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Literature summary for 2.4.1.8 extracted from
- Purification and properties of maltose phosphorylase from Lactobacillus brevis (1973), Agric. Biol. Chem., 37, 2813-2819.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CuSO4 | - |
Levilactobacillus brevis |  |
| HgCl2 | - |
Levilactobacillus brevis | |
| additional information | no inhibition by MgCl2, MnSO4, CaCl2, Pb(CH3COO)2, Ba(OH)2, ZnSO4, glucose 6-phosphate, glucose 1-phosphate, fructose 6-phosphate, AMP, EDTA, IAA | Levilactobacillus brevis | |
| p-chloromercuribenzoate | - |
Levilactobacillus brevis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.7 | - |
arsenate | - |
Levilactobacillus brevis | |
| 1.9 | - |
maltose | - |
Levilactobacillus brevis | |
| 2.6 | - |
phosphate | - |
Levilactobacillus brevis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
2 * 80000, SDS-PAGE | Levilactobacillus brevis |
| 150000 | - |
gel filtration | Levilactobacillus brevis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Levilactobacillus brevis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Levilactobacillus brevis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 18 | - |
- |
Levilactobacillus brevis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, several months without loss of activity | Levilactobacillus brevis |
| 2°C, several months | Levilactobacillus brevis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltose + arsenate | - |
Levilactobacillus brevis | D-glucose + D-glucose + arsenate | - |
? | |
| maltose + phosphate | - |
Levilactobacillus brevis | beta-D-glucose 1-phosphate + D-glucose | - |
r | |
| additional information | no substrate: maltitol, maltotriitol, sucrose, lactose, maltobiontic acid, maltotriose, maltotetraose | Levilactobacillus brevis | ? | - |
? | |
| additional information | no substrate: trehalose, isomaltose | Levilactobacillus brevis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 80000, SDS-PAGE | Levilactobacillus brevis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Levilactobacillus brevis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
30 min stable | Levilactobacillus brevis |
| 42 | - |
t1/2: 30 min | Levilactobacillus brevis |
| 46 | - |
t1/2: 10 min | Levilactobacillus brevis |
| 50 | - |
and above, inactivation | Levilactobacillus brevis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.4 | - |
- |
Levilactobacillus brevis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 7.2 | about half-maximal activity at pH 4.5 and pH 7.2 | Levilactobacillus brevis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.4 | 7.2 | stable between | Levilactobacillus brevis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no pyridoxal 5'-phosphate required | Levilactobacillus brevis |
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