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Literature summary for 2.4.2.31 extracted from

  • Sakurai, J.; Nagahama, M.; Hisatsune, J.; Katunuma, N.; Tsuge, H.
    Clostridium perfringens i-toxin, ADP-ribosyltransferase: structure and mechanism of action (2003), Adv. Enzyme Regul., 43, 361-377.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
structure of the Ia complex with NADH at 1.8 A. Vapor diffusion method Clostridium perfringens

Protein Variants

Protein Variants Comment Organism
E301A no NADase activity detected Clostridium perfringens
F349A the ratio of turnover-number to Km-value in the NADase reaction is 1% of the wild-type ratio. The ratio of turnover-number to Km-value in the ADP-ribosyltransferase activity is 1.9% of the wild-type ratio with rabbit muscle actin as substrate and 4% of the wild-type ratio with non-muscle actin from human platelets as substrate Clostridium perfringens
F349Y the ratio of turnover-number to Km-value in the NADase reaction is 110% of the wild-type ratio Clostridium perfringens
N255A the ratio of turnover-number to Km-value in the NADase reaction is 90% of the wild-type ratio. The ratio of turnover-number to Km-value in the ADP-ribosyltransferase activity is 20% of the wild-type ratio with rabbit muscle actin as substrate and 77% of the wild-type ratio with non-muscle actin from human platelets as substrate Clostridium perfringens
R352A no ADP-ribosyltransferase activity with rabbit muscle actin and non-muscle actin from human platelets Clostridium perfringens
R352A the ratio of turnover-number to Km-value in the NADase reaction is 2% of the wild-type ratio, no activity Clostridium perfringens
Y246A the ratio of turnover-number to Km-value in the NADase reaction is 90% of the wild-type ratio. The ratio of turnover-number to Km-value in the ADP-ribosyltransferase activity is 1.9% of the wild-type ratio with rabbit muscle actin as substrate and 19% of the wild-type ratio with non-muscle actin from human platelets as substrate Clostridium perfringens
Y251A the ratio of turnover-number to Km-value in the NADase reaction is 10% of the wild-type ratio Clostridium perfringens
Y251F the ratio of turnover-number to Km-value in the NADase reaction is 40% of the wild-type ratio Clostridium perfringens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
NAD+ ADP-ribosyltransferase activity, mutant enzyme F349A, cosubstrate: non-muscle actin from human platelets Clostridium perfringens
0.58
-
NAD+ ADP-ribosyltransferase activity, mutant enzyme N255A, cosubstrate: non-muscle actin from human platelets Clostridium perfringens
0.69
-
NAD+ ADP-ribosyltransferase activity, mutant enzyme F349A, cosubstrate: rabbit muscle actin Clostridium perfringens
0.77
-
NAD+ ADP-ribosyltransferase activity, wild-type enzyme, cosubstrate: non-muscle actin from human platelets Clostridium perfringens
0.92
-
NAD+ ADP-ribosyltransferase activity, mutant enzyme Y246A, cosubstrate: non-muscle actin from human platelets Clostridium perfringens
0.93
-
NAD+ ADP-ribosyltransferase activity, mutant enzyme Y246A, cosubstrate: rabbit muscle actin Clostridium perfringens
1.28
-
NAD+ ADP-ribosyltransferase activity, wild-type enzyme, cosubstrate: rabbit muscle actin Clostridium perfringens
4.48
-
NAD+ ADP-ribosyltransferase activity, mutant enzyme N255A, cosubstrate: rabbit muscle actin Clostridium perfringens
8.67
-
NAD+ ADP-ribosyltransferase activity, mutant enzyme Y251A, cosubstrate: non-muscle actin from human platelets Clostridium perfringens

Organism

Organism UniProt Comment Textmining
Clostridium perfringens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD+ + non-muscle actin from human platelets
-
Clostridium perfringens nicotinamide + (ADP-D-ribosyl)-non-muscle actin from human platelets
-
?
NAD+ + rabbit muscle actin
-
Clostridium perfringens nicotinamide + (ADP-D-ribosyl)-rabbit muscle actin
-
?

Synonyms

Synonyms Comment Organism
ARTase
-
Clostridium perfringens
i-toxin
-
Clostridium perfringens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000067
-
NAD+ ADP-ribosyltransferase activity, mutant enzyme F349A, cosubstrate: non-muscle actin from human platelets Clostridium perfringens
0.00012
-
NAD+ ADP-ribosyltransferase activity, mutant enzyme F349A, cosubstrate: rabbit muscle actin Clostridium perfringens
0.00015
-
NAD+ ADP-ribosyltransferase activity, mutant enzyme Y246A, cosubstrate: rabbit muscle actin Clostridium perfringens
0.00133
-
NAD+ ADP-ribosyltransferase activity, mutant enzyme Y246A, cosubstrate: non-muscle actin from human platelets Clostridium perfringens
0.00133
-
NAD+ ADP-ribosyltransferase activity, mutant enzyme Y251A, cosubstrate: non-muscle actin from human platelets Clostridium perfringens
0.00183
-
NAD+ ADP-ribosyltransferase activity, mutant enzyme N255A, cosubstrate: non-muscle actin from human platelets Clostridium perfringens
0.0062
-
NAD+ ADP-ribosyltransferase activity, wild-type enzyme, cosubstrate: non-muscle actin from human platelets Clostridium perfringens
0.01
-
NAD+ ADP-ribosyltransferase activity, wild-type enzyme, cosubstrate: rabbit muscle actin Clostridium perfringens
0.014
-
NAD+ ADP-ribosyltransferase activity, mutant enzyme N255A, cosubstrate: rabbit muscle actin Clostridium perfringens