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Literature summary for 2.4.1.66 extracted from

  • Wang, C.; Luosujärvi, H.; Heikkinen, J.; Risteli, M.; Uitto, L.; Myllylä, R.
    The third activity for lysyl hydroxylase 3: galactosylation of hydroxylysyl residues in collagens in vitro (2002), Matrix Biol., 21, 559-566.
    View publication on PubMed
Search for more literature for 2.4.1.66

Cloned(Commentary)

Cloned (Comment) Organism
a single gene codes for LH, GGT and GT activities Homo sapiens
LH cDNA with GGT activity is cloned and expressed in Escherichia coli BL21 (DE3) pLysS, a single gene codes for LH, GGT and GT activities Caenorhabditis elegans
LH3 cDNA with GGT activity is cloned and expressed in Sf9 insect cells and in Escherichia coli XL1-Blue Homo sapiens

Protein Variants

Protein Variants Comment Organism
C144I mutant with dramatically reduced GGT activity Homo sapiens
additional information mutation of aspartates in the positions 187-191 significantly reduces GGT activity of LH3 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
 UDP-glucose recombinant LH3/GGT Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Caenorhabditis elegans
-
-
soluble LH3/GGT protein Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
81000
-
 x * 81000, LH3 with GGT activity, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen Homo sapiens posttranslational modification of collagen UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen
-
 ?
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen Caenorhabditis elegans posttranslational modification of collagen UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen
-
 ?

Organism

Organism UniProt Comment Textmining
Caenorhabditis elegans
-
-
-
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-tagged recombinant LH3/GGT/GT protein expressed in Escherichia coli Homo sapiens
partial Homo sapiens
partial, His-tagged recombinant LH/GGT/GT protein expressed in Escherichia coli Caenorhabditis elegans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens
0.33
-
 GGT activity of LH3 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the amino acids important for the GGT activity are located in the N-terminal part of LH3/GGT protein separate from the C-terminal LH active site Homo sapiens ?
-
 ?
additional information multifunctional lysyl hydroxylase isoform 3, LH3 protein, possesses lysyl hydroxylase, collagen glucosyltransferase and galactosyltransferase activities Homo sapiens ?
-
 ?
additional information multifunctional lysyl hydroxylase possesses lysyl hydroxylase, collagen glucosyltransferase and galactosyltransferase activities Caenorhabditis elegans ?
-
 ?
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen calf skin gelatin Homo sapiens UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen product is glucosylgalactosylhydroxylysine-collagen ?
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen calf skin gelatin Caenorhabditis elegans UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen product is glucosylgalactosylhydroxylysine-collagen ?
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen posttranslational modification of collagen Homo sapiens UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen
-
 ?
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen posttranslational modification of collagen Caenorhabditis elegans UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen
-
 ?

Subunits

Subunits Comment Organism
? x * 81000, LH3 with GGT activity, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
More GGT activity is associated with lysyl hydroxylase isoform 3, LH3, EC 1.14.11.4 Homo sapiens
More GGT activity is associated with lysyl hydroxylase, LH Caenorhabditis elegans
More cf. EC 2.4.1.50 Homo sapiens
More cf. EC 2.4.1.50 Caenorhabditis elegans