Literature summary for 2.4.2.3 extracted from

Larson, E.T.; Mudeppa, D.G.; Gillespie, J.R.; Mueller, N.; Napuli, A.J.; Arif, J.A.; Ross, J.; Arakaki, T.L.; Lauricella, A.; Detitta, G.; Luft, J.; Zucker, F.; Verlinde, C.L.; Fan, E.; Van Voorhis, W.C.; Buckner, F.S.; Rathod, P.K.; Hol, W.G.; Merritt, E.A.
The crystal structure and activity of a putative trypanosomal nucleoside phosphorylase reveal it to be a homodimeric uridine phosphorylase (2010), J. Mol. Biol., 396, 1244-1259.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
free selenomethionine-labeled enzyme, or enzyme in complex with uridine, X-ray diffraction structure determination and analysis at 2.7 A and 1.44 A resolution, respectively	Trypanosoma brucei

Protein Variants

Protein Variants	Comment	Organism
additional information	90% suppression of enzyme expression by RNAi does not lead to growth inhibition	Trypanosoma brucei

General Stability

General Stability	Organism
Ca2+ or EGTA stabilize the enzyme	Trypanosoma brucei

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	stabilizes the enzyme, which possesses an intermolecular metal-binding site, structure, overview	Trypanosoma brucei

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
uridine + phosphate	Trypanosoma brucei	-	uracil + alpha-D-ribose 1-phosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Trypanosoma brucei	Q57VZ2	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	pH dependence of enzyme activities with different substrates, overview	Trypanosoma brucei
1.44	-	substrate deoxyuridine, pH 6.5	Trypanosoma brucei
2.75	-	substrate uridine, pH 7.5	Trypanosoma brucei

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
deoxyuridine + phosphate	14% of the activity with uridine	Trypanosoma brucei	uracil + 2-deoxy-alpha-D-ribose 1-phosphate	-	r
guanosine + phosphate	10% of the activity with uridine	Trypanosoma brucei	guanine + alpha-D-ribose 1-phosphate	-	r
additional information	strict substrate specificty of the enzyme, overview. Deoxyadenosine, and deoxyguanosine are poor substrates, thymidine, cytidine, deoxycytidine, inosine, and adenosine are no substrates	Trypanosoma brucei	?	-	?
uridine + phosphate	-	Trypanosoma brucei	uracil + alpha-D-ribose 1-phosphate	-	r
uridine + phosphate	best substrate, active site structure, overview	Trypanosoma brucei	uracil + alpha-D-ribose 1-phosphate	-	r

Subunits

Subunits	Comment	Organism
homodimer	each monomer is composed of a central 11-stranded mixed beta-sheet surrounded by 14 alpha-helices and two peripheral short, 2-stranded antiparallel beta-sheets	Trypanosoma brucei

Synonyms

Synonyms	Comment	Organism
More	the enzyme belongs to the NP1 subfamily of the nucleoside phosphorylase, NP, superfamily of enzymes	Trypanosoma brucei
uridine phosphorylase	-	Trypanosoma brucei

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
55	-	inactivation after 50 min in presence of 2 mM EGTA, 60% of maximal activity remaining after 90 min in presence of Ca2+	Trypanosoma brucei

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	with substrate deoxyuridine	Trypanosoma brucei
7.5	-	with substrate uridine	Trypanosoma brucei

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	8.5	pH dependence of enzyme activities with different substrates, overview	Trypanosoma brucei

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Release 2023.1 (January 2023)