Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | strong stimulation even in the presence of Mg2+ | Euonymus alatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10 mM, 75% inhibition | Euonymus alatus | |
Co2+ | 1 mM, 96% inhibition | Euonymus alatus | |
Cu2+ | 1 mM, 99% inhibition | Euonymus alatus | |
iodoacetamide | 1 mM, 44% inhibition | Euonymus alatus | |
Mg2+ | 20 mM, 77% inhibition | Euonymus alatus | |
Mn2+ | 10 mM, 98% inhibition | Euonymus alatus | |
Phenylmercuriacetate | 1 mM, 98% inhibition | Euonymus alatus | |
Zn2+ | 1 mM, 99% inhibition | Euonymus alatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00083 | - |
kaempferol | pH 7.5, 30°C | Euonymus alatus | |
0.00119 | - |
quercetin | pH 7.5, 30°C | Euonymus alatus | |
0.025 | - |
UDP-alpha-D-xylose | pH 7.5, 30°C, cosubstrate: kaempferol | Euonymus alatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
gel filtration | Euonymus alatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-xylose + kaempferol | Euonymus alatus | 3-O-xylosylation of flavonols | UDP + kaempferol 3-O-beta-D-xyloside | - |
? | |
UDP-alpha-D-xylose + kaempferol | Euonymus alatus Thunb. Siebold f. ciliatodentatus | 3-O-xylosylation of flavonols | UDP + kaempferol 3-O-beta-D-xyloside | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Euonymus alatus | - |
- |
- |
Euonymus alatus Thunb. Siebold f. ciliatodentatus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Euonymus alatus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | young | Euonymus alatus | - |
Storage Stability | Organism |
---|---|
-20°C, 20 mM Tris-HCl buffer, pH 7.5, 14 mM 2-mercaptoethanol and 10% glycerol, 11% loss of activity after 2 days, 55% loss after 8 days, 84% loss after 40 days | Euonymus alatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-xylose + 3,3',4',7-tetrahydroxy-5'-methoxyflavone | 20% of the activity compared to kaempferol | Euonymus alatus | UDP + 3,3',4',7-tetrahydroxy-5'-methoxyflavone 3-O-beta-D-xyloside | - |
? | |
UDP-alpha-D-xylose + 3,3',4',7-tetrahydroxy-5'-methoxyflavone | 20% of the activity compared to kaempferol | Euonymus alatus Thunb. Siebold f. ciliatodentatus | UDP + 3,3',4',7-tetrahydroxy-5'-methoxyflavone 3-O-beta-D-xyloside | - |
? | |
UDP-alpha-D-xylose + 3,4',7-trihydroxyflavone | 32% of the activity compared to kaempferol | Euonymus alatus | UDP + 3,4',7-trihydroxyflavone 3-O-beta-D-xyloside | - |
? | |
UDP-alpha-D-xylose + 3,4',7-trihydroxyflavone | 32% of the activity compared to kaempferol | Euonymus alatus Thunb. Siebold f. ciliatodentatus | UDP + 3,4',7-trihydroxyflavone 3-O-beta-D-xyloside | - |
? | |
UDP-alpha-D-xylose + 3,5,7-trihydroxy-4'-methoxyflavone | 45% of the activity compared to kaempferol | Euonymus alatus | UDP + 3,5,7-trihydroxy-4'-methoxyflavone 3-O-beta-D-xyloside | - |
? | |
UDP-alpha-D-xylose + 3,5,7-trihydroxy-4'-methoxyflavone | 45% of the activity compared to kaempferol | Euonymus alatus Thunb. Siebold f. ciliatodentatus | UDP + 3,5,7-trihydroxy-4'-methoxyflavone 3-O-beta-D-xyloside | - |
? | |
UDP-alpha-D-xylose + 3-hydroxy-4',5,7-trimethoxyflavone | 15% of the activity compared to kaempferol | Euonymus alatus | UDP + 3-hydroxy-4',5,7-trimethoxyflavone 3-O-beta-D-xyloside | - |
? | |
UDP-alpha-D-xylose + 4',5,7-trihydroxyflavan-3-ol | 25% of the activity compared to kaempferol | Euonymus alatus | UDP + 3-O-beta-D-xylopyranosyl-4',5,7-trihydroxyflavan-3-ol | - |
? | |
UDP-alpha-D-xylose + fisetin | 62% of the activity compared to kaempferol | Euonymus alatus | UDP + fisetin 3-O-beta-D-xyloside | - |
? | |
UDP-alpha-D-xylose + isorhamnetin | 85% of the activity compared to kaempferol | Euonymus alatus | UDP + isorhamnetin 3-O-beta-D-xyloside | - |
? | |
UDP-alpha-D-xylose + kaempferol | best substrate | Euonymus alatus | UDP + kaempferol 3-O-beta-D-xyloside | - |
? | |
UDP-alpha-D-xylose + kaempferol | 3-O-xylosylation of flavonols | Euonymus alatus | UDP + kaempferol 3-O-beta-D-xyloside | - |
? | |
UDP-alpha-D-xylose + kaempferol | best substrate | Euonymus alatus Thunb. Siebold f. ciliatodentatus | UDP + kaempferol 3-O-beta-D-xyloside | - |
? | |
UDP-alpha-D-xylose + kaempferol | 3-O-xylosylation of flavonols | Euonymus alatus Thunb. Siebold f. ciliatodentatus | UDP + kaempferol 3-O-beta-D-xyloside | - |
? | |
UDP-alpha-D-xylose + myricetin | 48% of the activity compared to kaempferol | Euonymus alatus | UDP + myricetin 3-O-beta-D-xyloside | - |
? | |
UDP-alpha-D-xylose + quercetin | 76% of the activity compared to kaempferol | Euonymus alatus | UDP + quercetin 3-O-beta-D-xyloside | - |
? | |
UDP-alpha-D-xylose + rhamnetin | 67% of the activity compared to kaempferol | Euonymus alatus | UDP + rhamnetin 3-O-beta-D-xyloside | - |
? |
Synonyms | Comment | Organism |
---|---|---|
F3XT | - |
Euonymus alatus |
flavonol 3-O-xylosyltransferase | - |
Euonymus alatus |
UDP-D-xylose:flavonol 3-O-xylosyltransferase | - |
Euonymus alatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
Tris-HCl buffer | Euonymus alatus |
7.5 | - |
imidazole-HCl buffer | Euonymus alatus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Euonymus alatus | isoelectric focusing | - |
6.1 |