Literature summary for 2.4.2.10 extracted from

  • Krungkrai, S.R.; Aoki, S.; Palacpac, N.M.; Sato, D.; Mitamura, T.; Krungkrai, J.; Horii, T.
    Human malaria parasite orotate phosphoribosyltransferase: functional expression, characterization of kinetic reaction mechanism and inhibition profile (2004), Mol. Biochem. Parasitol., 134, 245-255.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Plasmodium falciparum

General Stability

General Stability Organism
recombinant enzyme, stable for at least 3 months at 4°C or -20°C in presence of 1 mM dithiothreitol and 10% glycerol Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
5-aminoorotate 50% inhibition at 0.01 mM Plasmodium falciparum
5-bromoorotate
-
Plasmodium falciparum
5-Fluoroorotate 50% inhibition at 2-10 nM Plasmodium falciparum
5-fluorouracil 50% inhibition at 0.005-0.01 mM Plasmodium falciparum
5-iodoorotate
-
Plasmodium falciparum
5-Methylorotate 50% inhibition at 0.001 mM Plasmodium falciparum
5-phospho-alpha-D-ribose 1-diphosphate product inhibition, competitive to diphosphate, competitive to orotidine 5'-phosphate Plasmodium falciparum
diphosphate product inhibition, noncompetitive to orotate, competitive to 5-phospho-alpha-D-ribose 1-diphosphate Plasmodium falciparum
Orotate product inhibition, noncompetitive to diphosphate, competitive to orotidine 5'-phosphate Plasmodium falciparum
orotidine 5'-phosphate product inhibition, competitive to 5-phospho-alpha-D-ribose 1-diphosphate, competitive to orotate Plasmodium falciparum
pyrazofurin 50% inhibition at 0.006-0.024 mM Plasmodium falciparum
Uracil
-
Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0143
-
diphosphate pH 8.0, 37°C Plasmodium falciparum
0.025
-
5-Fluoroorotate pH 8.0, 37°C Plasmodium falciparum
0.0182
-
Orotate pH 8.0, 37°C Plasmodium falciparum
0.0251
-
orotidine 5'-phosphate pH 8.0, 37°C Plasmodium falciparum
0.0282
-
5-phospho-alpha-D-ribose 1-diphosphate pH 8.0, 37°C Plasmodium falciparum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
gel filtration, recombinant enzyme Plasmodium falciparum
33000
-
2 * 33400, recombinant enzyme, SDS-PAGE, 2 * 33000, calculated Plasmodium falciparum
33400
-
2 * 33400, recombinant enzyme, SDS-PAGE, 2 * 33000, calculated Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum Q8N0R1
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
orotate + 5-phospho-alpha-D-ribose 1-diphosphate
-
Plasmodium falciparum orotidine 5'-phosphate + diphosphate
-
r
5-fluoroorotate + 5-phospho-alpha-D-ribose 1-diphosphate
-
Plasmodium falciparum ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 33400, recombinant enzyme, SDS-PAGE, 2 * 33000, calculated Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6
-
orotidine 5'-phosphate pH 8.0, 37°C Plasmodium falciparum
1.3
-
diphosphate pH 8.0, 37°C Plasmodium falciparum
3
-
Orotate pH 8.0, 37°C Plasmodium falciparum
3.2
-
5-phospho-alpha-D-ribose 1-diphosphate pH 8.0, 37°C Plasmodium falciparum
4
-
5-Fluoroorotate pH 8.0, 37°C Plasmodium falciparum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0075
-
Orotate pH 8.0, 37°C, cosubstrate orotidine 5'-phosphate Plasmodium falciparum
0.125
-
diphosphate pH 8.0, 37°C, cosubstrate 5-phospho-alpha-D-ribose 1-diphosphate Plasmodium falciparum
0.0482
-
5-bromoorotate pH 8.0, 37°C Plasmodium falciparum
0.0153
-
5-iodoorotate pH 8.0, 37°C Plasmodium falciparum
0.0157
-
orotidine 5'-phosphate pH 8.0, 37°C, cosubstrate 5-phospho-alpha-D-ribose 1-diphosphate Plasmodium falciparum
0.0213
-
Orotate pH 8.0, 37°C, cosubstrate diphosphate Plasmodium falciparum
0.0423
-
5-phospho-alpha-D-ribose 1-diphosphate pH 8.0, 37°C, cosubstrate diphosphate Plasmodium falciparum
0.0241
-
orotidine 5'-phosphate pH 8.0, 37°C, cosubstrate orotate Plasmodium falciparum
0.0797
-
diphosphate pH 8.0, 37°C, cosubstrate orotate Plasmodium falciparum
0.1501
-
5-phospho-alpha-D-ribose 1-diphosphate pH 8.0, 37°C, cosubstrate orotidine 5'-phosphate Plasmodium falciparum
0.0954
-
5-Fluoroorotate pH 8.0, 37°C Plasmodium falciparum
0.0214
-
5-Methylorotate pH 8.0, 37°C Plasmodium falciparum
0.1212
-
Uracil pH 8.0, 37°C Plasmodium falciparum
0.0561
-
5-fluorouracil pH 8.0, 37°C Plasmodium falciparum
0.0705
-
pyrazofurin pH 8.0, 37°C Plasmodium falciparum

pI Value

Organism Comment pI Value Maximum pI Value
Plasmodium falciparum calculated
-
7.1