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Literature summary for 2.4.2.1 extracted from

  • Shi, W.; Ting, L.M.; Kicska, G.A.; Lewandowicz, A.; Tyler, P.C.; Evans, G.B.; Furneaux, R.H.; Kim, K.; Almo, S.C.; Schramm, V.L.
    Plasmodium falciparum purine nucleoside phosphorylase: crystal structures, immucillin inhibitors, and dual catalytic function (2004), J. Biol. Chem., 279, 18103-18106.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme in complex with the inhibitor 5'-methylthio-immucillin-H, hanging-drop vapor-diffusion method Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
5'-methylthio-immucillin-H favors inhibition of Plasmodium falciparum purine nucleoside phosphorylase over human enzyme Homo sapiens
5'-methylthio-immucillin-H favours inhibition of Plasmodium falciparum purine nucleoside phosphorylase over human enzyme Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0047
-
Inosine pH 7.5, 25°C Plasmodium falciparum
0.012
-
Methylthioinosine pH 7.5, 25°C Homo sapiens
0.016
-
Methylthioinosine pH 7.5, 25°C Plasmodium falciparum
0.038
-
Inosine pH 7.5, 25°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P00491
-
-
Plasmodium falciparum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-methylthioinosine + phosphate
-
Homo sapiens hypoxanthine + 5-methylthio-alpha-D-ribose 1-phosphate
-
?
inosine + phosphate
-
Plasmodium falciparum hypoxanthine + alpha-D-ribose 1-phosphate
-
?
inosine + phosphate
-
Homo sapiens hypoxanthine + alpha-D-ribose 1-phosphate
-
?
methylthioinosine + phosphate
-
Plasmodium falciparum methylthiohypoxanthine + alpha-D-ribose 1-phosphate
-
?
methylthioinosine + phosphate
-
Homo sapiens methylthiohypoxanthine + alpha-D-ribose 1-phosphate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
Methylthioinosine pH 7.5, 25°C Homo sapiens
1.5
-
Methylthioinosine pH 7.5, 25°C Plasmodium falciparum
1.7
-
Inosine pH 7.5, 25°C Plasmodium falciparum
31
-
Inosine pH 7.5, 25°C Homo sapiens