KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0065 | - |
5-phospho-alpha-D-ribose 1-diphosphate | with guanine as cosubstrate, at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
0.01 | - |
guanine | at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
0.0141 | - |
5-phospho-alpha-D-ribose 1-diphosphate | with hypoxanthine as cosubstrate, at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
0.026 | - |
hypoxanthine | at pH 7.5 and 25°C | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22251 | - |
2 * 22251, calculated from amino acid sequence | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanine + 5-phospho-alpha-D-ribose 1-diphosphate | Mycobacterium tuberculosis | - |
GMP + diphosphate | - |
? | |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Mycobacterium tuberculosis | - |
IMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WHQ9 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Mycobacterium tuberculosis | GMP + diphosphate | - |
? | |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Mycobacterium tuberculosis | IMP + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 22251, calculated from amino acid sequence | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
HGPRT | - |
Mycobacterium tuberculosis |
hypoxanthine-guanine phosphoribosyltransferase | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.193 | - |
guanine | at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
0.89 | - |
hypoxanthine | at pH 7.5 and 25°C | Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.29 | - |
5-phospho-alpha-D-ribose 1-diphosphate | with guanine as cosubstrate, at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
0.63 | - |
5-phospho-alpha-D-ribose 1-diphosphate | with hypoxanthine as cosubstrate, at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
19 | - |
guanine | at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
34 | - |
hypoxanthine | at pH 7.5 and 25°C | Mycobacterium tuberculosis |