Any feedback?
Literature summary for 2.4.2.6 extracted from
- Structure-guided tuning of a selectivity switch towards ribonucleosides in Trypanosoma brucei purine nucleoside 2-deoxyribosyltransferase (2019), ChemBioChem, 20, 2996-3000 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene pdt, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Trypanosoma brucei brucei |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| N53D | site-directed mutagenesis, the mutant shows no ribosyltransferase activity and reduced activity compared to the wild-type enzyme | Trypanosoma brucei brucei |
| Y5F | site-directed mutagenesis, the deoxyribose/ribose selectivity ratio improves about tenfold compared to wild-type if Tyr5 is replaced with a phenylalanine, the mutant shows reduced activity compared to the wild-type enzyme | Trypanosoma brucei brucei |
| Y5F/N53D | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Trypanosoma brucei brucei |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-deoxy-D-ribosyl-base1 + base2 | Trypanosoma brucei brucei | - |
2-deoxy-D-ribosyl-base2 + base1 | - |
? |  |
| 2-deoxy-D-ribosyl-base1 + base2 | Trypanosoma brucei brucei 927/4 GUTat10.1 | - |
2-deoxy-D-ribosyl-base2 + base1 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma brucei brucei | Q57VC7 | - |
- |
| Trypanosoma brucei brucei 927/4 GUTat10.1 | Q57VC7 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Trypanosoma brucei brucei |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.1 | - |
below, recombinant mutant Y5F/N53D with substrates inosine and adenine, pH 4.0, 50°C | Trypanosoma brucei brucei |
| 0.1 | - |
below, recombinant wild-type enzyme with substrates inosine and adenine, pH 4.0, 50°C | Trypanosoma brucei brucei |
| 0.2 | - |
recombinant mutant N53D with substrates inosine and adenine, pH 6.0, 50°C | Trypanosoma brucei brucei |
| 0.5 | - |
recombinant mutant Y5F with substrates inosine and adenine, pH 4.0, 50°C | Trypanosoma brucei brucei |
| 0.9 | - |
recombinant wild-type enzyme with substrates inosine and adenine, pH 6.0, 50°C | Trypanosoma brucei brucei |
| 1.6 | - |
recombinant mutant Y5F/N53D with substrates inosine and adenine, pH 6.0, 50°C | Trypanosoma brucei brucei |
| 2.7 | - |
recombinant mutant Y5F with substrates inosine and adenine, pH 6.0, 50°C | Trypanosoma brucei brucei |
| 7.3 | - |
recombinant mutant Y5F/N53D with substrates 2'-deoxyinosine and adenine, pH 6.0, 50°C | Trypanosoma brucei brucei |
| 11 | - |
recombinant mutant N53D with substrates 2'-deoxyinosine and adenine, pH 6.0, 50°C | Trypanosoma brucei brucei |
| 11.1 | - |
recombinant mutant Y5F/N53D with substrates 2'-deoxyinosine and adenine, pH 4.0, 50°C | Trypanosoma brucei brucei |
| 11.3 | - |
recombinant mutant Y5F with substrates 2'-deoxyinosine and adenine, pH 6.0, 50°C | Trypanosoma brucei brucei |
| 12.3 | - |
recombinant mutant Y5F with substrates 2'-deoxyinosine and adenine, pH 4.0, 50°C | Trypanosoma brucei brucei |
| 38.5 | - |
recombinant wild-type enzyme with substrates 2'-deoxyinosine and adenine, pH 6.0, 50°C | Trypanosoma brucei brucei |
| 47.5 | - |
recombinant mutant N53D with substrates 2'-deoxyinosine and adenine, pH 4.0, 50°C | Trypanosoma brucei brucei |
| 47.5 | - |
recombinant wild-type enzyme with substrates 2'-deoxyinosine and adenine, pH 4.0, 50°C | Trypanosoma brucei brucei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-deoxyguanosine + 2,6-diaminopurine | - |
Trypanosoma brucei brucei | 2,6-diaminopurine-2'deoxyriboside + guanine | - |
? | |
| 2'-deoxyguanosine + 2,6-diaminopurine | - |
Trypanosoma brucei brucei 927/4 GUTat10.1 | 2,6-diaminopurine-2'deoxyriboside + guanine | - |
? | |
| 2'-deoxyguanosine + 2-chloroadenine | - |
Trypanosoma brucei brucei | 2-chloro-2'-deoxyadenosine + guanine | - |
? | |
| 2'-deoxyguanosine + 2-chloroadenine | - |
Trypanosoma brucei brucei 927/4 GUTat10.1 | 2-chloro-2'-deoxyadenosine + guanine | - |
? | |
| 2'-deoxyguanosine + 2-fluoroadenine | - |
Trypanosoma brucei brucei | 2-fluoro-2'-deoxyadenosine + guanine | - |
? | |
| 2'-deoxyguanosine + 6-chloropurine | - |
Trypanosoma brucei brucei | 6-chloropurine-2'-deoxyriboside + guanine | - |
? | |
| 2'-deoxyguanosine + 6-mercaptopurine | - |
Trypanosoma brucei brucei | 6-mercaptopurine-2'-deoxyriboside + guanine | - |
? | |
| 2'-deoxyguanosine + 6-methoxyguanine | - |
Trypanosoma brucei brucei | 6-methoxy-2'-deoxyguanosine + guanine | - |
? | |
| 2'-deoxyguanosine + 6-methylpurine | - |
Trypanosoma brucei brucei | 6-methylpurine-2'-deoxyriboside + guanine | - |
? | |
| 2'-deoxyguanosine + adenine | - |
Trypanosoma brucei brucei | 2'-deoxyadenosine + guanine | - |
? | |
| 2'-deoxyguanosine + benzimidazole | - |
Trypanosoma brucei brucei | benzimidazole 2'-deoxyriboside + guanine | - |
? | |
| 2'-deoxyguanosine + hypoxanthine | - |
Trypanosoma brucei brucei | 2'-deoxyinosine + guanine | - |
? | |
| 2'-deoxyinosine + adenine | - |
Trypanosoma brucei brucei | 2'-deoxyadenosine + hypoxanthine | - |
? | |
| 2'-deoxyinosine + adenine | - |
Trypanosoma brucei brucei 927/4 GUTat10.1 | 2'-deoxyadenosine + hypoxanthine | - |
? | |
| 2-deoxy-D-ribosyl-base1 + base2 | - |
Trypanosoma brucei brucei | 2-deoxy-D-ribosyl-base2 + base1 | - |
? | |
| 2-deoxy-D-ribosyl-base1 + base2 | - |
Trypanosoma brucei brucei 927/4 GUTat10.1 | 2-deoxy-D-ribosyl-base2 + base1 | - |
? | |
| inosine + adenine | cf. EC 2.4.2.5 | Trypanosoma brucei brucei | adenosine + hypoxanthine | - |
? | |
| additional information | no activity with xanthine, 7-deazaxanthine, and 7-deazahypoxanthine, substrate specificity, overview. No significant preference exists between 6-aminopurines and 6-oxopurines as base acceptors. Binding structures of inosine within the active site of wild-type TbPDT and TbPDTY5F | Trypanosoma brucei brucei | ? | - |
- |
|
| additional information | no activity with xanthine, 7-deazaxanthine, and 7-deazahypoxanthine, substrate specificity, overview. No significant preference exists between 6-aminopurines and 6-oxopurines as base acceptors. Binding structures of inosine within the active site of wild-type TbPDT and TbPDTY5F | Trypanosoma brucei brucei 927/4 GUTat10.1 | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 19000, recombinant enzyme, SDS-PAGE | Trypanosoma brucei brucei |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| nucleoside 2'-deoxyribosyltransferase | - |
Trypanosoma brucei brucei |
| Tb927.5.1360 | locus name | Trypanosoma brucei brucei |
| TbPDT | - |
Trypanosoma brucei brucei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Trypanosoma brucei brucei |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 69 | - |
TbPDTY5F variant is a homodimer that shows a remarkably high melting temperature of 69°C | Trypanosoma brucei brucei |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Trypanosoma brucei brucei |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
