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Literature summary for 2.4.1.7 extracted from
- Purification and mechanism of action of sucrose phosphorylase (1967), J. Biol. Chem., 242, 1338-1346.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | 100000 | gel filtration | Pelomonas saccharophila |
| 84000 | - |
sedimetation equilibrium centrifugation | Pelomonas saccharophila |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose + phosphate | Pelomonas saccharophila | double displacement mechanism | alpha-D-glucose 1-phosphate + D-fructose | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pelomonas saccharophila | - |
ATCC 9114 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| less than 10% impurities | Pelomonas saccharophila |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| sucrose + phosphate = D-fructose + alpha-D-glucose 1-phosphate | double displacement mechanism | Pelomonas saccharophila |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-D-glucose 1-phosphate + D-xylulose | - |
Pelomonas saccharophila | alpha-D-glucopyranosyl-D-xylulofuranoside + phosphate | - |
r | |
| alpha-D-glucose 1-phosphate + H2O | - |
Pelomonas saccharophila | alpha-D-glucose + phosphate | - |
ir | |
| alpha-D-glucose 1-phosphate + L-arabinose | - |
Pelomonas saccharophila | ? | - |
r | |
| alpha-D-glucose 1-phosphate + L-arabinulose | - |
Pelomonas saccharophila | ? | - |
r | |
| alpha-D-glucose-1-phosphate + L-sorbose | - |
Pelomonas saccharophila | alpha-D-glucosyl-alpha-L-sorbose + phosphate | - |
r | |
| glucose-1-phosphate + arsenate | - |
Pelomonas saccharophila | glucose-1-arsenate + phosphate | glucose-1-arsenate is further hydrolyzed to form glucose and arsenate | ir | |
| additional information | sucrose and alpha-glucose-1-phosphate are hydrolyzed in absence of phosphate and arsenate at very low rate | Pelomonas saccharophila | ? | - |
? | |
| sucrose + phosphate | double displacement mechanism | Pelomonas saccharophila | alpha-D-glucose 1-phosphate + D-fructose | - |
? | |
| sucrose + phosphate | double displacement mechanism | Pelomonas saccharophila | alpha-D-glucose 1-phosphate + D-fructose | - |
r | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | - |
hydrolysis | Pelomonas saccharophila |
| 7 | - |
phosphorylysis | Pelomonas saccharophila |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9 | - |
Pelomonas saccharophila |
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Release 2023.1 (January 2023)
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