Cloned (Comment) | Organism |
---|---|
expression in Escherihia coli strain DH10B | Leuconostoc mesenteroides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + phosphate | Leuconostoc mesenteroides | - |
D-fructose + alpha-D-glucose 1-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc mesenteroides | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
sucrose + phosphate = D-fructose + alpha-D-glucose 1-phosphate | reaction mechanism of transglucosylation, overview. Formation of 2-O- and 4-O-glycosidic isomers of alpha-D-glucopyranosyl glucose suggests that catalytic glucosyl transfer by the phosphorylase involves two different binding modes for the D-glucose acceptor | Leuconostoc mesenteroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | glucobioses, maltose, i.e. 4-O-alpha-D-glucopyranosyl glucose, and kojibiose, i.e. 2-O-alpha-D-glucopyranosyl glucose, are formed in large amounts by glucosyl transfer to glucose, exceeding in almost all cases the amount of the desired transfer product from 1,2-propandiol compounds, process optimization, overview. Formation of 2-O- and 4-O-glycosidic isomers of alpha-D-glucopyranosyl glucose suggests that catalytic glucosyl transfer by the phosphorylase involves two different binding modes for the D-glucose acceptor, structure-activity relationships, overview | Leuconostoc mesenteroides | ? | - |
? | |
sucrose + 1,2-propanediol | regioselective glucosylation | Leuconostoc mesenteroides | D-fructose + 2-O-(alpha-D-glucopyranosyl)-1,2-propanediol | - |
? | |
sucrose + 3-(3-methoxyphenoxy)-1,2-propanediol | regioselective glucosylation | Leuconostoc mesenteroides | D-fructose + 3-(3-methoxyphenoxy)-2-O-(alpha-D-glucopyranosyl)-1,2-propanediol | - |
? | |
sucrose + 3-allyloxy-1,2-propanediol | regioselective glucosylation | Leuconostoc mesenteroides | D-fructose + 3-allyloxy-2-O-(alpha-D-glucopyranosyl)-1,2-propanediol | - |
? | |
sucrose + 3-ethoxy-1,2-propanediol | regioselective glucosylation | Leuconostoc mesenteroides | D-fructose + 3-ethoxy-2-O-(alpha-D-glucopyranosyl)-1,2-propanediol | product distribution resulting from conversion of sucrose in the presence of 3-ethoxy-1,2-propanediol, overview | ? | |
sucrose + 3-methoxy-1,2-propanediol | regioselective glucosylation | Leuconostoc mesenteroides | D-fructose + 3-methoxy-2-O-(alpha-D-glucopyranosyl)-1,2-propanediol | - |
? | |
sucrose + 3-tert-butoxy-1,2-propanediol | regioselective glucosylation | Leuconostoc mesenteroides | D-fructose + 3-tert-butoxy-2-O-(alpha-D-glucopyranosyl)-1,2-propanediol | - |
? | |
sucrose + phosphate | - |
Leuconostoc mesenteroides | D-fructose + alpha-D-glucose 1-phosphate | - |
? | |
sucrose + phosphate | regioselective glucosylation | Leuconostoc mesenteroides | D-fructose + alpha-D-glucose 1-phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Leuconostoc mesenteroides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Leuconostoc mesenteroides |