Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutants in Escherichia coli BL21(DE3) | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
C143A | site-directed mutagenesis, increased activity, reduced expression level compared to wild-type | Rattus norvegicus |
C207A | site-directed mutagenesis, reduced activity, reduced inhibitory effect of N-ethylmaleimide, reduced expression level compared to wild-type | Rattus norvegicus |
C213A | site-directed mutagenesis, reduced activity, reduced expression level compared to wild-type | Rattus norvegicus |
C232A | site-directed mutagenesis, reduced expresssion level compared to wild-type | Rattus norvegicus |
C290A | site-directed mutagenesis, reduced expression level compared to wild-type | Rattus norvegicus |
C296A | site-directed mutagenesis, reduced activity, reduced expression level compared to wild-type | Rattus norvegicus |
C321A | site-directed mutagenesis, reduced activity | Rattus norvegicus |
C321A/C323A | site-directed mutagenesis, reduced activity | Rattus norvegicus |
C343A | site-directed mutagenesis, enhanced activity, slightly reduced expression level compared to wild-type | Rattus norvegicus |
C384A | site-directed mutagenesis, enhanced activity | Rattus norvegicus |
C86A | site-directed mutagenesis, reduced activity, reduced expression level compared to wild-type | Rattus norvegicus |
C98A | site-directed mutagenesis, reduced activity, reduced expression level compared to wild-type | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-ethylmaleimide | attacks Cys207 | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
amino acid sequence of conserved D1,D2,D3,QXXRW motif | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | Q9R0E0 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-alpha-D-glucose + an N-acylsphingosine = UDP + a beta-D-glucosyl-N-acylsphingosine | UDP-glucose and inhibitor D-PDMP binding region overlap and contain His193 | Rattus norvegicus | |
UDP-alpha-D-glucose + an N-acylsphingosine = UDP + a beta-D-glucosyl-N-acylsphingosine | enzyme DNA sequence contains conserved D1,D2,D3,QXXRW motif found also in other progressive beta-glycosyltransferases, putative active site, Cys207 and His193 involved | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + N-acylsphingosine | i.e. ceramide | Rattus norvegicus | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
UDP-glucose + N-[7-(4-nitrobenzo-2-oxa-1,3-diazole)]-6-aminocaproyl-D-erythro-sphingosine | synthetic fluorescent ceramide substrate analogue for fluorescence enzyme assay | Rattus norvegicus | UDP + D-glucosyl-N-[7-(4-nitrobenzo-2-oxa-1,3-diazole)]-6-aminocaproyl-D-erythro-sphingosine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GCS | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Rattus norvegicus |