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Literature summary for 2.4.2.1 extracted from
- Purification and properties of purine nucleoside phosphorylase from Brevibacterium acetylicum ATCC 954 (1991), Agric. Biol. Chem., 55, 493-499.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AgNO3 | - |
Exiguobacterium acetylicum |  |
| CuSO4 | slight | Exiguobacterium acetylicum | |
| HgCl2 | - |
Exiguobacterium acetylicum | |
| NEM | - |
Exiguobacterium acetylicum | |
| PCMB | - |
Exiguobacterium acetylicum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
1 * 31000, SDS-PAGE in absence and presence of 2-mercaptoethanol | Exiguobacterium acetylicum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Exiguobacterium acetylicum | - |
ATCC 954 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Exiguobacterium acetylicum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 195.1 | - |
- |
Exiguobacterium acetylicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2',3'-dideoxyinosine + phosphate | 22% of the activity with inosine | Exiguobacterium acetylicum | alpha-D-2,3-dideoxyribose 1-phosphate + hypoxanthine | - |
? | |
| deoxyguanosine + phosphate | 11% of the activity with inosine | Exiguobacterium acetylicum | guanine + alpha-D-deoxyribose 1-phosphate | - |
? | |
| deoxyinosine + phosphate | 2'-deoxyinosine, 87% of the activity with inosine | Exiguobacterium acetylicum | hypoxanthine + alpha-D-deoxyribose 1-phosphate | - |
r | |
| guanosine + phosphate | 21% of the activity with inosine | Exiguobacterium acetylicum | guanine + alpha-D-ribose 1-phosphate | - |
? | |
| inosine + phosphate | reaction reaches an equilibrium when 21% of the inosine is phosphorolyzed | Exiguobacterium acetylicum | hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
| ribavirin + phosphate | 22% of the activity with inosine. Phosphorolysis reaches an equilibrium when 15% of ribavirin is phosphorolyzed | Exiguobacterium acetylicum | alpha-D-ribose 1-phosphate + 1,2,4-triazole-3-carboxamide | - |
r | |
| xanthosine + phosphate | 26% of the activity with inosine | Exiguobacterium acetylicum | alpha-D-ribose 1-phosphate + xanthine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 31000, SDS-PAGE in absence and presence of 2-mercaptoethanol | Exiguobacterium acetylicum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
phosphorylation of inosine | Exiguobacterium acetylicum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 80 | 30°C: about 35% of maximal activity, 80°C: about 85% of maximal activity | Exiguobacterium acetylicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
phosphorylation of inosine | Exiguobacterium acetylicum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9.5 | pH 6.0: about 60% of maximal activity, pH 9.5: about 80% of maximal activity | Exiguobacterium acetylicum |
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