Application | Comment | Organism |
---|---|---|
synthesis | LmSPase is immobilised onto Eupergit C and used for the continuous production of alpha-D-glucose 1-phosphate from sucrose | Leuconostoc mesenteroides |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, genetic structure, 60fold overexpression of LmSPase containing an 11 amino acid-long N-terminal metal affinity fusion peptide, with the sequence Arg-Gly-Ser-His6-Gly-Ser, in Escherichia coli DH10B | Leuconostoc mesenteroides |
expression of His-tagged enzyme in Escherichia coli DH10B | Leuconostoc mesenteroides |
Protein Variants | Comment | Organism |
---|---|---|
additional information | immobilization of the purified recombinant tagged enzyme for continuous production of alpha-D-glucose 1-phosphate from sucrose and phosphate in a packed bed reactor, method optimization, overview | Leuconostoc mesenteroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Leuconostoc mesenteroides | |
5.7 | - |
sucrose | pH 7.0, 30°C, recombinant enzyme | Leuconostoc mesenteroides | |
9.5 | - |
phosphate | pH 7.0, 30°C, recombinant enzyme | Leuconostoc mesenteroides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
x * 55000 | Leuconostoc mesenteroides |
60000 | - |
x * 60000, SDS-PAGE | Leuconostoc mesenteroides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + phosphate | Leuconostoc mesenteroides | - |
D-fructose + alpha-D-glucose 1-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc mesenteroides | - |
- |
- |
Leuconostoc mesenteroides | Q59495 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Leuconostoc mesenteroides |
recombinant LmSPase containing an 11 amino acid-long N-terminal metal affinity fusion peptide from Escherichia coli DH10B 7fold to homogeneity by metal affinity and hydrophobic interaction chromatography | Leuconostoc mesenteroides |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
190 | - |
- |
Leuconostoc mesenteroides |
190 | - |
purified recombinant tagged enzyme | Leuconostoc mesenteroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-glucose 1-phosphate + D-arabitol | transglucosylation | Leuconostoc mesenteroides | ? | - |
? | |
alpha-D-glucose 1-phosphate + D-arabitol | soluble recombinant enzyme | Leuconostoc mesenteroides | phosphate + alpha-D-glucosyl-D-arabitol | - |
? | |
alpha-D-glucose 1-phosphate + L-arabitol | soluble recombinant enzyme | Leuconostoc mesenteroides | phosphate + alpha-D-glucosyl-L-arabitol | - |
? | |
alpha-L-glucose 1-phosphate + D-arabitol | transglucosylation | Leuconostoc mesenteroides | ? | - |
? | |
sucrose + phosphate | - |
Leuconostoc mesenteroides | D-fructose + alpha-D-glucose 1-phosphate | - |
? | |
sucrose + phosphate | - |
Leuconostoc mesenteroides | D-fructose + alpha-D-glucose 1-phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, SDS-PAGE | Leuconostoc mesenteroides |
? | x * 55000 | Leuconostoc mesenteroides |
Synonyms | Comment | Organism |
---|---|---|
LmSPase | - |
Leuconostoc mesenteroides |
SPase | - |
Leuconostoc mesenteroides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Leuconostoc mesenteroides |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | 45 | - |
Leuconostoc mesenteroides |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
maximal stability | Leuconostoc mesenteroides |
30 | - |
purified recombinant enzyme, stable below | Leuconostoc mesenteroides |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
96 | - |
alpha-D-glucose 1-phosphate | pH 7.0, 30°C, synthesis, recombinant enzyme | Leuconostoc mesenteroides | |
165 | - |
sucrose | pH 7.0, 30°C, phosphorolysis, recombinant enzyme | Leuconostoc mesenteroides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | both reaction directions | Leuconostoc mesenteroides |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.5 | - |
Leuconostoc mesenteroides |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | maximal stability | Leuconostoc mesenteroides |
7 | 7.5 | purified recombinant enzyme, completely stable | Leuconostoc mesenteroides |