Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) pLysS cells | Mycoplasma hyorhinis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.095 | - |
uridine | at pH 7.6 and 37°C | Mycoplasma hyorhinis | |
0.365 | - |
thymidine | at pH 7.6 and 37°C | Mycoplasma hyorhinis | |
0.856 | - |
5-trifluorothymidine | at pH 7.6 and 37°C | Mycoplasma hyorhinis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mycoplasma hyorhinis | the enzyme also displays (2'-deoxy)ribosyltransferase activity | ? | - |
? | |
additional information | Mycoplasma hyorhinis ATCC 17981 | the enzyme also displays (2'-deoxy)ribosyltransferase activity | ? | - |
? | |
thymidine + phosphate | Mycoplasma hyorhinis | - |
thymine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? | |
thymidine + phosphate | Mycoplasma hyorhinis ATCC 17981 | - |
thymine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? | |
uridine + phosphate | Mycoplasma hyorhinis | - |
uracil + alpha-D-ribose 1-phosphate | - |
? | |
uridine + phosphate | Mycoplasma hyorhinis ATCC 17981 | - |
uracil + alpha-D-ribose 1-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycoplasma hyorhinis | - |
- |
- |
Mycoplasma hyorhinis ATCC 17981 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-trifluorothymidine + phosphate | - |
Mycoplasma hyorhinis | 5-trifluorothymine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? | |
5-trifluorothymidine + phosphate | - |
Mycoplasma hyorhinis ATCC 17981 | 5-trifluorothymine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? | |
additional information | the enzyme also displays (2'-deoxy)ribosyltransferase activity | Mycoplasma hyorhinis | ? | - |
? | |
additional information | the enzyme also displays (2'-deoxy)ribosyltransferase activity | Mycoplasma hyorhinis ATCC 17981 | ? | - |
? | |
thymidine + phosphate | - |
Mycoplasma hyorhinis | thymine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? | |
thymidine + phosphate | - |
Mycoplasma hyorhinis ATCC 17981 | thymine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? | |
uridine + phosphate | - |
Mycoplasma hyorhinis | uracil + alpha-D-ribose 1-phosphate | - |
? | |
uridine + phosphate | - |
Mycoplasma hyorhinis ATCC 17981 | uracil + alpha-D-ribose 1-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PYNP | - |
Mycoplasma hyorhinis |
pyrimidine nucleoside phosphorylase | - |
Mycoplasma hyorhinis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.25 | - |
5-trifluorothymidine | at pH 7.6 and 37°C | Mycoplasma hyorhinis | |
9.03 | - |
uridine | at pH 7.6 and 37°C | Mycoplasma hyorhinis | |
20.5 | - |
thymidine | at pH 7.6 and 37°C | Mycoplasma hyorhinis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10 | - |
5-trifluorothymidine | at pH 7.6 and 37°C | Mycoplasma hyorhinis | |
56.7 | - |
thymidine | at pH 7.6 and 37°C | Mycoplasma hyorhinis | |
95 | - |
uridine | at pH 7.6 and 37°C | Mycoplasma hyorhinis |