Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudoalteromonas sp. |
Crystallization (Comment) | Organism |
---|---|
construction of three-dimensional model | Pseudoalteromonas sp. |
Protein Variants | Comment | Organism |
---|---|---|
T156A | about 5fold decrease in kcat/Km | Pseudoalteromonas sp. |
T156S | increase in kcat/Km | Pseudoalteromonas sp. |
T90A | dramatic decrase in kcat/Km | Pseudoalteromonas sp. |
T90A/T156A | 500fold reduction in catalytic activity when compared with wild-type | Pseudoalteromonas sp. |
T90R | increase in kcat/Km | Pseudoalteromonas sp. |
T90R/T156S | 1000fold enhancements in kcat/Km for inosine phosphorolysis | Pseudoalteromonas sp. |
T90S | about 5fold decrease in kcat/Km | Pseudoalteromonas sp. |
V206I | kcat/Km similar to wild-type | Pseudoalteromonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00001 | - |
Inosine | mutant T90R/T156A, pH 7.6, 37°C | Pseudoalteromonas sp. | |
0.00002 | - |
Inosine | mutant T90R, pH 7.6, 37°C | Pseudoalteromonas sp. | |
0.00003 | - |
Inosine | mutant T156S, pH 7.6, 37°C | Pseudoalteromonas sp. | |
0.00004 | - |
Inosine | mutant V206I, pH 7.6, 37°C | Pseudoalteromonas sp. | |
0.00039 | - |
Inosine | wild-type, pH 7.6, 37°C | Pseudoalteromonas sp. | |
0.00092 | - |
Inosine | mutant T90S, pH 7.6, 37°C | Pseudoalteromonas sp. | |
0.00135 | - |
Inosine | mutant T156A, pH 7.6, 37°C | Pseudoalteromonas sp. | |
0.104 | - |
Inosine | mutant T90A/T156A, pH 7.6, 37°C | Pseudoalteromonas sp. | |
143.1 | - |
Inosine | mutant T90A, pH 7.6, 37°C | Pseudoalteromonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudoalteromonas sp. | C8CPR9 | - |
- |
Pseudoalteromonas sp. XM2107 | C8CPR9 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
inosine + phosphate | - |
Pseudoalteromonas sp. | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
inosine + phosphate | - |
Pseudoalteromonas sp. XM2107 | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
additional information | the complete hydrophobic pocket plays an important role in the catalytic function. A potential transition state structure is present in hydrogen bond between the carboxyl groups of Thr90 in the phosphate binding site | Pseudoalteromonas sp. | ? | - |
? | |
additional information | the complete hydrophobic pocket plays an important role in the catalytic function. A potential transition state structure is present in hydrogen bond between the carboxyl groups of Thr90 in the phosphate binding site | Pseudoalteromonas sp. XM2107 | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
Inosine | mutant T90A/T156A, pH 7.6, 37°C | Pseudoalteromonas sp. | |
1.2 | - |
Inosine | mutant T90A, pH 7.6, 37°C | Pseudoalteromonas sp. | |
56.4 | - |
Inosine | mutant T156A, pH 7.6, 37°C | Pseudoalteromonas sp. | |
62.3 | - |
Inosine | mutant T90S, pH 7.6, 37°C | Pseudoalteromonas sp. | |
82.4 | - |
Inosine | mutant V206I, pH 7.6, 37°C | Pseudoalteromonas sp. | |
84.1 | - |
Inosine | wild-type, pH 7.6, 37°C | Pseudoalteromonas sp. | |
98.4 | - |
Inosine | mutant T156S, pH 7.6, 37°C | Pseudoalteromonas sp. | |
164.4 | - |
Inosine | mutant T90R, pH 7.6, 37°C | Pseudoalteromonas sp. | |
324.3 | - |
Inosine | mutant T90R/T156A, pH 7.6, 37°C | Pseudoalteromonas sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.082 | - |
Inosine | mutant T90A/T156A, pH 7.6, 37°C | Pseudoalteromonas sp. | |
0.087 | - |
Inosine | mutant T90A, pH 7.6, 37°C | Pseudoalteromonas sp. | |
41.8 | - |
Inosine | mutant T156A, pH 7.6, 37°C | Pseudoalteromonas sp. | |
67.8 | - |
Inosine | mutant T90S, pH 7.6, 37°C | Pseudoalteromonas sp. | |
189 | - |
Inosine | mutant V206I, pH 7.6, 37°C | Pseudoalteromonas sp. | |
215 | - |
Inosine | wild-type, pH 7.6, 37°C | Pseudoalteromonas sp. | |
3540 | - |
Inosine | mutant T156S, pH 7.6, 37°C | Pseudoalteromonas sp. | |
11500 | - |
Inosine | mutant T90R, pH 7.6, 37°C | Pseudoalteromonas sp. | |
43500 | - |
Inosine | mutant T90R/T156A, pH 7.6, 37°C | Pseudoalteromonas sp. |