Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | 5 mM, 31% inhibition | Pumpkin |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0013 | - |
geranyl diphosphate | - |
Pumpkin |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Pumpkin | |
Mn2+ | can partially replace Mg2+, less effective | Pumpkin |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + 2 isopentenyl diphosphate | Pumpkin | enzyme participates in isoprenoid biosynthesis in eukaryotes | (E,E)-farnesyl diphosphate + 2 diphosphate | - |
? | |
isopentenyl diphosphate + geranyl diphosphate | Pumpkin | enzyme participates in isoprenoid biosynthesis in eukaryotes | diphosphate + farnesyl diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pumpkin | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, Sephadex G 200, hydroxylapatite | Pumpkin |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Pumpkin | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + 2 isopentenyl diphosphate | activity is higher than with geranyl diphosphate | Pumpkin | (E,E)-farnesyl diphosphate + 2 diphosphate | - |
? | |
dimethylallyl diphosphate + 2 isopentenyl diphosphate | enzyme participates in isoprenoid biosynthesis in eukaryotes | Pumpkin | (E,E)-farnesyl diphosphate + 2 diphosphate | - |
? | |
farnesyl diphosphate + isopentenyl diphosphate | less than 5% of the activity with dimethylallyl diphosphate | Pumpkin | geranyl diphosphate + diphosphate | - |
? | |
geranyl diphosphate + isopentenyl diphosphate | - |
Pumpkin | diphosphate + farnesyl diphosphate | - |
? | |
isopentenyl diphosphate + geranyl diphosphate | enzyme participates in isoprenoid biosynthesis in eukaryotes | Pumpkin | diphosphate + farnesyl diphosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Pumpkin |