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Literature summary for 2.4.1.66 extracted from
- Purification and properties of UDP-glucose galactosylhydroxylysine collagen glucosyltransferase (EC 2.4.1.?) from bovine arterial tissue (1975), Hoppe-Seyler's Z. Physiol. Chem., 356, 921-928.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.167 | - |
Collagen | denatured citrate-soluble calf skin collagen | Bos taurus |  |
| 0.28 | - |
Collagen | acetic acid-soluble collagen from alkali-treated calf arterial tissue | Bos taurus | |
| 0.33 | - |
Collagen | beta12-component of calf skin collagen | Bos taurus | |
| 0.63 | - |
Collagen | alpha-chain of calf skin collagen | Bos taurus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 50-60% as effective as Mn2+ | Bos taurus | |
| Mg2+ | 50-60% as effective as Mn2+ | Bos taurus | |
| Mn2+ | requirement | Bos taurus | |
| Mn2+ | 10 mM | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 72000 | - |
gel filtration | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | Bos taurus | glycosylation in the course of procollagen and collagen biosynthesis, carbohydrate attachment is an essential step in the protocollagen biosynthesis, postribosomal modification | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
calf | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 50fold | Bos taurus |
| partial | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| aorta thoracica | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 0.1 M Tris-buffer, pH 7.2, 0.2 M NaCl, 1 week, 10% loss of activity | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose + collagen | - |
Bos taurus | ? | - |
? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | requires UDP-glucose as glucosyl donor and acceptor protein with terminal galactose residues | Bos taurus | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | acetic acid-soluble collagen from alkali-treated calf arterial tissue | Bos taurus | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | denatured citrate-soluble calf skin collagen is the best substrate, its alpha1-chain and beta12-component are also acceptors, but with lower affinity | Bos taurus | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | glycosylation in the course of procollagen and collagen biosynthesis, carbohydrate attachment is an essential step in the protocollagen biosynthesis, postribosomal modification | Bos taurus | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
| UDP-glucose + asialo alpha 1-glycoprotein | sialic acid-free alpha1-acid glycoprotein of human serum, 1/50 of affinity of citrate-soluble calf skin collagen | Bos taurus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bos taurus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 58 | - |
denaturation at | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 4.2 and 8.1 | Bos taurus |
| 8.3 | - |
2 pH-optima: 8.3 and 9.9 | Bos taurus |
| 9.9 | - |
2 pH-optima: 8.3 and 9.9 | Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 11.5 | about half-maximal activity at pH 4.0 and 11.5 | Bos taurus |
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