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Literature summary for 2.4.2.8 extracted from
- Cloning, characterization and preliminary crystallographic analysis of Leishmania hypoxanthine-guanine phosphoribosyltransferase (2002), Biochim. Biophys. Acta, 1598, 3-9.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | potential target for antiparasitic chemotherapy | Leishmania tarentolae |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli BL21(DE3) | Leishmania tarentolae |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| recombinant enzyme, 7 mg/ml, hanging-drop vapour-diffusion method, TMD buffer, pH 7.5, + equal volume of reservoir solution: 18°C or 4°C, pH 5.6 , 19% isopropanol, 19% polyethylene glycol 4000, 5% glycerol, or 17% polyethylene glycol 4000, 5% glycerol | Leishmania tarentolae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0028 | - |
guanine | recombinant enzyme, with 5-phospho-alpha-D-ribose 1-diphosphate | Leishmania tarentolae |  |
| 0.0044 | - |
hypoxanthine | recombinant enzyme, with 5-phospho-alpha-D-ribose 1-diphosphate | Leishmania tarentolae | |
| 0.127 | - |
5-phosphoribosyl 1-diphosphate | recombinant enzyme, with guanine | Leishmania tarentolae | |
| 0.138 | - |
5-phosphoribosyl 1-diphosphate | recombinant enzyme, with hypoxanthine | Leishmania tarentolae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
2 * 23000, recombinant enzyme, SDS-PAGE | Leishmania tarentolae |
| 50000 | - |
recombinant enzyme, gel filtration | Leishmania tarentolae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Leishmania tarentolae | salvage incorporation of exogenous purine nucleotides, no de novo synthesis | ? | - |
? | |
| additional information | Leishmania tarentolae | enzyme is essential for salvaging exogenous purine bases | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leishmania tarentolae | Q9NJI5 | gene hgprt | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant from Escherichia coli | Leishmania tarentolae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Leishmania tarentolae | GMP + diphosphate | - |
? | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Leishmania tarentolae | IMP + diphosphate | - |
? | |
| additional information | salvage incorporation of exogenous purine nucleotides, no de novo synthesis | Leishmania tarentolae | ? | - |
? | |
| additional information | enzyme is essential for salvaging exogenous purine bases | Leishmania tarentolae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 23000, recombinant enzyme, SDS-PAGE | Leishmania tarentolae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
recombinant enzyme, pI: 8.2 | Leishmania tarentolae |
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