Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.80 extracted from

  • Marks, D.L.; Paul, P.; Kamisaka, Y.; Pagano, R.E.
    Methods for studying glucosylceramide synthase (2000), Methods Enzymol., 311, 50-59.
    View publication on PubMed

Application

Application Comment Organism
medicine
-
Homo sapiens
medicine enzyme inhibition is a possible target for chemotherapeutic agents for a number of diseases, including cancer Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
cloning from rat brain genetic library and expression in Escherichia coli BL21(DE3) Rattus norvegicus
functional expression in Escherichia coli BL21(DE3) Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
EDTA no inhibition Rattus norvegicus
additional information
-
Gallus gallus
additional information
-
Homo sapiens
additional information no inhibition by DTT Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi membrane integral membrane protein Sus scrofa 139
-
Golgi membrane orientation to the cytosolic side Rattus norvegicus 139
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ stimulates Mus musculus
Ca2+ stimulates Homo sapiens
Ca2+ stimulates Rattus norvegicus
Mg2+ stimulates Mus musculus
Mg2+ stimulates Homo sapiens
Mg2+ stimulates Rattus norvegicus
Mg2+ stimulates Sus scrofa
Mn2+ stimulates Mus musculus
Mn2+ stimulates Homo sapiens
Mn2+ stimulates Rattus norvegicus
Mn2+ stimulates Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
-
Mus musculus
additional information
-
-
Homo sapiens
additional information
-
-
Rattus norvegicus
additional information
-
enzyme forms dimeric or oligomeric complexes with another protein in the Golgi membrane vivo, topology Homo sapiens
additional information
-
enzyme forms dimeric or oligomeric complexes with another protein in the Golgi membrane vivo, topology Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucose + N-acylsphingosine Gallus gallus
-
UDP + D-glucosyl-N-acylsphingosine
-
?
UDP-glucose + N-acylsphingosine Homo sapiens
-
UDP + D-glucosyl-N-acylsphingosine
-
?
UDP-glucose + N-acylsphingosine Sus scrofa
-
UDP + D-glucosyl-N-acylsphingosine
-
?
UDP-glucose + N-acylsphingosine Mus musculus
-
UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
UDP-glucose + N-acylsphingosine Rattus norvegicus
-
UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
UDP-glucose + N-acylsphingosine Homo sapiens
-
UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
UDP-glucose + N-acylsphingosine Rattus norvegicus Sprague-Dawley
-
UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-
Homo sapiens
-
-
-
Homo sapiens Q16739
-
-
Mus musculus
-
-
-
Rattus norvegicus
-
Sprague-Dawley
-
Rattus norvegicus Q9R0E0
-
-
Rattus norvegicus Sprague-Dawley
-
Sprague-Dawley
-
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial from Golgi membranes Rattus norvegicus
solubilization method Rattus norvegicus
solubilization method Sus scrofa

Renatured (Commentary)

Renatured (Comment) Organism
reconstitution of solubilized enzyme in detergent-free lipid vesicles Rattus norvegicus
reconstitution of solubilized enzyme in detergent-free lipid vesicles Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
keratinocyte
-
Homo sapiens
-
liver
-
Mus musculus
-
liver
-
Homo sapiens
-
liver low level Rattus norvegicus
-
lung
-
Rattus norvegicus
-
lung
-
Homo sapiens
-
spleen
-
Rattus norvegicus
-
testis
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Gallus gallus
additional information
-
-
Mus musculus
additional information
-
-
Homo sapiens
additional information
-
-
Sus scrofa
additional information
-
fluorescence assay method Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucose + N-acylsphingosine
-
Gallus gallus UDP + D-glucosyl-N-acylsphingosine
-
?
UDP-glucose + N-acylsphingosine
-
Homo sapiens UDP + D-glucosyl-N-acylsphingosine
-
?
UDP-glucose + N-acylsphingosine
-
Sus scrofa UDP + D-glucosyl-N-acylsphingosine
-
?
UDP-glucose + N-acylsphingosine
-
Mus musculus UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
UDP-glucose + N-acylsphingosine
-
Rattus norvegicus UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
UDP-glucose + N-acylsphingosine
-
Homo sapiens UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
UDP-glucose + N-acylsphingosine i.e. ceramide Gallus gallus UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
UDP-glucose + N-acylsphingosine i.e. ceramide Mus musculus UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
UDP-glucose + N-acylsphingosine i.e. ceramide Homo sapiens UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
UDP-glucose + N-acylsphingosine i.e. ceramide Rattus norvegicus UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
UDP-glucose + N-acylsphingosine i.e. ceramide Sus scrofa UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
UDP-glucose + N-acylsphingosine
-
Rattus norvegicus Sprague-Dawley UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
UDP-glucose + N-acylsphingosine i.e. ceramide Rattus norvegicus Sprague-Dawley UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
UDP-glucose + N-[7-(4-nitrobenzo-2-oxa-1,3-diazole)]-6-aminocaproyl-D-erythro-sphingosine synthetic fluorescent ceramide substrate analogue for fluorescence enzyme assay Rattus norvegicus UDP + D-glucosyl-N-[7-(4-nitrobenzo-2-oxa-1,3-diazole)]-6-aminocaproyl-D-erythro-sphingosine
-
?
UDP-glucose + N-[7-(4-nitrobenzo-2-oxa-1,3-diazole)]-6-aminocaproyl-D-erythro-sphingosine synthetic fluorescent ceramide substrate analogue for fluorescence enzyme assay Rattus norvegicus Sprague-Dawley UDP + D-glucosyl-N-[7-(4-nitrobenzo-2-oxa-1,3-diazole)]-6-aminocaproyl-D-erythro-sphingosine
-
?

Synonyms

Synonyms Comment Organism
GCS
-
Mus musculus
GCS
-
Homo sapiens
GCS
-
Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Mus musculus
37
-
assay at Rattus norvegicus
37
-
assay at Sus scrofa
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
Mus musculus
7.4
-
-
Homo sapiens
7.4
-
assay at Rattus norvegicus