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Literature summary for 2.4.1.66 extracted from
- Collagen glucosyl- and galactosyltransferases of cultured human fetal lung fibroblasts (1981), Arch. Biochem. Biophys., 210, 678-690.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | enzyme activity is dependent on aging of IMR-90 cells | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0021 | - |
calf skin collagen | - |
Homo sapiens | |
| 0.026 | - |
D-galactosylhydroxylysine | - |
Homo sapiens |  |
| 0.048 | - |
UDP-glucose | calf skin collagen as acceptor | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 10 mM, effectively substitutes for Mn2+ | Homo sapiens | |
| Mg2+ | 38% as effective as Mn2+ | Homo sapiens | |
| Mn2+ | requirement | Homo sapiens | |
| Mn2+ | 5 mM | Homo sapiens | |
| additional information | not activated by Co2+ | Homo sapiens | |
| additional information | not activated by Ni2+ | Homo sapiens | |
| additional information | not activated by Cd2+ | Homo sapiens | |
| additional information | not activated by Fe2+ | Homo sapiens | |
| additional information | not activated by Zn2+, Cu2+ | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | Homo sapiens | posttranslational modification of collagen | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | Homo sapiens | together with EC 2.4.1.50 involved in biosynthesis of glucosylgalactosylhydroxylysine units in collagens, basement membranes and certain serum glycoproteins | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Homo sapiens |
| solubilized with Triton X-100 | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fibroblast | fetal lung WI-38 and IMR-90 diploid fibroblasts, in cell suspension culture | Homo sapiens | - |
| IMR-90 cell | fetal lung WI-38 and IMR-90 diploid fibroblasts, in cell suspension culture | Homo sapiens | - |
| WI-38 cell | fetal lung WI-38 and IMR-90 diploid fibroblasts, in cell suspension culture | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | not: glucose free bovine glomerular basement membranes, calf thyroglobulin, galactose | Homo sapiens | ? | - |
? | |
| UDP-alpha-D-glucose + calf skin collagen | - |
Homo sapiens | ? | - |
? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | calf skin collagen | Homo sapiens | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | product is glucosylgalactosylhydroxylysine-collagen | ? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | calf skin gelatin | Homo sapiens | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | product is glucosylgalactosylhydroxylysine-collagen | ? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | glucosyl-acceptors: ichthyocol, ichthyocol glycopeptides obtained by collagenase digestion, bovine glomerular basement membrane | Homo sapiens | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | product is glucosylgalactosylhydroxylysine-collagen | ? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | posttranslational modification of collagen | Homo sapiens | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | together with EC 2.4.1.50 involved in biosynthesis of glucosylgalactosylhydroxylysine units in collagens, basement membranes and certain serum glycoproteins | Homo sapiens | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
| UDP-glucose + D-galactosylhydroxylysine | good substrate | Homo sapiens | UDP + 1,2-D-glucosyl-D-galactosylhydroxylysine | - |
? | |
| UDP-glucose + D-galactosylhydroxylysine | in vitro | Homo sapiens | UDP + 1,2-D-glucosyl-D-galactosylhydroxylysine | - |
? | |
| UDP-glucose + ichthyocol | fish collagen prepared from carp swim bladder, ichthyocol glycopeptides prepared by collagenase digestion are better substrates than native ichthyocol, better substrate than calf skin collagen | Homo sapiens | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 6.5 | 2 pH-optima: 6-6.5 and 7.5-8, regardless of size of acceptor | Homo sapiens |
| 7.5 | 8 | 2 pH-optima: 6-6.5 and 7.5-8, regardless of size of acceptor | Homo sapiens |
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