 mM | Weissella confusa | |
Co2+ | activates by 21% at 1 mM | Weissella confusa | |
additional information | poor effects by Zn2+, Mg2+, and Ni2+ at 1-2 mM | Weissella confusa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Weissella confusa | A0A0F6TP16 | - |
- |
Purification (Comment) | Organism |
---|---|
dextransucrase WcCab3-DSR 11fold by PEG fractionation | Weissella confusa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.9 | - |
purified enzyme, pH 5.4, 35°C | Weissella confusa |
Storage Stability | Organism |
---|---|
-20°C, purified enzyme, half-life: 37.9 days | Weissella confusa |
-4°C, purified enzyme, half-life: 69.4 days | Weissella confusa |
30°C, purified enzyme, half-life: 10.33 h | Weissella confusa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Cab3 dextransucrase WcCab3-DSR is used for in vitro synthesis of dextran and glucooligosaccharides, product analysis by NMR and and mass spectrometry. Glucooligosaccarides are produced through the acceptor reaction with maltose | Weissella confusa | ? | - |
? | |
sucrose + maltose | - |
Weissella confusa | D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | glucooligosaccarides are produced through the acceptor reaction with maltose, a homologous series of isomaltooligosac-charides with reducing end maltose units | ? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | substrate is dextran | Weissella confusa | D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | the produced dextran confirmes the presence of main chain alpha-(1->6) linkages with only 3.0% of alpha-(1->3) branching, of which some are elongated | ? |
Synonyms | Comment | Organism |
---|---|---|
Cab3 | - |
Weissella confusa |
WcCab3-DSR | - |
Weissella confusa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Weissella confusa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
glycerol and Tween 80 enhance enzyme stability, enzyme half-life at 30°C increases from 10 h to 74 h and 59 h, respectively | Weissella confusa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.4 | - |
- |
Weissella confusa |