Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.7 extracted from

  • Goedl, C.; Schwarz, A.; Minani, A.; Nidetzky, B.
    Recombinant sucrose phosphorylase from Leuconostoc mesenteroides: characterization, kinetic studies of transglucosylation, and application of immobilised enzyme for production of alpha-D-glucose 1-phosphate (2007), J. Biotechnol., 129, 77-86.
    View publication on PubMed

Application

Application Comment Organism
synthesis LmSPase is immobilised onto Eupergit C and used for the continuous production of alpha-D-glucose 1-phosphate from sucrose Leuconostoc mesenteroides

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, genetic structure, 60fold overexpression of LmSPase containing an 11 amino acid-long N-terminal metal affinity fusion peptide, with the sequence Arg-Gly-Ser-His6-Gly-Ser, in Escherichia coli DH10B Leuconostoc mesenteroides
expression of His-tagged enzyme in Escherichia coli DH10B Leuconostoc mesenteroides

Protein Variants

Protein Variants Comment Organism
additional information immobilization of the purified recombinant tagged enzyme for continuous production of alpha-D-glucose 1-phosphate from sucrose and phosphate in a packed bed reactor, method optimization, overview Leuconostoc mesenteroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Leuconostoc mesenteroides
5.7
-
sucrose pH 7.0, 30°C, recombinant enzyme Leuconostoc mesenteroides
9.5
-
phosphate pH 7.0, 30°C, recombinant enzyme Leuconostoc mesenteroides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
x * 55000 Leuconostoc mesenteroides
60000
-
x * 60000, SDS-PAGE Leuconostoc mesenteroides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sucrose + phosphate Leuconostoc mesenteroides
-
D-fructose + alpha-D-glucose 1-phosphate
-
r

Organism

Organism UniProt Comment Textmining
Leuconostoc mesenteroides
-
-
-
Leuconostoc mesenteroides Q59495
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Leuconostoc mesenteroides
recombinant LmSPase containing an 11 amino acid-long N-terminal metal affinity fusion peptide from Escherichia coli DH10B 7fold to homogeneity by metal affinity and hydrophobic interaction chromatography Leuconostoc mesenteroides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
190
-
-
Leuconostoc mesenteroides
190
-
purified recombinant tagged enzyme Leuconostoc mesenteroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-glucose 1-phosphate + D-arabitol transglucosylation Leuconostoc mesenteroides ?
-
?
alpha-D-glucose 1-phosphate + D-arabitol soluble recombinant enzyme Leuconostoc mesenteroides phosphate + alpha-D-glucosyl-D-arabitol
-
?
alpha-D-glucose 1-phosphate + L-arabitol soluble recombinant enzyme Leuconostoc mesenteroides phosphate + alpha-D-glucosyl-L-arabitol
-
?
alpha-L-glucose 1-phosphate + D-arabitol transglucosylation Leuconostoc mesenteroides ?
-
?
sucrose + phosphate
-
Leuconostoc mesenteroides D-fructose + alpha-D-glucose 1-phosphate
-
?
sucrose + phosphate
-
Leuconostoc mesenteroides D-fructose + alpha-D-glucose 1-phosphate
-
r

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE Leuconostoc mesenteroides
? x * 55000 Leuconostoc mesenteroides

Synonyms

Synonyms Comment Organism
LmSPase
-
Leuconostoc mesenteroides
SPase
-
Leuconostoc mesenteroides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Leuconostoc mesenteroides

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
22 45
-
Leuconostoc mesenteroides

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
maximal stability Leuconostoc mesenteroides
30
-
purified recombinant enzyme, stable below Leuconostoc mesenteroides

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
96
-
alpha-D-glucose 1-phosphate pH 7.0, 30°C, synthesis, recombinant enzyme Leuconostoc mesenteroides
165
-
sucrose pH 7.0, 30°C, phosphorolysis, recombinant enzyme Leuconostoc mesenteroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7 both reaction directions Leuconostoc mesenteroides

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7.5
-
Leuconostoc mesenteroides

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 7.5 maximal stability Leuconostoc mesenteroides
7 7.5 purified recombinant enzyme, completely stable Leuconostoc mesenteroides