Activating Compound | Comment | Organism | Structure |
---|---|---|---|
GTP | activation, stabilization of the tetrameric structure at 2 mM, without GTP enzyme forms dimers | Toxoplasma gondii | |
GTP | about 5-7fold increase of Km for 5-phosphoribose 1-diphosphate, unaltered Vmax | Toxoplasma gondii |
Cloned (Comment) | Organism |
---|---|
functional overexpression in Escherichia coli JM109 | Toxoplasma gondii |
wild-type and mutant C128V | Toxoplasma gondii |
Crystallization (Comment) | Organism |
---|---|
hanging drop-vapour diffusion method, enzyme complexed with uracil and 5-phosphoribose 1-diphosphate, 20 mg/ml, mixing of equal volumes, 15 mM sodium citrate/phosphate, 0.2 M NaCl, pH 4.7, 10% polyethylene glycol 3400, X-ray diffraction structure analysis, dynamic light scattering | Toxoplasma gondii |
Protein Variants | Comment | Organism |
---|---|---|
C128V | site-directed mutagenesis, required for structural sudy | Toxoplasma gondii |
K150A | site-directed mutagenesis, slightly reduced kcat, increased Km for 5-phosphoribose 1-diphosphate in presence of GTP, structural sudy | Toxoplasma gondii |
K59A | site-directed mutagenesis, slightly enhanced kcat, increased Km for 5-phosphoribose 1-diphosphate in presence of GTP, structural sudy | Toxoplasma gondii |
R68A | site-directed mutagenesis, slightly reduced kcat, increased Km for 5-phosphoribose 1-diphosphate in presence of GTP, structural sudy | Toxoplasma gondii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km-values, recombinant mutant enzymes | Toxoplasma gondii | |
0.037 | - |
5-phospho-alpha-D-ribose 1-diphosphate | recombinant wild-type, with GTP | Toxoplasma gondii | |
0.216 | - |
5-phospho-alpha-D-ribose 1-diphosphate | recombinant wild-type, without GTP | Toxoplasma gondii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
uracil + 5-phospho-alpha-D-ribose 1-diphosphate | Toxoplasma gondii | pyrimidine salvage enzyme, no de novo synthesis of pyrmidines | UMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Toxoplasma gondii | Q26998 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UMP + diphosphate = uracil + 5-phospho-alpha-D-ribose 1-diphosphate | sequential reaction mechanism | Toxoplasma gondii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
uracil + 5-phospho-alpha-D-ribose 1-diphosphate | transfer to N1 of uracil | Toxoplasma gondii | UMP + diphosphate | - |
? | |
uracil + 5-phospho-alpha-D-ribose 1-diphosphate | 5-phospho-alpha-D-ribose 1-diphosphate binding site | Toxoplasma gondii | UMP + diphosphate | - |
? | |
uracil + 5-phospho-alpha-D-ribose 1-diphosphate | pyrimidine salvage enzyme, no de novo synthesis of pyrmidines | Toxoplasma gondii | UMP + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | three-dimensional structure | Toxoplasma gondii |
More | a tetramer of 2 dimers, equilibrium between dimeric and tetrameric form | Toxoplasma gondii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | recombinant wild-type and mutant enzymes | Toxoplasma gondii |