Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli | Paenibacillus sp. |
Protein Variants | Comment | Organism |
---|---|---|
E487A | mutant loses both the phosphorolytic and the synthetic activity | Paenibacillus sp. |
E487Q | mutant loses the phosphorolytic activity, but retains the synthetic activity (13% of the activity of wild-type MapA) | Paenibacillus sp. |
S634V | mutant loses both the phosphorolytic and the synthetic activity | Paenibacillus sp. |
S635V | mutant loses both the phosphorolytic and the synthetic activity | Paenibacillus sp. |
Y351F | mutant loses both the phosphorolytic and the synthetic activity | Paenibacillus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | - |
Paenibacillus sp. | |
Cu2+ | 1 mM, 50°C, 30 min, abolishes the phosphorolytic activity almost completely | Paenibacillus sp. | |
diethyldicarbonate | - |
Paenibacillus sp. | |
Hg2+ | 1 mM, 50°C, 30 min, abolishes the phosphorolytic activity almost completely | Paenibacillus sp. | |
additional information | iodoacetoamide, EDTA, and phenylmethanesulfonyl fluoride have no inhibitory effect | Paenibacillus sp. | |
N-bromosuccinimide | 0.1 mM, 47% inhibition | Paenibacillus sp. | |
N-ethylmaleimide | - |
Paenibacillus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0036 | - |
beta-D-glucose 1-phosphate | 50°C, pH 7.0, mutant enzyme E487Q | Paenibacillus sp. | |
0.004 | - |
maltose | 50°C, pH 7.0, wild-type enzyme | Paenibacillus sp. | |
0.0043 | - |
D-glucose | 50°C, pH 7.0, mutant enzyme E487Q | Paenibacillus sp. | |
0.0067 | - |
beta-D-glucose 1-phosphate | 50°C, pH 7.0, wild-type enzyme | Paenibacillus sp. | |
0.0071 | - |
phosphate | 50°C, pH 7.0, wild-type enzyme | Paenibacillus sp. | |
0.0099 | - |
D-glucose | 50°C, pH 7.0, wild-type enzyme | Paenibacillus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe3+ | 1 mM, 50°C, 30 min, enhances the activity moderately | Paenibacillus sp. | |
Mn2+ | 1 mM, 50°C, 30 min, enhances the activity moderately | Paenibacillus sp. | |
Pb2+ | 1 mM, 50°C, 30 min, enhances the activity moderately | Paenibacillus sp. | |
Sn2+ | 1 mM, 50°C, 30 min, enhances the activity moderately | Paenibacillus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
87748 | - |
2 * 87748, calculated from sequence | Paenibacillus sp. |
90000 | - |
2 * 90000, SDS-PAGE | Paenibacillus sp. |
180000 | - |
gel filtration | Paenibacillus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus sp. | Q50LH0 | - |
- |
Paenibacillus sp. SH-55 | Q50LH0 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Paenibacillus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Paenibacillus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltose + phosphate | - |
Paenibacillus sp. | D-glucose + beta-D-glucose 1-phosphate | - |
r | |
maltose + phosphate | - |
Paenibacillus sp. SH-55 | D-glucose + beta-D-glucose 1-phosphate | - |
r | |
additional information | no activity with: isomaltose, trehalose, neotrehalose, sucrose, lactose, and cellobiose | Paenibacillus sp. | ? | - |
? | |
additional information | no activity with: isomaltose, trehalose, neotrehalose, sucrose, lactose, and cellobiose | Paenibacillus sp. SH-55 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 90000, SDS-PAGE | Paenibacillus sp. |
dimer | 2 * 87748, calculated from sequence | Paenibacillus sp. |
Synonyms | Comment | Organism |
---|---|---|
mpA | - |
Paenibacillus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
glycosynthesis | Paenibacillus sp. |
50 | - |
phosphorolysis | Paenibacillus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
15 min, pH 7.0, stable up to | Paenibacillus sp. |
55 | - |
15 min, pH 7.0, 70% of the original activity is retained | Paenibacillus sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.5 | - |
beta-D-glucose 1-phosphate | 50°C, pH 7.0, mutant enzyme E487Q | Paenibacillus sp. | |
3.8 | - |
D-glucose | 50°C, pH 7.0, mutant enzyme E487Q | Paenibacillus sp. | |
12.9 | - |
phosphate | 50°C, pH 7.0, wild-type enzyme | Paenibacillus sp. | |
17.3 | - |
maltose | 50°C, pH 7.0, wild-type enzyme | Paenibacillus sp. | |
43.5 | - |
beta-D-glucose 1-phosphate | 50°C, pH 7.0, wild-type enzyme | Paenibacillus sp. | |
49.4 | - |
D-glucose | 50°C, pH 7.0, wild-type enzyme | Paenibacillus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
glycosynthesis | Paenibacillus sp. |
7 | 7.5 | phosphorolysis | Paenibacillus sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | more than 50% of maximal activity, phosphorolysis | Paenibacillus sp. |
6 | 7 | more than 50% of maximal activity, glycosynthesis | Paenibacillus sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6 | 15 min, 50°C, most stable in the pH-range 5.5-6.0 | Paenibacillus sp. |
5.5 | 7 | 15 min, 50°C, retains 50% of the original activity in the pH-range 5.5-7.0 | Paenibacillus sp. |