Literature summary for 2.4.2.3 extracted from

Prokofev, I.I.; Lashkov, A.A.; Gabdulkhakov, A.G.; Dontsova, M.V.; Seregina, T.A.; Mironov, A.S.; Betzel, C.; Mikhailov, A.M.
Crystallization and preliminary X-ray study of Vibrio cholerae uridine phosphorylase in complex with 6-methyluracil (2014), Acta Crystallogr. Sect. F, 70, 60-63.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli strain AM201	Vibrio cholerae

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with 6-methyluracil, hanging drop vapor diffusion method, using 15% (w/v) PEG 4000, 0.1 M MgCl2, 0.1 M Tris-HCl pH 8.5	Vibrio cholerae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
27500	-	6 * 27500, calculated from amino acid sequence	Vibrio cholerae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
uridine + phosphate	Vibrio cholerae	-	uracil + alpha-D-ribose 1-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Vibrio cholerae	Q9K4U1	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation, butyl Sepharose column chromatography, and Q Sepharose column chromatography	Vibrio cholerae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
6-methyluridine + phosphate	-	Vibrio cholerae	6-methyluracil + alpha-D-ribose 1-phosphate	-	?
uridine + phosphate	-	Vibrio cholerae	uracil + alpha-D-ribose 1-phosphate	-	?

Subunits

Subunits	Comment	Organism
homohexamer	6 * 27500, calculated from amino acid sequence	Vibrio cholerae

Synonyms

Synonyms	Comment	Organism
UPH	-	Vibrio cholerae

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Release 2023.1 (January 2023)