Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzyme in Escherichia coli, functional complementation study | Plasmodium falciparum |
expression of wild-type and mutant enzymes in Escherichia coli, functional complementation study | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
F36A | site-directed mutagenesis, unaltered substrate specificity compared to the wild-type enzyme | Homo sapiens |
F36E | site-directed mutagenesis, unaltered substrate specificity compared to the wild-type enzyme | Homo sapiens |
F36G | site-directed mutagenesis, inactive mutant | Homo sapiens |
F36K | site-directed mutagenesis, unaltered substrate specificity compared to the wild-type enzyme | Homo sapiens |
F36L | random mutagenesis, mutant phosphoribosylates xanthine, mutant does not bind the purine substrate directly, long-range modulation via loop IV influence the substrate specificity, altered enzyme stability | Homo sapiens |
F36W | site-directed mutagenesis, unaltered substrate specificity compared to the wild-type enzyme | Homo sapiens |
L44F | site-directed mutagenesis, mutant does not phosphoribosylate guanine and xanthine, modulation via loop IV influence the substrate specificity, altered enzyme stability | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 22°C, recombinant mutant F36L, with xanthine | Homo sapiens | |
0.001 | - |
guanine | pH 7.4, 22°C, recombinant wild-type enzyme | Plasmodium falciparum | |
0.001 | - |
hypoxanthine | pH 7.4, 22°C, recombinant wild-type enzyme and mutant L44F | Plasmodium falciparum | |
0.0014 | - |
hypoxanthine | pH 7.4, 22°C, recombinant wild-type enzyme | Homo sapiens | |
0.0016 | - |
hypoxanthine | pH 7.4, 22°C, recombinant mutant F36K | Homo sapiens | |
0.0018 | - |
hypoxanthine | pH 7.4, 22°C, recombinant mutant F36G | Homo sapiens | |
0.002 | - |
guanine | pH 7.4, 22°C, recombinant mutant F36L | Homo sapiens | |
0.002 | - |
guanine | pH 7.4, 22°C, recombinant mutant L44F | Plasmodium falciparum | |
0.0029 | - |
guanine | pH 7.4, 22°C, recombinant mutant F36K | Homo sapiens | |
0.0044 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 22°C, recombinant mutant F36K, with hypoxanthine | Homo sapiens | |
0.0044 | - |
hypoxanthine | pH 7.4, 22°C, recombinant mutant F36L | Homo sapiens | |
0.0045 | - |
guanine | pH 7.4, 22°C, recombinant wild-type enzyme | Homo sapiens | |
0.0061 | - |
guanine | pH 7.4, 22°C, recombinant mutant F36G | Homo sapiens | |
0.011 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 22°C, recombinant mutant F36G, with hypoxanthine | Homo sapiens | |
0.027 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 22°C, recombinant mutant F36L, with hypoxanthine | Homo sapiens | |
0.029 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 22°C, recombinant mutant F36K, with guanine | Homo sapiens | |
0.03 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 22°C, recombinant wild-type enzyme, with hypoxanthine | Plasmodium falciparum | |
0.035 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 22°C, recombinant wild-type enzyme, with hypoxanthine | Homo sapiens | |
0.041 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 22°C, recombinant mutant F36L, with guanine | Homo sapiens | |
0.05 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 22°C, recombinant mutant F36G, with guanine | Homo sapiens | |
0.076 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 22°C, recombinant wild-type enzyme, with guanine | Homo sapiens | |
0.079 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 22°C, recombinant wild-type enzyme, with xanthine | Plasmodium falciparum | |
0.102 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 22°C, recombinant mutant L44F, with hypoxanthine | Plasmodium falciparum | |
0.116 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 22°C, recombinant mutant L44F, with guanine | Plasmodium falciparum | |
0.151 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 22°C, recombinant mutant L44F, with xanthine | Plasmodium falciparum | |
0.201 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 22°C, recombinant wild-type enzyme, with guanine | Plasmodium falciparum | |
0.261 | - |
xanthine | pH 7.4, 22°C, recombinant wild-type enzyme | Plasmodium falciparum | |
0.287 | - |
xanthine | pH 7.4, 22°C, recombinant mutant F36L | Homo sapiens | |
0.853 | - |
xanthine | pH 7.4, 22°C, recombinant mutant L44F | Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GMP + diphosphate | Homo sapiens | - |
guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
guanine + 5-phospho-alpha-D-ribose 1-diphosphate | Plasmodium falciparum | - |
GMP + diphosphate | - |
? | |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Plasmodium falciparum | - |
IMP + diphosphate | - |
? | |
IMP + diphosphate | Homo sapiens | - |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
xanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Plasmodium falciparum | wild-type enzyme | xanthosine 5'-phosphate + diphosphate | - |
? | |
xanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Homo sapiens | mutant F36L, no activity with the wild-type enzyme | xanthosine 5'-phosphate + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P00492 | - |
- |
Plasmodium falciparum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant L44F from Escherichia coli | Plasmodium falciparum |
recombinant wild-type and mutants F36L, F36G, F36K from Escherichia coli | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | residue F36 is responsible for substrate specificity, structure-function relationship | Homo sapiens | |
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | residue L44 is responsible for substrate specificity, structure-function relationship | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GMP + diphosphate | - |
Homo sapiens | guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Plasmodium falciparum | GMP + diphosphate | - |
? | |
guanine + 5-phospho-alpha-D-ribose 1-diphosphate | wild-type enzyme, no activity with mutant F36L | Homo sapiens | GMP + diphosphate | - |
? | |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Plasmodium falciparum | IMP + diphosphate | - |
? | |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | wild-type enzyme, no activity with mutant F36L | Homo sapiens | IMP + diphosphate | - |
? | |
IMP + diphosphate | - |
Homo sapiens | hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
additional information | the flexibilty of loop IV can influence the substrate specificity | Homo sapiens | ? | - |
? | |
xanthine + 5-phospho-alpha-D-ribose 1-diphosphate | wild-type enzyme | Plasmodium falciparum | xanthosine 5'-phosphate + diphosphate | - |
? | |
xanthine + 5-phospho-alpha-D-ribose 1-diphosphate | mutant F36L, no activity with the wild-type enzyme | Homo sapiens | xanthosine 5'-phosphate + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HGPRT | - |
Plasmodium falciparum |
HGPRT | - |
Homo sapiens |
hypoxanthine guanine phosphoribosyltransferase | - |
Plasmodium falciparum |
hypoxanthine guanine phosphoribosyltransferase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Plasmodium falciparum |
22 | - |
assay at room temperature | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
3 h, wild-type and mutant F36L, over 80% remaining activity | Homo sapiens |
70 | - |
0.5 h, wild-type and mutant F36L, over 80% remaining activity | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
xanthine | pH 7.4, 22°C, recombinant mutant F36L | Homo sapiens | |
0.94 | - |
hypoxanthine | pH 7.4, 22°C, recombinant mutant L44F | Plasmodium falciparum | |
1.1 | - |
guanine | pH 7.4, 22°C, recombinant mutant L44F | Plasmodium falciparum | |
1.13 | - |
hypoxanthine | pH 7.4, 22°C, recombinant wild-type enzyme | Plasmodium falciparum | |
1.2 | - |
xanthine | pH 7.4, 22°C, recombinant mutant L44F | Plasmodium falciparum | |
1.2 | - |
guanine | pH 7.4, 22°C, recombinant wild-type enzyme | Plasmodium falciparum | |
2.9 | - |
xanthine | pH 7.4, 22°C, recombinant wild-type enzyme | Plasmodium falciparum | |
3.1 | - |
hypoxanthine | pH 7.4, 22°C, recombinant mutant F36G | Homo sapiens | |
3.4 | - |
guanine | pH 7.4, 22°C, recombinant mutant F36G | Homo sapiens | |
3.4 | - |
hypoxanthine | pH 7.4, 22°C, recombinant mutant F36K | Homo sapiens | |
5.4 | - |
hypoxanthine | pH 7.4, 22°C, recombinant mutant F36L | Homo sapiens | |
6.3 | - |
guanine | pH 7.4, 22°C, recombinant mutant F36K | Homo sapiens | |
7.1 | - |
hypoxanthine | pH 7.4, 22°C, recombinant wild-type enzyme | Homo sapiens | |
18 | - |
guanine | pH 7.4, 22°C, recombinant mutant F36L | Homo sapiens | |
25.5 | - |
guanine | pH 7.4, 22°C, recombinant wild-type enzyme | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Plasmodium falciparum |
7.4 | - |
assay at | Homo sapiens |