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Literature summary for 2.4.2.7 extracted from
- Purification and characterization of adenine phosphoribosyltransferase from mouse mammary carcinoma FM3A cells in culture (1986), Biochim. Biophys. Acta, 884, 304-310.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not affected by 2,6-diaminopurine, 4-carbamoylimidazolium 5-olate, 8-azaadenine, and 2-fluoro-6-aminopurine | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AMP | competitive against 5-phospho-alpha-D-ribose 1-diphosphate | Mus musculus |  |
| additional information | not affected by 2,6-diaminopurine, 4-carbamoylimidazolium 5-olate, 8-azaadenine, and 2-fluoro-6-aminopurine | Mus musculus | |
| p-chloromercuribenzoate | no inhibition | Mus musculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0012 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
Mus musculus | |
| 0.007 | - |
adenine | - |
Mus musculus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Mus musculus | |
| Mg2+ | absolute requirement | Mus musculus | |
| Mg2+ | half-maximal activation at 0.4 mM | Mus musculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
2 * 23000, SDS-PAGE | Mus musculus |
| 44000 | - |
gel filtration | Mus musculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| F28-7 cell | cell line derived from FM3A | Mus musculus | - |
| FM3A cell | mammary carcinoma cell line | Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 20.6 | - |
purified enzyme | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-carbamoylimidazolium 5-olate + 5-phospho-alpha-D-ribose 1-diphosphate | low activity | Mus musculus | 4-carbamoylimidazolium 5-olate 5'-phosphate + diphosphate | - |
? | |
| adenine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Mus musculus | AMP + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 23000, SDS-PAGE | Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Mus musculus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | active over a broad range increasing progressively in activity from pH 7.0-9.0 | Mus musculus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.024 | - |
AMP | - |
Mus musculus | |
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