Application | Comment | Organism |
---|---|---|
drug development | more potent therapeutics for treatment of bacterial diseases and infections through understanding of ADPRT reaction meachanism | Pseudomonas aeruginosa |
Crystallization (Comment) | Organism |
---|---|
in complex with NAD+ and eEF2, PDB: 3B8H (mutant E546A), 3B82 (mutant E546H), 3B78 (mutant R551H), 2ZIT (wild-type), specific interactions of active-site loop1 with NAD+ and diphthamide results in solvent cover for dinucleotide-binding pocket and coordinates and stabilizes NAD+ in the active-site cleft during ADPRT reaction (transition-state model), crystals are of space group P(1)2(1), C2 symmetry, unit cell parameters: a: 326.9-329.4, b: 68.1-69.2, c: 190.0-191.6, beta: 102.9-103.3°, precipitant: PEG-8K or PEG-10K, 6-8%, 1.25 mM NAD+ (in cryo-protection buffer, pH 6.0) soaked into crystals | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
D461A | part of active-site loop 1 (453-463) | Pseudomonas aeruginosa |
D463A | part of active-site loop 1 (453-463) | Pseudomonas aeruginosa |
E546A | reduced ADPRT activity compared to wild-type possibly due to leakage of aqueous solvent into binding pocket which prevents ADP-ribose transfer, no impaired NAD+ binding and glycohydrolase activity, Glu546 crucial for ADPRT activity of ExoA but not strictly conserved among toxin family members, part of active-site loop 3 (546-551), upon NAD+-binding Glu546 and Tyr481 (both connected by hydrogen bonds resulting in water molecule exclusion) in hydrogen bonding distance to nucleophilic N3 of diphthamide imidazole possibly increasing its nucleophilic character | Pseudomonas aeruginosa |
E546A/R551A | double alanine mutant, almost complete loss of ADPRT activity not restored by mutations E546D/R551K or E546R/R551E | Pseudomonas aeruginosa |
E546D | lesser ADPRT activity than E546A | Pseudomonas aeruginosa |
E546F | part of active-site loop 3 (546-551) | Pseudomonas aeruginosa |
E546H | see E546A | Pseudomonas aeruginosa |
E546N | partial rescue of ADPRT activity compared to E546A | Pseudomonas aeruginosa |
E546Q | part of active-site loop 3 (546-551) | Pseudomonas aeruginosa |
E547A | part of active-site loop 3 (546-551) | Pseudomonas aeruginosa |
E548A | part of active-site loop 3 (546-551) | Pseudomonas aeruginosa |
E553A | impaired NAD+ binding and glycohydrolase activity, Glu553 is a crucial catalytic residue and conserved among toxin family members | Pseudomonas aeruginosa |
G453A | part of active-site loop 1 (453-463) | Pseudomonas aeruginosa |
G454A | part of active-site loop 1 (453-463) | Pseudomonas aeruginosa |
G454A | reduced ADPRT activity compared to wild-type, Gly454 is part of active-site loop 1 (453-463) | Pseudomonas aeruginosa |
G549A | part of active-site loop 3 (546-551) | Pseudomonas aeruginosa |
G550A | part of active-site loop 3 (546-551) | Pseudomonas aeruginosa |
L462A | part of active-site loop 1 (453-463) | Pseudomonas aeruginosa |
L552A | QuickChange mutagenesis | Pseudomonas aeruginosa |
Q460A | reduced ADPRT activity compared to wild-type, Gln460 is part of active-site loop 1 (453-463), active-site loop1 flips towards diphthamide of eEF2 by a hinged action of Ala457 and Ala464 upon NAD+ binding which places Gln460 close to adenine phosphate of NAD+, interaction of Gln460 with A-phosphate of NAD+ possibly prevents hydrolysis of NAD+ to AMP or ADP | Pseudomonas aeruginosa |
R456A | part of active-site loop 1 (453-463) | Pseudomonas aeruginosa |
R458A | 56-fold reduction in ADPRT activity, 53-fold reduction in GH activity, and 31-fold increased KD for NAD+ compared to wild-type, ADPRT activity sensitivity towards substitution in order Gln>Lys>Trp>Ala>His, Arg458 is part of active-site loop 1 (453-463) and implicated in NAD+ substrate docking and orientation, active-site loop1 flips towards diphthamide of eEF2 by a hinged action of Ala457 and Ala464 upon NAD+ binding which enables van der Waals interactions between Arg458 and the adenine base of NAD+ | Pseudomonas aeruginosa |
R458H | part of active-site loop 1 (453-463) | Pseudomonas aeruginosa |
R458K | part of active-site loop 1 (453-463) | Pseudomonas aeruginosa |
R458Q | part of active-site loop 1 (453-463) | Pseudomonas aeruginosa |
R458W | part of active-site loop 1 (453-463) | Pseudomonas aeruginosa |
R551A | ADPRT activity sensitive to replacements in order Ala>Lys>Gln>Glu>His>Cys, Arg551 is part of active-site loop 3 (546-551) | Pseudomonas aeruginosa |
R551C | part of active-site loop 3 (546-551) | Pseudomonas aeruginosa |
R551E | part of active-site loop 3 (546-551) | Pseudomonas aeruginosa |
R551H | part of active-site loop 3 (546-551) | Pseudomonas aeruginosa |
R551K | part of active-site loop 3 (546-551) | Pseudomonas aeruginosa |
R551Q | part of active-site loop 3 (546-551) | Pseudomonas aeruginosa |
S459A | part of active-site loop 1 (453-463) | Pseudomonas aeruginosa |
V455A | part of active-site loop 1 (453-463) | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
NAD+ | double mutant E546A/R551A, relative KD = 1.11 +/-0.03 | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | double mutant E546D/R551K, relative KD = 2.71 +/-0.74 | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | double mutant E546R/R551E, relative KD = 1.29 +/-0.49 | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant D461A, KD = 1.03 +/-0.02 relative to wild-type (c) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant D463A, KD = 1.18 +/-0.04 relative to wild-type (c) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant E546A, relative KD = 1.77 +/-0.34 | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant E546D, relative KD = 3.83 +/-0.89 | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant E546F, relative KD = 1.97 +/-0.11 | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant E546H, relative KD = 1.03 +/-0.09 | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant E546N, relative KD = 2.14 +/-0.26 | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant E546Q, relative KD = 2.31 +/-0.71 | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant E547A, relative KD = 1.97 +/-0.77 | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant E548A, relative KD = 2.69 +/-0.11 | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant E553A, relative KD = 2.57 +/-0.86 | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant G453A, KD = 4.85 +/-1.07 relative to wild-type (c) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant G454A, KD = 6.25 +/-0.22 relative to wild-type (c) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant G549A, relative KD = 8.00 +/-0.94 | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant G550A, relative KD = 6.60 +/-0.54 | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant L462A, KD = 1.86 +/-0.10 relative to wild-type (c) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant L552A, relative KD = 4.43 +/-0.57 | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant Q460A, KD = 4.30 +/-0.43 relative to wild-type (c) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant R456A, KD = 3.56 +/-0.53 relative to wild-type (c) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant R458A, KD = 5.78 +/-0.18 relative to wild-type (c) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant R458H, KD = 31.02 +/-7.18 relative to wild-type (d) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant R458K, KD = 3.18 +/-0.23 relative to wild-type (c) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant R458Q, KD = 0.45 +/-0.06 relative to wild-type (e) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant R458W, KD = 11.26 +/-1.16 relative to wild-type (d) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant R551A, relative KD = 2.69 +/-0.03 | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant R551C, relative KD = 5.57 +/-0.91 | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant R551E, relative KD = 5.03 +/-0.51 | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant R551H, relative KD = 2.29 +/-0.46 | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant R551K, relative KD = 1.29 +/-0.03 | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant R551Q, relative KD = 2.63 +/-0.39 | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant S459A, KD = 1.59 +/-0.16 relative to wild-type (c) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant V455A, KD = 3.31 +/-0.24 relative to wild-type (c) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | wild-type, KD = 35 +/-3 microM, relative KD = 1.00 +/-0.09, estimated according to quenched intrinsic tryptophan fluorescence upon NAD+ binding to the active site | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | wild-type, KD = 36 +/-8 microM (c), KD = 62 +/-2 microM (d), KD = 74 +/-16 microM, relative KD = 1.00 +/-0.03 | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudomonas aeruginosa | NAD+-dependent ADP-ribosyltransfer (ADPRT) including NAD+-glycohydrolysis (GH) activity, deadly virulent due to covalent modification and thus inactivation of proteins that are essential for the host | ? | - |
? | |
NAD+ + essential ribosomal elongation factor 2 | Pseudomonas aeruginosa | transfer of ADP-ribose moiety (oxocarbenium ion) of NAD+ onto N3 of diphthamide imidazole of essential ribosomal elongation factor 2 (eEF2) | nicotinamide + H+ + ADP-ribosylated essential ribosomal elongation factor 2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | P11439 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | NAD+-dependent ADP-ribosyltransfer (ADPRT) including NAD+-glycohydrolysis (GH) activity, deadly virulent due to covalent modification and thus inactivation of proteins that are essential for the host | Pseudomonas aeruginosa | ? | - |
? | |
additional information | no ADP-ribosyl transfer by mutants R551H and R551C despite of occurring NAD+-binding and hydrolysis | Pseudomonas aeruginosa | ? | - |
? | |
NAD+ + essential ribosomal elongation factor 2 | transfer of ADP-ribose moiety (oxocarbenium ion) of NAD+ onto N3 of diphthamide imidazole of essential ribosomal elongation factor 2 (eEF2) | Pseudomonas aeruginosa | nicotinamide + H+ + ADP-ribosylated essential ribosomal elongation factor 2 | - |
? | |
NAD+ + essential ribosomal elongation factor 2 | essential ribosomal elongation factor 2: eEF2, 2-step reaction by concerted GH and ADPRT activity, transition-state model | Pseudomonas aeruginosa | nicotinamide + H+ + ADP-ribosylated essential ribosomal elongation factor 2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ExoA | - |
Pseudomonas aeruginosa |
ExoA(c) | - |
Pseudomonas aeruginosa |
exotoxin A | - |
Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
NAD+ | 0.0062-0.13/min, wild-type GH activity, in the absence of eEF2, pH 7.9, 25°C, 60 min | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | 0.125 +/-0.007/min (a), wild-type GH activity, in the absence of eEF2, pH 7.9, 25°C, 60 min | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | 0.130 +/-0.012/min (b), wild-type GH activity, in the absence of eEF2, pH 7.9, 25°C, 60 min | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | 1306 +/-121/min (a), wild-type ADPRT activity, 12 microM eEF2, pH 7.9, 25°C, 300 sec | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | 1495 +/-224/min (b), wild-type ADPRT activity, 12 microM eEF2, pH 7.9, 25°C, 300 sec | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | 628 +/-8/min (B), wild-type ADPRT activity, 12 microM eEF2, pH 7.9, 25°C, 300 sec | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | 746 +/-18/min (A), wild-type ADPRT activity, 12 microM eEF2, pH 7.9, 25°C, 300 sec | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | 847 +/-21/min (C), wild-type ADPRT activity, 12 microM eEF2, pH 7.9, 25°C, 300 sec | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | 900 +/-20/min (D), wild-type ADPRT activity, 12 microM eEF2, pH 7.9, 25°C, 300 sec | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | double mutant E546A/R551A, kcat = 0.0001 +/-0.0001, relative to wild-type ADPRT activity (C) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | double mutant E546A/R551A, kcat = 0.84 +/-0.04, relative to wild-type GH activity | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | double mutant E546D/R551K, kcat = 0.0009 +/-0.0013, relative to wild-type ADPRT activity (C) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | double mutant E546D/R551K, kcat = 0.42 +/-0.0002, relative to wild-type GH activity | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | double mutant E546R/R551E, kcat = 0.00001 +/-0.000002, relative to wild-type ADPRT activity (C) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | double mutant E546R/R551E, kcat = 0.38 +/-0.01, relative to wild-type GH activity | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant D461A, kcat = 1.001 +/-0.091, relative to wild-type ADPRT activity (a) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant D461A, kcat = 1.076 +/-0.041, relative to wild-type GH activity (a) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant D463A, kcat = 1.148 +/-0.038, relative to wild-type GH activity (a) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant D463A, kcat = 1.270 +/-0.204, relative to wild-type ADPRT activity (a) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant E546A, kcat = 0.0012 +/-0.00002, relative to wild-type ADPRT activity (A) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant E546A, kcat = 0.24 +/-0.008, relative to wild-type GH activity | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant E546D, kcat = 0.00025 +/-0.00005, relative to wild-type ADPRT activity (B) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant E546D, kcat = 0.45 +/-0.002, relative to wild-type GH activity | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant E546F, kcat = 0.007 +/-0.0002, relative to wild-type ADPRT activity (D) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant E546F, kcat = 0.69 +/-0.05, relative to wild-type GH activity | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant E546H, kcat = 0.0012 +/-0.0002, relative to wild-type ADPRT activity (B) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant E546H, kcat = 0.59 +/-0.009, relative to wild-type GH activity | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant E546N, kcat = 0.0010 +/-0.00009, relative to wild-type ADPRT activity (B) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant E546N, kcat = 0.67 +/-0.03, relative to wild-type GH activity | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant E546Q, kcat = 0.011 +/-0.002, relative to wild-type ADPRT activity (B) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant E546Q, kcat = 0.80 +/-0.04, relative to wild-type GH activity | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant E547A, kcat = 0.795 +/-0.077, relative to wild-type ADPRT activity (A) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant E547A, kcat = 0.83 +/-0.14, relative to wild-type GH activity | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant E548A, kcat = 0.76 +/-0.01, relative to wild-type GH activity | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant E548A, kcat = 1.669 +/-0.298, relative to wild-type ADPRT activity (A) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant E553A, kcat = 0.0015 +/-0.00005, relative to wild-type ADPRT activity (A) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant E553A, kcat = 0.07 +/-0.009, relative to wild-type GH activity | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant G453A, kcat = 0.290 +/-0.040, relative to wild-type ADPRT activity (a) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant G453A, kcat = 0.331 +/-0.019, relative to wild-type GH activity (a) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant G454A, kcat = 0.031 +/-0.003, relative to wild-type GH activity (a) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant G454A, kcat = 0.046 +/-0.008, relative to wild-type ADPRT activity (a) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant G549A, kcat = 0.30 +/-0.005, relative to wild-type GH activity | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant G549A, kcat = 0.770 +/-0.076, relative to wild-type ADPRT activity (A) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant G550A, kcat = 0.41 +/-0.04, relative to wild-type GH activity | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant G550A, kcat = 0.562 +/-0.166, relative to wild-type ADPRT activity (A) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant L462A, kcat = 0.268 +/-0.041, relative to wild-type ADPRT activity (a) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant L462A, kcat = 0.955 +/-0.015, relative to wild-type GH activity (a) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant L552A, kcat = 0.58 +/-0.002, relative to wild-type GH activity | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant L552A, kcat = 2.365 +/-0.527, relative to wild-type ADPRT activity (A) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant Q460A, kcat = 0.115 +/-0.010, relative to wild-type ADPRT activity (a) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant Q460A, kcat = 0.622 +/-0.014, relative to wild-type GH activity (a) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant R456A, kcat = 0.230 +/-0.010, relative to wild-type GH activity (a) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant R456A, kcat = 0.239 +/-0.034, relative to wild-type ADPRT activity (a) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant R458A, kcat = 0.132 +/-0.031, relative to wild-type ADPRT activity (a) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant R458A, kcat = 0.249 +/-0.021, relative to wild-type GH activity (a) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant R458H, kcat = 0.018 +/-0.003, relative to wild-type GH activity (b) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant R458H, kcat = 0.019 +/-0.007, relative to wild-type ADPRT activity (b) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant R458K, kcat = 0.428 +/-0.033, relative to wild-type GH activity (a) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant R458K, kcat = 0.543 +/-0.155, relative to wild-type ADPRT activity (a) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant R458Q, kcat = 0.650 +/-0.130, relative to wild-type ADPRT activity (b) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant R458Q, kcat = 0.991 +/-0.017, relative to wild-type GH activity (b) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant R458W, kcat = 0.379 +/-0.043, relative to wild-type ADPRT activity (b) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant R458W, kcat = 0.438 +/-0.040, relative to wild-type GH activity (b) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant R551A, kcat = 0.32 +/-0.005, relative to wild-type GH activity | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant R551A, kcat = 0.380 +/-0.024, relative to wild-type ADPRT activity (A) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant R551C, kcat = ~0, relative to wild-type ADPRT activity (B) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant R551C, kcat = 0.10 +/-0.006, relative to wild-type GH activity | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant R551E, kcat = 0.011 +/-0.005, relative to wild-type ADPRT activity (B) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant R551E, kcat = 0.09 +/-0.001, relative to wild-type GH activity | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant R551H, kcat = ~0, relative to wild-type ADPRT activity (B) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant R551H, kcat = 0.41 +/-0.002, relative to wild-type GH activity | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant R551K, kcat = 0.317 +/-0.024, relative to wild-type ADPRT activity (B) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant R551K, kcat = 0.69 +/-0.03, relative to wild-type GH activity | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant R551Q, kcat = 0.185 +/-0.014, relative to wild-type ADPRT activity (B) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant R551Q, kcat = 0.38 +/-0.01, relative to wild-type GH activity | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant S459A, kcat = 1.119 +/-0.054, relative to wild-type GH activity (a) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant S459A, kcat = 1.256 +/-0.144, relative to wild-type ADPRT activity (a) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant V455A, kcat = 0.241 +/-0.032, relative to wild-type ADPRT activity (a) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | mutant V455A, kcat = 0.254 +/-0.001, relative to wild-type GH activity (a) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | relative kcat = 1.00 +/-0.02, wild-type GH activity | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | relative kcat = 1.00 +/-0.05, wild-type GH activity (a) and (b) | Pseudomonas aeruginosa | |
additional information | - |
NAD+ | relative kcat = 1.00 +/-0.09, wild-type ADPRT activity (A)-(D), and (a) and (b) | Pseudomonas aeruginosa |