Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Campylobacter jejuni |
Crystallization (Comment) | Organism |
---|---|
truncated long-form enzyme devoid of its regulatory domain as apoenzyme and in complex with ATP, 5-phospho-alpha-D-ribose 1-diphosphate or 1-(5-phospho-beta-D-ribosyl)-ATP, hanging drop vapor diffusion method, using 1 M sodium acetate, 10 mM ZnCl2, and 7-10% (w/v) PEG 6000 (pH 5.0) | Campylobacter jejuni |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-(5-phospho-beta-D-ribosyl)-ATP | product inhibition | Campylobacter jejuni | |
AMP | competitive inhibition | Campylobacter jejuni | |
L-histidine | allosterical inhibition | Campylobacter jejuni |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
5-phospho-alpha-D-ribose 1-diphosphate | wild type long-form enzyme, at pH 8.5 and 25°C | Campylobacter jejuni | |
0.028 | - |
5-phospho-alpha-D-ribose 1-diphosphate | truncated long-form enzyme devoid of its regulatory domain, at pH 8.5 and 25°C | Campylobacter jejuni | |
0.097 | - |
ATP | wild type long-form enzyme, at pH 8.5 and 25°C | Campylobacter jejuni | |
0.117 | - |
ATP | truncated long-form enzyme devoid of its regulatory domain, at pH 8.5 and 25°C | Campylobacter jejuni |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 10 mM used in assay conditions | Campylobacter jejuni |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25308 | - |
truncated long-form enzyme devoid of its regulatory domain, calculated from amino acid sequence | Campylobacter jejuni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate | Campylobacter jejuni | - |
ATP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate | Campylobacter jejuni RM 1221 | - |
ATP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
ATP + 5-phospho-alpha-D-ribose 1-diphosphate | Campylobacter jejuni | - |
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate | - |
r | |
ATP + 5-phospho-alpha-D-ribose 1-diphosphate | Campylobacter jejuni RM 1221 | - |
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Campylobacter jejuni | Q5HSJ4 | - |
- |
Campylobacter jejuni RM 1221 | Q5HSJ4 | - |
- |
Purification (Comment) | Organism |
---|---|
HiTrap Talon resin column chromatography, GST-affinity column chromatography and Superdex S200 gel filtration | Campylobacter jejuni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate | - |
Campylobacter jejuni | ATP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate | - |
Campylobacter jejuni RM 1221 | ATP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
ATP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Campylobacter jejuni | 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate | - |
r | |
ATP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Campylobacter jejuni RM 1221 | 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
homohexamer | long enzyme form | Campylobacter jejuni |
Synonyms | Comment | Organism |
---|---|---|
adenosine triphosphate phosphoribosyltransferase | - |
Campylobacter jejuni |
ATP-phosphoribosyltransferase | - |
Campylobacter jejuni |
ATP-PRT | - |
Campylobacter jejuni |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
52.5 | - |
melting temperature of the truncated long-form enzyme devoid of its regulatory domain | Campylobacter jejuni |
60.7 | - |
melting temperature of the wild type long-form enzyme | Campylobacter jejuni |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
ATP | truncated long-form enzyme devoid of its regulatory domain, at pH 8.5 and 25°C | Campylobacter jejuni | |
0.024 | - |
5-phospho-alpha-D-ribose 1-diphosphate | truncated long-form enzyme devoid of its regulatory domain, at pH 8.5 and 25°C | Campylobacter jejuni | |
1.8 | - |
ATP | wild type long-form enzyme, at pH 8.5 and 25°C | Campylobacter jejuni | |
1.8 | - |
5-phospho-alpha-D-ribose 1-diphosphate | wild type long-form enzyme, at pH 8.5 and 25°C | Campylobacter jejuni |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0068 | - |
truncated long-form enzyme devoid of its regulatory domain, at pH 8.5 and 25°C | Campylobacter jejuni | 1-(5-phospho-beta-D-ribosyl)-ATP | |
0.04 | - |
wild type long-form enzyme, at pH 8.5 and 25°C | Campylobacter jejuni | 1-(5-phospho-beta-D-ribosyl)-ATP | |
0.044 | - |
wild type long-form enzyme, at pH 8.5 and 25°C | Campylobacter jejuni | L-histidine | |
0.42 | - |
truncated long-form enzyme devoid of its regulatory domain, at pH 8.5 and 25°C | Campylobacter jejuni | AMP | |
2.1 | - |
wild type long-form enzyme, at pH 8.5 and 25°C | Campylobacter jejuni | AMP |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | - |
ATP | truncated long-form enzyme devoid of its regulatory domain, at pH 8.5 and 25°C | Campylobacter jejuni | |
0.86 | - |
5-phospho-alpha-D-ribose 1-diphosphate | truncated long-form enzyme devoid of its regulatory domain, at pH 8.5 and 25°C | Campylobacter jejuni | |
19 | - |
ATP | wild type long-form enzyme, at pH 8.5 and 25°C | Campylobacter jejuni | |
120 | - |
5-phospho-alpha-D-ribose 1-diphosphate | wild type long-form enzyme , at pH 8.5 and 25°C | Campylobacter jejuni |