Any feedback?
Literature summary for 2.4.1.8 extracted from
- Increasing free-energy (ATP) conservation in maltose-grown Saccharomyces cerevisiae by expression of a heterologous maltose phosphorylase (2011), Metab. Eng., 13, 518-526.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of the enzyme in a Saccharomyces cerevisiae knockout strain showing no maltose-transport activity and a very low residual maltase activity, co-expression with the MAL11 maltose-transporter gene and Lactococcus lactis beta-phosphoglucomutase | Fructilactobacillus sanfranciscensis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | Replacing maltose hydrolysis with phosphorolysis, by co-expression of the maltose phosphorylase with MAL11 maltose-transporter gene and Lactococcus lactis beta-phosphoglucomutase, in a Saccharomyces cerevisiae straain IMK289, a maltose transporter and maltase knockout strain, increases the anaerobic biomass yield on maltose in anaerobic maltose-limited chemostat cultures by 26%, thus demonstrating the potential of phosphorolysis to improve the free-energy conservation of disaccharide metabolism in industrial microorganisms, phenotype, overview | Fructilactobacillus sanfranciscensis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltose + phosphate | Fructilactobacillus sanfranciscensis | - |
D-glucose + beta-D-glucose 1-phosphate | - |
r |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fructilactobacillus sanfranciscensis | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltose + phosphate | - |
Fructilactobacillus sanfranciscensis | D-glucose + beta-D-glucose 1-phosphate | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Fructilactobacillus sanfranciscensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | - |
assay at | Fructilactobacillus sanfranciscensis |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | maltose phosphorylase is a disaccharide phosphorylase | Fructilactobacillus sanfranciscensis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
