Literature summary for 2.4.2.1 extracted from

Ting, L.M.; Gissot, M.; Coppi, A.; Sinnis, P.; Kim, K.
Attenuated Plasmodium yoelii lacking purine nucleoside phosphorylase confer protective immunity (2008), Nat. Med., 14, 954-958.

Search for more literature for 2.4.2.1

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0047	-	Inosine	pH 7.4	Plasmodium falciparum
0.005	-	Methylthioinosine	pH 7.4	Plasmodium yoelii
0.0086	-	Inosine	pH 7.4	Plasmodium yoelii
0.0089	-	guanosine	pH 7.4	Plasmodium yoelii
0.0094	-	guanosine	pH 7.4	Plasmodium falciparum
0.0108	-	Methylthioinosine	pH 7.4	Plasmodium falciparum
0.079	-	2'-deoxyinosine	pH 7.4	Plasmodium yoelii
0.091	-	2'-deoxyinosine	pH 7.4	Plasmodium falciparum

Organism

Organism	UniProt	Comment	Textmining
Plasmodium falciparum	-	-	-
Plasmodium yoelii	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2'-deoxyinosine + phosphate	-	Plasmodium falciparum	alpha-D-2-deoxyribose 1-phosphate + hypoxanthine	-	?
2'-deoxyinosine + phosphate	-	Plasmodium yoelii	alpha-D-2-deoxyribose 1-phosphate + hypoxanthine	-	?
guanosine + phosphate	-	Plasmodium falciparum	guanine + alpha-D-ribose 1-phosphate	-	?
guanosine + phosphate	-	Plasmodium yoelii	guanine + alpha-D-ribose 1-phosphate	-	?
inosine + phosphate	-	Plasmodium falciparum	hypoxanthine + alpha-D-ribose 1-phosphate	-	?
inosine + phosphate	-	Plasmodium yoelii	hypoxanthine + alpha-D-ribose 1-phosphate	-	?
methylthioinosine + phosphate	-	Plasmodium falciparum	methylthiohypoxanthine + alpha-D-ribose 1-phosphate	-	?
methylthioinosine + phosphate	-	Plasmodium yoelii	methylthiohypoxanthine + alpha-D-ribose 1-phosphate	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.9	-	2'-deoxyinosine	pH 7.4	Plasmodium falciparum
1.1	-	Inosine	pH 7.4	Plasmodium falciparum
1.2	-	2'-deoxyinosine	pH 7.4	Plasmodium yoelii
2.6	-	guanosine	pH 7.4	Plasmodium falciparum
2.6	-	Methylthioinosine	pH 7.4	Plasmodium falciparum
3.4	-	Methylthioinosine	pH 7.4	Plasmodium yoelii
3.5	-	Inosine	pH 7.4	Plasmodium yoelii
3.8	-	guanosine	pH 7.4	Plasmodium yoelii

General Information

General Information	Comment	Organism
physiological function	parasites lacking purine nucelotide phosphorylase due to gene disruption are attenuated and clear in mice. Although able to form gametocytes, purine nucelotide phosphorylase-deficient parasites do not form oocysts in mosquito midguts and are not transmitted from mosquitoes to mice. Mice given purine nucelotide phosphorylase-deficient parasites are immune to subsequent challenge to a lethal inoculum of Plasmodium yoelii YM and to challenge from strain Plasmodium yoelii 17XNL	Plasmodium yoelii

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
9.9	-	2'-deoxyinosine	pH 7.4	Plasmodium falciparum
15.2	-	2'-deoxyinosine	pH 7.4	Plasmodium yoelii
234	-	Inosine	pH 7.4	Plasmodium falciparum
240.7	-	Methylthioinosine	pH 7.4	Plasmodium falciparum
289	-	guanosine	pH 7.4	Plasmodium falciparum
4069	-	Inosine	pH 7.4	Plasmodium yoelii
4269	-	guanosine	pH 7.4	Plasmodium yoelii
6800	-	Methylthioinosine	pH 7.4	Plasmodium yoelii

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Release 2023.1 (January 2023)