BRENDA - Enzyme Database
show all sequences of 2.4.2.28

Selective killing of tumors deficient in methylthioadenosine phosphorylase: a novel strategy

Lubin, M.; Lubin, A.; PLoS ONE 4, e5735 (2009)

Data extracted from this reference:

Application
Application
Commentary
Organism
drug development
combination of 5'-methylthioadenosine with clinical cancer drugs 5-fluorouracil or 6-thioguanine, in the treatment of MTAP-negative tumors, may produce a significantly improved therapeutic index
Homo sapiens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
ML-1 cell
-
Homo sapiens
-
additional information
A549 cells and MCF-7 cells are MTAP-negative cells
Homo sapiens
-
skin fibroblast
HF cell
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5'-deoxyadenosine + phosphate
-
706428
Homo sapiens
adenine + 5-deoxy-D-ribose 1-phosphate
-
-
-
?
5'-methylthioadenosine + phosphate
-
706428
Homo sapiens
adenine + 5-methylthio-D-ribose 1-phosphate
-
-
-
?
Application (protein specific)
Application
Commentary
Organism
drug development
combination of 5'-methylthioadenosine with clinical cancer drugs 5-fluorouracil or 6-thioguanine, in the treatment of MTAP-negative tumors, may produce a significantly improved therapeutic index
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
ML-1 cell
-
Homo sapiens
-
additional information
A549 cells and MCF-7 cells are MTAP-negative cells
Homo sapiens
-
skin fibroblast
HF cell
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5'-deoxyadenosine + phosphate
-
706428
Homo sapiens
adenine + 5-deoxy-D-ribose 1-phosphate
-
-
-
?
5'-methylthioadenosine + phosphate
-
706428
Homo sapiens
adenine + 5-methylthio-D-ribose 1-phosphate
-
-
-
?
General Information
General Information
Commentary
Organism
physiological function
MTAP substrates protect MTAP-positive cells from toxicity of adenine analogs 2,6-diaminopurine, 6-methylpurine, and 2-fluoroadenine and clinical cancer drugs 5-fluorouracil or 6-thioguanine. Adenine analog plus 5'-methylthioadenosine, kills MTAP-negative A549 lung tumor cells, while MTAP-positive human fibroblasts are protected, whereas in co-cultures of the breast tumor cell line, MCF-7, and HF cells, MCF-7 is inhibited or killed, while HF cells proliferate robustly
Homo sapiens
General Information (protein specific)
General Information
Commentary
Organism
physiological function
MTAP substrates protect MTAP-positive cells from toxicity of adenine analogs 2,6-diaminopurine, 6-methylpurine, and 2-fluoroadenine and clinical cancer drugs 5-fluorouracil or 6-thioguanine. Adenine analog plus 5'-methylthioadenosine, kills MTAP-negative A549 lung tumor cells, while MTAP-positive human fibroblasts are protected, whereas in co-cultures of the breast tumor cell line, MCF-7, and HF cells, MCF-7 is inhibited or killed, while HF cells proliferate robustly
Homo sapiens
Other publictions for EC 2.4.2.28
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736501
Vodnala
Trypanosoma brucei methylthioa ...
Trypanosoma brucei, Trypanosoma brucei TC221
J. Biol. Chem.
291
11717-11726
2016
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736923
Bistulfi
The essential role of methylth ...
Homo sapiens
Oncotarget
7
14380-14393
2016
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731366
Bagarolo
Multiple disulfide bridges mod ...
Saccharolobus solfataricus, Saccharolobus solfataricus P2
Biochim. Biophys. Acta
1854
1458-1465
2015
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736943
Becker
Expression of methylthioadenos ...
Homo sapiens
Pathobiology
82
84-89
2015
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726889
Kang
Crystal structure and biochemi ...
Brucella melitensis
Biochem. Biophys. Res. Commun.
446
965-970
2014
-
-
1
1
18
-
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12
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12
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12
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735675
Guan
Thermodynamic analysis of tran ...
Homo sapiens
Biochemistry
52
8313-8322
2013
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1
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4
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736173
Lv
Association between rs10118757 ...
Homo sapiens
Gene
526
344-346
2013
-
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737102
Bartasun
A study on the interaction of ...
uncultured bacterium
PLoS ONE
8
e55697
2013
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1
1
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1
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737123
Czech
Expression and function of met ...
Homo sapiens
PLoS ONE
8
e80703
2013
-
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1
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1
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721167
Zhang
A corrected space group for Su ...
Saccharolobus solfataricus, Saccharolobus solfataricus P2
Acta Crystallogr. Sect. D
68
249-252
2012
-
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1
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721732
Cacciapuoti
Role of disulfide bonds in con ...
Saccharolobus solfataricus
Biochim. Biophys. Acta
1824
1136-1143
2012
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1
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723794
Buckoreelall
Identification of Rv0535 as me ...
Mycobacterium tuberculosis
Tuberculosis
92
139-147
2012
-
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1
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2
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2
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7
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721628
Guan
Entropy-driven binding of pico ...
Homo sapiens
Biochemistry
50
10408-10417
2011
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1
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721717
Cacciapuoti
Unraveling the structural and ...
Pyrococcus furiosus
Biochim. Biophys. Acta
1814
1358-1366
2011
-
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1
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4
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705308
Sommer
Methylthioadenosine phosphoryl ...
Homo sapiens
J. Pathol.
220
608-617
2010
-
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715849
Longshaw
Design and synthesis of potent ...
Escherichia coli
J. Med. Chem.
53
6730-6746
2010
-
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17
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700355
Gutierrez
Transition state analogs of 5' ...
Escherichia coli, Escherichia coli O157:H7 EDL933, Vibrio harveyi
Nat. Chem. Biol.
5
251-257
2009
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2
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1
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702045
Park
Biochemical and structural cha ...
Arabidopsis thaliana
Biochem. Biophys. Res. Commun.
381
619-624
2009
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1
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703023
Kadariya
Mice heterozygous for germ-lin ...
Mus musculus
Cancer Res.
69
5961-5969
2009
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706428
Lubin
Selective killing of tumors de ...
Homo sapiens
PLoS ONE
4
e5735
2009
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1
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681477
Siu
Molecular determinants of subs ...
Arabidopsis thaliana, Escherichia coli
J. Mol. Biol.
378
112-128
2008
1
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6
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2
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2
684796
Alhebshi
Methylthioadenosine phosphoryl ...
Homo sapiens
Asian Pac. J. Cancer Prev.
9
291-294
2008
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1
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684999
Fernandez-Irigoyen
Redox regulation of methylthio ...
Homo sapiens, Mus musculus
Biochem. J.
411
457-465
2008
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2
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688782
Watanabe
Immunohistochemical diagnosis ...
Homo sapiens
Lung Cancer
63
39-44
2008
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
698460
Luo
Ribosyl geometry in the transi ...
Streptococcus pneumoniae
J. Am. Chem. Soc.
130
11617-11619
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
700813
Oh
The Arabidopsis calcium sensor ...
Arabidopsis thaliana
Plant Physiol.
148
1883-1896
2008
-
-
1
-
-
-
1
-
1
-
1
-
-
1
-
-
1
-
-
5
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
1
-
5
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
684288
Williamson
Methylthioadenosine phosphoryl ...
Homo sapiens
Am. J. Med. Genet. A
143A
1630-1639
2007
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687178
Miyazaki
Methylthioadenosine phosphoryl ...
Homo sapiens
Int. J. Oncol.
31
1069-1076
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
7
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687575
Basu
A transition state analogue of ...
Homo sapiens
J. Biol. Chem.
282
21477-21486
2007
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
-
-
-
-
7
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
7
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
671628
Wild
Tissue microarray analysis of ...
Homo sapiens
Arch. Dermatol.
142
471-476
2006
-
2
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
5
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
5
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
674184
Singh
Transition-state structure of ...
Homo sapiens
J. Am. Chem. Soc.
128
14691-14696
2006
-
-
1
-
-
-
-
1
-
-
-
1
-
2
-
-
-
1
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
720229
Zhang
The crystal structure of 5-deo ...
Saccharolobus solfataricus
J. Mol. Biol.
357
252-262
2006
-
-
-
1
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658283
Kung
Design, synthesis, and biologi ...
Homo sapiens
Bioorg. Med. Chem. Lett.
15
2829-2833
2005
-
-
-
-
-
-
-
28
-
-
-
-
-
2
-
-
-
-
-
-
-
-
33
-
-
-
-
29
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
28
-
-
-
-
-
-
-
-
-
-
-
-
33
-
-
-
-
29
-
-
-
-
-
-
-
-
-
-
658681
Cacciapuoti
A novel hyperthermostable 5'-d ...
Saccharolobus solfataricus
FEBS J.
272
1886-1899
2005
-
-
1
-
2
-
-
4
-
-
2
-
-
5
-
-
-
-
-
-
-
-
4
1
1
1
4
4
-
-
-
-
-
1
-
-
-
1
-
-
2
-
-
-
-
4
-
-
2
-
-
-
-
-
-
-
-
-
4
1
1
1
4
4
-
-
-
1
-
-
-
-
-
-
675281
Evans
Second generation transition s ...
Homo sapiens
J. Med. Chem.
48
4679-4689
2005
-
-
-
-
-
-
17
1
-
-
-
1
-
2
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
17
-
-
-
-
-
-
-
-
-
-
17
17
1
-
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
675912
Karikari
Homozygous deletions of methyl ...
Homo sapiens
Mol. Cancer Ther.
4
1860-1866
2005
-
-
1
-
1
-
-
-
1
-
-
1
-
2
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676236
Parker
Design and evaluation of 5-mod ...
Homo sapiens
Nucleosides Nucleotides Nucleic Acids
24
387-392
2005
-
-
-
-
-
-
1
-
-
-
-
1
-
3
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658069
Lee
Structural comparison of MTA p ...
Homo sapiens
Biochemistry
43
5159-5169
2004
-
-
-
-
-
-
1
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658091
Singh
Picomolar transition state ana ...
Homo sapiens
Biochemistry
43
9-18
2004
-
-
-
1
-
-
3
1
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
1
-
-
-
3
4
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658650
Cacciapuoti
Methylthioadenosine phosphoryl ...
Pyrococcus furiosus
Eur. J. Biochem.
271
4834-4844
2004
-
-
1
-
-
1
-
-
-
-
-
-
-
4
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659678
Evans
Targeting the polyamine pathwa ...
Homo sapiens
J. Med. Chem.
47
3275-3281
2004
-
-
-
-
-
-
16
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
16
-
-
-
-
-
-
-
-
-
-
16
16
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
637556
Cacciapuoti
Purification and characterizat ...
Pyrococcus furiosus
Extremophiles
7
159-168
2003
-
-
-
-
-
2
1
4
-
-
2
-
6
8
-
-
1
-
-
-
1
1
5
1
2
2
6
4
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
4
-
-
2
-
6
-
-
1
-
-
1
1
5
1
2
2
6
4
1
-
-
-
-
-
-
-
-
-
637557
Behrmann
Characterization of methylthio ...
Homo sapiens
Am. J. Pathol.
163
683-690
2003
-
-
1
-
2
-
1
-
-
-
-
1
-
2
-
-
-
-
-
11
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
11
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659289
Subhi
Methylthioadenosine phosphoryl ...
Saccharomyces cerevisiae
J. Biol. Chem.
278
49868-49873
2003
-
-
1
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
637558
Kadariya
Deletion of dinucleotide repea ...
Homo sapiens
Jpn. J. Cancer Res.
93
369-373
2002
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
637554
Cacciapuoti
Conformational changes and sta ...
Saccharolobus solfataricus
Extremophiles
5
295-302
2001
-
-
1
1
-
3
2
-
-
-
1
-
-
3
-
-
1
-
-
-
-
-
2
1
-
-
3
-
1
-
-
-
-
-
-
-
-
1
-
1
-
3
-
2
-
-
-
-
1
-
-
-
-
1
-
-
-
-
2
1
-
-
3
-
1
-
-
-
-
-
-
-
-
-
637555
Appleby
Three-dimensional structure of ...
Saccharolobus solfataricus
J. Biol. Chem.
276
39232-39242
2001
-
-
-
1
-
-
-
-
-
-
-
-
-
4
-
-
1
1
-
-
-
-
2
2
1
-
4
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
2
2
-
4
-
-
-
-
-
-
-
-
-
-
-
637552
Slominska
The role of 5'-deoxy-5'-methyl ...
Homo sapiens, Rattus norvegicus, Rattus norvegicus Wistar, Sus scrofa
Adv. Exp. Med. Biol.
486
159-162
2000
-
-
-
-
-
-
-
2
-
-
-
1
-
168
-
-
-
-
-
3
3
-
9
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
3
3
-
9
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
637553
Tang
Defects in methylthioadenosine ...
Homo sapiens
Cancer Res.
60
5543-5547
2000
-
-
1
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
7
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
7
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
637549
Cacciapuoti
Heterologous expression of 5'- ...
Saccharolobus solfataricus
Protein Expr. Purif.
16
125-135
1999
-
-
1
-
-
-
3
2
-
-
2
-
-
5
-
-
1
-
-
-
2
-
2
1
3
2
6
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
3
-
4
-
-
4
-
-
-
-
2
-
-
2
-
2
2
4
2
9
-
-
-
-
-
-
-
-
-
-
-
637550
Bacchi
Metabolic effects of a methylt ...
Trypanosoma brucei brucei
Biochem. Pharmacol.
57
89-96
1999
-
-
-
-
-
-
-
-
-
-
-
3
-
2
-
-
-
-
-
-
4
-
8
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
4
-
8
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
637551
Appleby
The structure of human 5'-deox ...
Homo sapiens
Structure
7
629-641
1999
-
1
1
1
-
-
-
-
-
-
-
2
-
2
-
-
1
1
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
637548
Yu
Presence of methylthioadenosin ...
Homo sapiens
Clin. Cancer Res.
3
433-438
1997
-
1
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
5
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
5
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
724984
Porcelli
Non-thermal effects of microwa ...
Saccharolobus solfataricus
FEBS Lett.
402
102-106
1997
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
637546
Della Ragione
Purification and characterizat ...
Homo sapiens
Biochem. Biophys. Res. Commun.
223
514-519
1996
-
1
1
-
-
-
2
2
-
-
2
-
-
3
-
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1
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637547
Nobori
Genomic cloning of methylthioa ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
93
6203-6208
1996
-
-
1
-
-
-
-
-
-
-
-
1
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3
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3
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-
-
-
-
679624
Cacciapuoti
Extremely thermophilic and the ...
Saccharolobus solfataricus
Eur. J. Biochem.
239
632-637
1996
-
-
1
-
-
-
-
-
-
-
1
-
-
4
-
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-
-
-
-
637544
Cacciapuoti
Purification and characterizat ...
Saccharolobus solfataricus
J. Biol. Chem.
269
24762-24769
1994
-
-
-
-
-
2
3
2
1
-
2
5
11
5
-
-
1
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2
2
13
2
2
2
5
-
2
1
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1
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2
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3
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4
2
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4
5
13
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2
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2
2
13
3
3
2
7
-
3
1
-
1
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637545
Fabianowska-Majewska
Substrate specificity of methy ...
Homo sapiens
Acta Biochim. Pol.
41
391-395
1994
-
-
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4
3
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1
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1
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3
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8
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3
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8
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-
637529
Lee
-
Purification and properties of ...
Rattus norvegicus
Korean Biochem. J.
26
433-439
1993
4
-
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1
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-
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1
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2
1
1
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1
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1
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637530
Cacciapuoti
-
Thermophilicity and thermostab ...
Saccharolobus solfataricus
Life Chem. Rep.
10
75-81
1992
-
-
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-
-
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-
1
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1
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4
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1
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1
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4
-
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-
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637531
Toorchen
Purification and characterizat ...
Homo sapiens
Biochem. Pharmacol.
41
2023-2030
1991
-
-
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-
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2
-
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2
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1
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2
1
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1
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2
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1
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1
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1
1
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-
-
-
637532
Savarese
5'-Deoxy-5'-methylthioadenosin ...
Homo sapiens, Mus musculus
Biochem. Pharmacol.
40
2465-2471
1990
-
-
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-
-
10
-
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-
2
-
4
-
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1
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2
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7
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10
7
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2
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1
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-
-
-
-
-
-
637543
Della Ragione
Physicochemical and immunologi ...
Bos taurus
J. Biol. Chem.
265
6241-6246
1990
-
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-
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2
-
-
4
-
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2
1
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1
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2
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1
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2
1
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2
1
1
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-
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637533
Ghoda
Substrate specificities of 5'- ...
Trypanosoma brucei brucei
Mol. Biochem. Parasitol.
27
109-118
1988
-
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3
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5
-
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-
-
637534
Koszalka
-
5'-Methyladenosine (MTA) phosp ...
Leishmania donovani
Adv. Exp. Med. Biol.
195B
559-563
1986
-
-
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-
5
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1
1
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1
-
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1
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7
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1
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1
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7
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1
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-
-
-
-
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-
-
637542
Della Ragione
Purification and characterizat ...
Homo sapiens
J. Biol. Chem.
261
12324-12329
1986
2
-
-
-
-
2
6
5
-
-
2
-
-
2
-
-
1
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2
1
1
1
1
1
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3
-
2
1
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2
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2
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6
-
5
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2
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1
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2
1
1
1
1
1
-
3
-
2
1
-
-
-
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-
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-
-
637539
Zappia
-
Purification and properties of ...
Caldariella acidophila
Methods Enzymol.
94
355-361
1983
-
-
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2
-
-
-
3
-
1
-
-
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1
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1
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8
-
2
1
1
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2
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3
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1
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1
1
8
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2
1
1
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-
637535
White
Structural analogs of 5'-methy ...
Homo sapiens
Biochem. Pharmacol.
31
503-507
1982
-
-
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-
3
3
-
-
-
-
-
2
-
-
1
-
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3
-
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1
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1
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2
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2
3
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1
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3
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1
-
1
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-
-
637536
Shugart
Kinetic studies of Drosophila ...
Drosophila melanogaster
Int. J. Biochem.
13
559-564
1981
-
-
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2
-
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2
-
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1
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1
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-
-
-
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-
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-
637537
Savarese
5'-Methylthioadenosine phospho ...
Mus musculus
Biochem. Pharmacol.
30
189-199
1981
-
-
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-
1
2
-
-
-
-
-
1
-
-
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1
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1
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3
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-
-
-
637527
Carteni'-Farina
5'-Methylthioadenosine phospho ...
Caldariella acidophila
Eur. J. Biochem.
101
317-324
1979
-
-
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-
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-
1
2
-
-
-
2
-
3
-
-
1
1
-
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1
-
7
-
2
1
1
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2
1
-
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1
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2
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2
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1
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1
-
7
-
2
1
1
-
2
1
-
-
-
-
-
-
-
-
637538
Shugart
Methylthioadenosine nucleoside ...
Drosophila melanogaster
Int. J. Biochem.
10
901-904
1979
-
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2
-
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-
-
-
2
-
-
1
-
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1
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2
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1
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-
1
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-
-
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-
-
-
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-
-
-
-
637541
Ferro
5-Methylthioribose 1-phosphate ...
Rattus norvegicus
Biochim. Biophys. Acta
570
65-73
1979
-
-
-
-
-
-
6
2
-
-
1
-
-
2
-
-
1
-
-
2
2
-
3
-
1
-
-
-
1
-
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6
-
2
-
-
1
-
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1
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2
2
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
637528
Garbers
Demonstration of 5'-methylthio ...
Rattus norvegicus
Biochim. Biophys. Acta
523
82-93
1978
1
-
-
-
-
-
7
-
1
1
-
-
-
2
-
-
1
1
-
8
1
1
1
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
7
-
-
1
1
-
-
-
-
-
1
-
8
1
1
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
637540
Zappia
Substrate specificity of 5'-me ...
Homo sapiens
Biochem. J.
175
1043-1050
1978
2
-
-
-
-
1
5
1
-
-
-
-
-
2
1
-
1
1
-
2
1
-
6
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
5
-
1
-
-
-
-
-
1
-
1
-
2
1
-
6
-
-
-
1
-
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-
-
-
-
-
-
-
-
-
637526
Pegg
Phosphate-stimulated breakdown ...
Rattus norvegicus
Biochem. J.
115
241-247
1969
-
-
-
-
-
-
-
2
1
1
-
-
-
1
-
-
1
1
-
1
1
1
1
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
2
1
1
-
-
-
-
-
1
-
1
1
1
1
-
1
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-
1
1
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-
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