Cloned (Comment) | Organism |
---|---|
codon adapted to Escherichia coli, synthetically prepared, His-tagged protein expressed in Escherichia coli BL21(DE3)pLysS | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Mycobacterium tuberculosis | |
L-histidine | allosteric inhibitor, inhibition dependent on pH, uncompetitive versus ATP, noncompetitive versus 5-phospho-alpha-D-ribosyl diphosphate | Mycobacterium tuberculosis | |
Mg2+ | at high concentrations, Ki = 23 mM | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.049 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 8.5, 25°C | Mycobacterium tuberculosis | |
0.263 | - |
ATP | pH 8.5, 25°C | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | - |
Mycobacterium tuberculosis | |
Mg2+ | Kact = 1.9 mM; Ki = 23 mM | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
180000 | - |
gel filtration | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography (Ni2+) | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Mycobacterium tuberculosis | 1-(5-phospho-alpha-D-ribosyl)-ATP + diphosphate | - |
r | |
ATP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Mycobacterium tuberculosis H37Rv | 1-(5-phospho-alpha-D-ribosyl)-ATP + diphosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
homohexamer | 6 * 31515.1, ESI-MS, gel filtration, His-tagged protein, not influenced by allosteric inhibitor L-histidine or ATP | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
ATP phosphoribosyltransferase | - |
Mycobacterium tuberculosis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
L-histidine | uncompetitive versus ATP, pH 8.0, 25°C | Mycobacterium tuberculosis | |
0.0079 | - |
L-histidine | uncompetitive versus ATP, pH 8.25, 25°C | Mycobacterium tuberculosis | |
0.0235 | - |
L-histidine | Kii-value, non competitive versus 5-phospho-alpha-D-ribosyl diphosphate, pH 8.5, 25°C | Mycobacterium tuberculosis | |
0.0257 | - |
L-histidine | Kis-value, non competitive versus 5-phospho-alpha-D-ribosyl diphosphate, pH 8.5, 25°C | Mycobacterium tuberculosis | |
0.0269 | - |
L-histidine | uncompetitive versus ATP, pH 8.75, 25°C | Mycobacterium tuberculosis | |
0.0279 | - |
L-histidine | uncompetitive versus ATP, pH 8.5, 25°C | Mycobacterium tuberculosis | |
0.0504 | - |
L-histidine | uncompetitive versus ATP, pH 9.0, 25°C | Mycobacterium tuberculosis | |
0.1094 | - |
L-histidine | uncompetitive versus ATP, pH 9.25, 25°C | Mycobacterium tuberculosis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.033 | - |
pH 8.5, 25°C | Mycobacterium tuberculosis | L-histidine |