Cloned (Comment) | Organism |
---|---|
gene AGOS_AFR052C, sequence comparisons, phylogenetic tree, functional recombinant expression of the enzyme with an N-terminal fusion to TrxA as a soluble enzyme in Escherichia coli | Eremothecium gossypii |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the pyrimidine auxotroph Ashbya gossypii mutant Agura3 shows hypersensitivity to uracil. Uridine is the only intermediate of the pyrimidine salvage pathway able to fully restore this mutant's growth. Overexpression of the AgUPRT encoding-gene in Ashbya gossypii mutant Agura3 leads to similar growth on rich medium containing 5 mM uracil or uridine | Eremothecium gossypii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Uracil | concentrations above 1mM negatively affect UPRT activity | Eremothecium gossypii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UMP + diphosphate | Eremothecium gossypii | - |
uracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
UMP + diphosphate | Eremothecium gossypii CBS 109.51 | - |
uracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
UMP + diphosphate | Eremothecium gossypii FGSC 9923 | - |
uracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
UMP + diphosphate | Eremothecium gossypii ATCC 10895 | - |
uracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
UMP + diphosphate | Eremothecium gossypii NRRL Y-1056 | - |
uracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Eremothecium gossypii | Q754M0 | i.e. Eremothecium gossypii | - |
Eremothecium gossypii ATCC 10895 | Q754M0 | i.e. Eremothecium gossypii | - |
Eremothecium gossypii CBS 109.51 | Q754M0 | i.e. Eremothecium gossypii | - |
Eremothecium gossypii FGSC 9923 | Q754M0 | i.e. Eremothecium gossypii | - |
Eremothecium gossypii NRRL Y-1056 | Q754M0 | i.e. Eremothecium gossypii | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli, cleavage of the TrxA fusion protein with TEV protease | Eremothecium gossypii |
Storage Stability | Organism |
---|---|
4°C, purified detagged recombinant enzyme, in Tris-NaCl buffer, stable for at least 1 week | Eremothecium gossypii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UMP + diphosphate | - |
Eremothecium gossypii | uracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
UMP + diphosphate | maximum activity is obtained with 0.4-0.5 mM uracil in the presence of 1 mM PRPP, decreasing with increasing uracil concentrations thereafter. The activity in the presence of 0.5 mM uracil and at varying concentrations of PRPP is maximal at 0.75-1.0 mM PRPP, remaining constant thereafter | Eremothecium gossypii | uracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
UMP + diphosphate | - |
Eremothecium gossypii CBS 109.51 | uracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
UMP + diphosphate | maximum activity is obtained with 0.4-0.5 mM uracil in the presence of 1 mM PRPP, decreasing with increasing uracil concentrations thereafter. The activity in the presence of 0.5 mM uracil and at varying concentrations of PRPP is maximal at 0.75-1.0 mM PRPP, remaining constant thereafter | Eremothecium gossypii CBS 109.51 | uracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
UMP + diphosphate | - |
Eremothecium gossypii FGSC 9923 | uracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
UMP + diphosphate | maximum activity is obtained with 0.4-0.5 mM uracil in the presence of 1 mM PRPP, decreasing with increasing uracil concentrations thereafter. The activity in the presence of 0.5 mM uracil and at varying concentrations of PRPP is maximal at 0.75-1.0 mM PRPP, remaining constant thereafter | Eremothecium gossypii FGSC 9923 | uracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
UMP + diphosphate | - |
Eremothecium gossypii ATCC 10895 | uracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
UMP + diphosphate | maximum activity is obtained with 0.4-0.5 mM uracil in the presence of 1 mM PRPP, decreasing with increasing uracil concentrations thereafter. The activity in the presence of 0.5 mM uracil and at varying concentrations of PRPP is maximal at 0.75-1.0 mM PRPP, remaining constant thereafter | Eremothecium gossypii ATCC 10895 | uracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
UMP + diphosphate | - |
Eremothecium gossypii NRRL Y-1056 | uracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
UMP + diphosphate | maximum activity is obtained with 0.4-0.5 mM uracil in the presence of 1 mM PRPP, decreasing with increasing uracil concentrations thereafter. The activity in the presence of 0.5 mM uracil and at varying concentrations of PRPP is maximal at 0.75-1.0 mM PRPP, remaining constant thereafter | Eremothecium gossypii NRRL Y-1056 | uracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 26000, recombinant detagged enzyme, SDS-PAGE | Eremothecium gossypii |
Synonyms | Comment | Organism |
---|---|---|
AFR052C | - |
Eremothecium gossypii |
AFR052Cp | UniProt | Eremothecium gossypii |
AGOS_AFR052C | locus name | Eremothecium gossypii |
AgUPRT | - |
Eremothecium gossypii |
UPRT | - |
Eremothecium gossypii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Eremothecium gossypii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Eremothecium gossypii |
General Information | Comment | Organism |
---|---|---|
evolution | UPRT is an enzyme that is highly conserved across species and has been characterized in several organisms, from prokaryotes to human | Eremothecium gossypii |
malfunction | the pyrimidine auxotroph Ashbya gossypii mutant Agura3 shows hypersensitivity to uracil. The mutant's growth can be restored by uridine. In silico analysis of the AgUPRT does not highlight any particular feature that can account for limitations in its enzymatic activity | Eremothecium gossypii |
physiological function | uracil phosphoribosyltransferase (UPRT) is the pyrimidine salvage pathway enzyme responsible for converting uracil to uridine monophosphate in the presence of phosphoribosyl pyrophosphate (PRPP). Uracil-mediated regulation mechanism of the UPRT activity | Eremothecium gossypii |