Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cycloserine | induction | Canis lupus familiaris | |
NAD+ | stimulation, best at 2 mM | Homo sapiens | |
NADH | stimulation | Homo sapiens | |
NADP+ | stimulation | Homo sapiens | |
NADPH | stimulation | Homo sapiens |
Application | Comment | Organism |
---|---|---|
medicine | potential therapeutic target for inherited sphingolipidoses such as Gaucher, Fabry, and Tay-Sachs disease | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
cloning and transient expression in GM-95 cell line, mutant cell line containing no enzyme activity, derived from melanoma mutant cell line B16, DNA sequence determination and analysis | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CDP-glucose | slight inhibition | Homo sapiens | |
Cu2+ | - |
Homo sapiens | |
D-threo-1-phenyl-2-(decanoylamino)-3-morpholino-1-propanol-HCl | in vivo depletion of enzyme and accumulation of ceramide; not DL-diastereomer | Canis lupus familiaris | |
D-threo-1-phenyl-2-(decanoylamino)-3-morpholino-1-propanol-HCl | - |
Homo sapiens | |
Fe2+ | - |
Homo sapiens | |
Fe3+ | - |
Homo sapiens | |
additional information | induction of enzyme by inhibitors in presence of higher ceramide levels | Canis lupus familiaris | |
additional information | induction of enzyme by inhibitors in presence of higher ceramide levels; no inhibition by conduritol B epoxide | Homo sapiens | |
N-butyldeoxynojirimycin | - |
Canis lupus familiaris | |
N-butyldeoxynojirimycin | nonspecific inhibitor | Homo sapiens | |
Tris | 58% loss of activity at 0.2 M | Homo sapiens | |
Zn2+ | - |
Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stimulates | Homo sapiens | |
Mg2+ | stimulates | Homo sapiens | |
Mn2+ | stimulates | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
448530 | - |
x * 448530, amino acid sequence determination | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + N-acylsphingosine | Canis lupus familiaris | - |
UDP + D-glucosyl-N-acylsphingosine | - |
? | |
UDP-glucose + N-acylsphingosine | Homo sapiens | enzyme is involved in renal growth, neuronal differentiation, the establishment of the water permeability barrier in keratinocytes, and multidrug resistance in cancer cell | UDP + D-glucosyl-N-acylsphingosine | - |
? | |
UDP-glucose + N-acylsphingosine | Homo sapiens | enzyme is involved in renal growth, neuronal differentiation, the establishment of the water permeability barrier in keratinocytes, and multidrug resistance in cancer cell | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Canis lupus familiaris | - |
MDCK cells | - |
Homo sapiens | - |
- |
- |
Homo sapiens | Q16739 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Homo sapiens | - |
keratinocyte | - |
Homo sapiens | - |
kidney | - |
Homo sapiens | - |
MDCK cell | - |
Canis lupus familiaris | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | octanoyl dihydrosphingosine, decanoyl sphingosine, and stearoyl sphingosine are poor substrates | Homo sapiens | ? | - |
? | |
UDP-glucose + N-acylsphingosine | - |
Canis lupus familiaris | UDP + D-glucosyl-N-acylsphingosine | - |
? | |
UDP-glucose + N-acylsphingosine | acceptor substrate specificity, overview | Homo sapiens | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
UDP-glucose + N-acylsphingosine | i.e. ceramide | Homo sapiens | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
UDP-glucose + N-acylsphingosine | i.e. ceramide | Canis lupus familiaris | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
UDP-glucose + N-acylsphingosine | overview: short-chain ceramide substrates | Homo sapiens | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
UDP-glucose + N-acylsphingosine | enzyme is involved in renal growth, neuronal differentiation, the establishment of the water permeability barrier in keratinocytes, and multidrug resistance in cancer cell | Homo sapiens | UDP + D-glucosyl-N-acylsphingosine | - |
? | |
UDP-glucose + N-acylsphingosine | enzyme is involved in renal growth, neuronal differentiation, the establishment of the water permeability barrier in keratinocytes, and multidrug resistance in cancer cell | Homo sapiens | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
UDP-glucose + N-octanoyl sphingosine | best substrate | Homo sapiens | UDP + D-glucosyl-N-octanoyl sphingosine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | - |
Homo sapiens |
? | x * 448530, amino acid sequence determination | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
GCS | - |
Homo sapiens |
GCS | - |
Canis lupus familiaris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Homo sapiens |