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Literature summary for 2.4.2.14 extracted from
- Properties of 5-phosphoribosyl-1-pyrophosphate amidotransferase from human lymphoblasts (1973), J. Biol. Chem., 248, 138-143.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Mg-phosphoribosyldiphosphate | - |
Homo sapiens |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AMP | 1.8 mM, 50% inhibition | Homo sapiens | |
| GMP | 0.5 mM; 50% inhibition | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.6 | - |
L-glutamine | - |
Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Homo sapiens | |
| Mg2+ | maximal activation at concentrations 2.5-5 times higher than that of the corresponding 5-phospho-alpha-D-ribose 1-diphosphate concentration | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| lymphoblast | diploid wild 2 line, tissue culture | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00125 | 0.0014 | - |
Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C or 4°C, cell-free extract, overnight, more than 50% loss of activity | Homo sapiens |
| -20°C, crude, more than 50% loss of activity overnight | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | - |
Homo sapiens | 5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
at least 15 min stable | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 7.4 | Tris/HCl buffer preferred | Homo sapiens |
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