Literature summary for 2.4.1.5 extracted from

Ruehmkorf, C.; Bork, C.; Mischnick, P.; Ruebsam, H.; Becker, T.; Vogel, R.F.
Identification of Lactobacillus curvatus TMW 1.624 dextransucrase and comparative characterization with Lactobacillus reuteri TMW 1.106 and Lactobacillus animalis TMW 1.971 dextransucrases (2013), Food Microbiol., 34, 52-61.

Search for more literature for 2.4.1.5

Activating Compound

Activating Compound	Comment	Organism	Structure
exopolysaccharides	stimulate, whereby concentrations of 0.5% cause the strongest effects of up to 1.3fold	Limosilactobacillus reuteri
exopolysaccharides	stimulate, whereby concentrations of 0.5% cause the strongest effects of up to 1.5-2fold	Latilactobacillus curvatus
fructan	activates the enzyme	Ligilactobacillus animalis
Glucan	-	Latilactobacillus curvatus
Glucan	-	Limosilactobacillus reuteri
Glucan	activates the enzyme	Ligilactobacillus animalis
additional information	the enzyme is strongly activated through its own exopolysaccharide by 1.5-2fold. The fructan of Lactobacillus sanfranciscensis has either no effect or an inhibitory one	Ligilactobacillus animalis

Application

Application	Comment	Organism
food industry	the exopolysaccharides of Lactobacillus animalis TMW 1.971 improve the quality of gluten-free breads, they can be produced in situ to levels enabling baking applications	Ligilactobacillus animalis
food industry	the exopolysaccharides of Lactobacillus curvatus TMW 1.624 improve the quality of gluten-free breads, they can be produced in situ to levels enabling baking applications	Latilactobacillus curvatus
food industry	the exopolysaccharides of Lactobacillus reuteri TMW 1.106 improve the quality of gluten-free breads, they can be produced in situ to levels enabling baking applications	Limosilactobacillus reuteri

Cloned(Commentary)

Cloned (Comment)	Organism
gene gtf, recombinant expression in Escherichia coli strain Rosetta	Latilactobacillus curvatus
gene gtf, recombinant expression in Escherichia coli strain Rosetta	Limosilactobacillus reuteri
gene gtf, recombinant expression in Escherichia coli strain Rosetta	Ligilactobacillus animalis

Inhibitors

Inhibitors	Comment	Organism	Structure
Cu2+	-	Latilactobacillus curvatus
Cu2+	-	Ligilactobacillus animalis
Cu2+	-	Limosilactobacillus reuteri
EDTA	-	Latilactobacillus curvatus
EDTA	-	Ligilactobacillus animalis
fructan	the fructan of Lactobacillus sanfranciscensis has either no effect or an inhibitory effect	Ligilactobacillus animalis
Mn2+	inhibits hydrolysis activity almost entirely, but activates transferase activity at 1 mM	Limosilactobacillus reuteri
Zn2+	inhibits hydrolysis activity but has no effect on transferase activity which constitutes to more than 90% of the overall activity, Zn2+ has therefore no influence on overall activity	Latilactobacillus curvatus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Ligilactobacillus animalis	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	activates at 1 mM, best activating metal ion	Latilactobacillus curvatus
Ca2+	activates at 1 mM, best activating metal ion	Limosilactobacillus reuteri
Ca2+	activates at 1 mM, best activating metal ion	Ligilactobacillus animalis
K+	activates at 1 mM	Latilactobacillus curvatus
K+	activates at 1 mM	Ligilactobacillus animalis
Mg2+	activates at 1 mM	Ligilactobacillus animalis
Mn2+	activates at 1 mM	Latilactobacillus curvatus
Mn2+	inhibits hydrolysis activity almost entirely, but activates transferase activity at 1 mM	Limosilactobacillus reuteri
additional information	Mn2+ has no influence on hydrolysis and transferase activity. Al3+ and Na+ also do not significantly influence overall activity	Ligilactobacillus animalis
additional information	no effect by 1 mM EDTA	Limosilactobacillus reuteri
Na+	activates at 1 mM	Latilactobacillus curvatus
Zn2+	activates at 1 mM	Limosilactobacillus reuteri
Zn2+	activates at 1 mM	Ligilactobacillus animalis
Zn2+	inhibits hydrolysis activity but has no effect on transferase activity which constitutes to more than 90% of the overall activity, Zn2+ has therefore no influence on overall activity. Al3+ and Mg2+ also have no significant influence on activity	Latilactobacillus curvatus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
183630	-	x * 183630, sequence calculation	Latilactobacillus curvatus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
sucrose + [(1->6)-alpha-D-glucosyl]n	Latilactobacillus curvatus	-	D-fructose + [(1->6)-alpha-D-glucosyl]n+1	-	?
sucrose + [(1->6)-alpha-D-glucosyl]n	Limosilactobacillus reuteri	-	D-fructose + [(1->6)-alpha-D-glucosyl]n+1	-	?
sucrose + [(1->6)-alpha-D-glucosyl]n	Ligilactobacillus animalis	-	D-fructose + [(1->6)-alpha-D-glucosyl]n+1	-	?
sucrose + [(1->6)-alpha-D-glucosyl]n	Latilactobacillus curvatus TMW 1.624	-	D-fructose + [(1->6)-alpha-D-glucosyl]n+1	-	?
sucrose + [(1->6)-alpha-D-glucosyl]n	Limosilactobacillus reuteri TMW 1.106	-	D-fructose + [(1->6)-alpha-D-glucosyl]n+1	-	?
sucrose + [(1->6)-alpha-D-glucosyl]n	Ligilactobacillus animalis TMW 1.971	-	D-fructose + [(1->6)-alpha-D-glucosyl]n+1	-	?

Organism

Organism	UniProt	Comment	Textmining
Latilactobacillus curvatus	-	gene gtf	-
Latilactobacillus curvatus TMW 1.624	-	gene gtf	-
Ligilactobacillus animalis	-	gene gtf	-
Ligilactobacillus animalis TMW 1.971	-	gene gtf	-
Limosilactobacillus reuteri	-	gene gtf	-
Limosilactobacillus reuteri TMW 1.106	-	gene gtf	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	growth and exopolysaccharide production correlated strongly for strain TMW 1.106, as soon as the strains reaches the stationary phase, exopolysaccharide production decreases and stops shortly afterward	Limosilactobacillus reuteri	-
additional information	growth and exopolysaccharide production correlated strongly for strain TMW 1.624, as soon as the strains reaches the stationary phase, exopolysaccharide production decreases and stops shortly afterward	Latilactobacillus curvatus	-
additional information	growth and exopolysaccharide production correlated strongly for strain TMW 1.971, as soon as the strains reaches the stationary phase, exopolysaccharide production decreases and stops shortly afterward	Ligilactobacillus animalis	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
sucrose + [(1->6)-alpha-D-glucosyl]n	-	Latilactobacillus curvatus	D-fructose + [(1->6)-alpha-D-glucosyl]n+1	-	?
sucrose + [(1->6)-alpha-D-glucosyl]n	-	Limosilactobacillus reuteri	D-fructose + [(1->6)-alpha-D-glucosyl]n+1	-	?
sucrose + [(1->6)-alpha-D-glucosyl]n	-	Ligilactobacillus animalis	D-fructose + [(1->6)-alpha-D-glucosyl]n+1	-	?
sucrose + [(1->6)-alpha-D-glucosyl]n	-	Latilactobacillus curvatus	D-fructose + [(1->6)-alpha-D-glucosyl]n+1	structural properties of exopolysaccharides produced, overview	?
sucrose + [(1->6)-alpha-D-glucosyl]n	-	Limosilactobacillus reuteri	D-fructose + [(1->6)-alpha-D-glucosyl]n+1	structural properties of exopolysaccharides produced, overview	?
sucrose + [(1->6)-alpha-D-glucosyl]n	-	Ligilactobacillus animalis	D-fructose + [(1->6)-alpha-D-glucosyl]n+1	structural properties of exopolysaccharides produced, overview	?
sucrose + [(1->6)-alpha-D-glucosyl]n	-	Latilactobacillus curvatus TMW 1.624	D-fructose + [(1->6)-alpha-D-glucosyl]n+1	-	?
sucrose + [(1->6)-alpha-D-glucosyl]n	-	Latilactobacillus curvatus TMW 1.624	D-fructose + [(1->6)-alpha-D-glucosyl]n+1	structural properties of exopolysaccharides produced, overview	?
sucrose + [(1->6)-alpha-D-glucosyl]n	-	Limosilactobacillus reuteri TMW 1.106	D-fructose + [(1->6)-alpha-D-glucosyl]n+1	-	?
sucrose + [(1->6)-alpha-D-glucosyl]n	-	Limosilactobacillus reuteri TMW 1.106	D-fructose + [(1->6)-alpha-D-glucosyl]n+1	structural properties of exopolysaccharides produced, overview	?
sucrose + [(1->6)-alpha-D-glucosyl]n	-	Ligilactobacillus animalis TMW 1.971	D-fructose + [(1->6)-alpha-D-glucosyl]n+1	-	?
sucrose + [(1->6)-alpha-D-glucosyl]n	-	Ligilactobacillus animalis TMW 1.971	D-fructose + [(1->6)-alpha-D-glucosyl]n+1	structural properties of exopolysaccharides produced, overview	?

Subunits

Subunits	Comment	Organism
?	x * 183630, sequence calculation	Latilactobacillus curvatus
More	the enzyme contains conserved and less-conserved YG-repeats	Latilactobacillus curvatus

Synonyms

Synonyms	Comment	Organism
Gtf	-	Latilactobacillus curvatus
Gtf	-	Limosilactobacillus reuteri
Gtf	-	Ligilactobacillus animalis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
31	-	-	Latilactobacillus curvatus
42	-	transferase activity, two temperature maxima at 42°C and 53°C for overall activity	Ligilactobacillus animalis
45	-	-	Limosilactobacillus reuteri
53	-	hydrolysis activity, two temperature maxima at 42°C and 53°C for overall activity	Ligilactobacillus animalis

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
15	38	activity range, inactive at 40°C, profile overview	Latilactobacillus curvatus
22	59	more than 50% of maximal activity over the broad temperature profile	Ligilactobacillus animalis
25	53	activity range, profile overview	Limosilactobacillus reuteri

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4.4	-	-	Latilactobacillus curvatus
4.4	-	-	Limosilactobacillus reuteri
5.8	-	-	Ligilactobacillus animalis

pH Range

pH Minimum	pH Maximum	Comment	Organism
4.8	7.2	broad range with activities higher than 60% of maximal activity	Ligilactobacillus animalis

General Information

General Information	Comment	Organism
evolution	sequence comparisons of the four conserved regions I, II, III and IV in the catalytic domains of different Lactobacillus dextransucrases	Latilactobacillus curvatus
evolution	sequence comparisons of the four conserved regions I, II, III and IV in the catalytic domains of different Lactobacillus dextransucrases	Limosilactobacillus reuteri
evolution	sequence comparisons of the four conserved regions I, II, III and IV in the catalytic domains of different Lactobacillus dextransucrases	Ligilactobacillus animalis
metabolism	growth and exopolysaccharide production correlated strongly for strain TMW 1.106	Limosilactobacillus reuteri
metabolism	growth and exopolysaccharide production correlated strongly for strain TMW 1.624	Latilactobacillus curvatus
metabolism	growth and exopolysaccharide production correlated strongly for strain TMW 1.971	Ligilactobacillus animalis
additional information	lactate is formed during production of exopolysaccharides	Latilactobacillus curvatus
additional information	lactate is formed during production of exopolysaccharides	Limosilactobacillus reuteri
additional information	Lactobacillus animalis is a strong acidifier. The pH decreases fast to pH 3.75 and after 30 h fermentation, 167.14 mM lactate is formed during production of exopolysaccharides	Ligilactobacillus animalis

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Release 2023.1 (January 2023)