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Literature summary for 2.4.1.8 extracted from
- Enzymatic synthesis of alpha-anomer-selective D-glucosides using maltose phosphorylase (2007), Biosci. Biotechnol. Biochem., 71, 1598-1600.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | enzymatic synthesis of alpha-anomer-selective D-glucosides using maltose phosphorylase | Enterococcus hirae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | enzymatic synthesis of alpha-anomer-selective D-glucosides using maltose phosphorylase | Enterococcus hirae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltose + phosphate | Enterococcus hirae | - |
D-glucose + beta-D-glucose 1-phosphate | - |
r |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enterococcus hirae | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| maltose + phosphate = D-glucose + beta-D-glucose 1-phosphate | the forward reaction proceeds via bi bi mechanism, the reverse reaction shows broad acceptor specificity and transfers the D-glucosyl moiety to verious sugars | Enterococcus hirae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Enterococcus hirae | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltose + phosphate | - |
Enterococcus hirae | D-glucose + beta-D-glucose 1-phosphate | - |
r | |
| maltose + phosphate | the enzyme transfers the D-glucose moiety to salicylic alcohol, acting as acceptor, to give salicyl-O-alpha-D-glucopyranoside, TLC product analysis, overview | Enterococcus hirae | D-glucose + beta-D-glucose 1-phosphate | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MPase | - |
Enterococcus hirae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
assay at | Enterococcus hirae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
assay at | Enterococcus hirae |
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Release 2023.1 (January 2023)
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