General Stability | Organism |
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overview, stability of the enzyme at various pH-values, salt concentrations and histidine concentrations | Salmonella enterica subsp. enterica serovar Typhimurium |
slight stabilization by 10 mM MgCl2 or CaCl2 by 1 mM MnCl2 and by 1 mM histidine at pH-values above 7 | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-histidine | feed-back inhibition | Salmonella enterica subsp. enterica serovar Typhimurium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
6 * 33000, SDS-disc gel electrophoresis | Salmonella enterica subsp. enterica serovar Typhimurium |
216000 | - |
ultracentrifugation analysis | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5-phospho-alpha-D-ribose 1-diphosphate | Salmonella enterica subsp. enterica serovar Typhimurium | first step in histidine biosynthesis | diphosphate + N-1-(5'-phosphoribosyl)-ATP | - |
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Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
first purification by heating the crude extract, ammonium sulfate precipitation and selective histidine-dependent ammonium sulfate precipitation | Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
description of assay method | Salmonella enterica subsp. enterica serovar Typhimurium |
Storage Stability | Organism |
---|---|
4°C, HEPES buffer pH 7.5, final ammonium sulfate microcrystalline enzyme in 60% saturated ammonium sulfate, 0.1 M NaCl, 0.01 M Tris, 1.5 mM EDTA, 10 mM dithiothreitol, stable for 1 month, greater than 95% activity retained | Salmonella enterica subsp. enterica serovar Typhimurium |
storage in liquid nitrogen of quick-frozen enzyme in HEPES buffer pH 7.5, 0.1 M NaCl, 0.01 M Tris, 0.5 mM EDTA, 1 mM dithiothreitol, 1 mM histidine, indefinitely stable, preserves 100% activity. The critical factor for stability seems to be the maintenance of a sulfhydryl-reducing environment, high dithiothreitol concentrations has no adverse effect at either 0°C or 37°C | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 1-(5-phospho-D-ribosyl)-ATP + diphosphate | - |
? | |
ATP + 5-phospho-alpha-D-ribose 1-diphosphate | first step in histidine biosynthesis | Salmonella enterica subsp. enterica serovar Typhimurium | diphosphate + N-1-(5'-phosphoribosyl)-ATP | - |
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Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 33000, SDS-disc gel electrophoresis | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
overview: stability at various pH-values | Salmonella enterica subsp. enterica serovar Typhimurium |