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Literature summary for 2.5.1.10 extracted from
- Structural characterization of substrate and inhibitor binding to farnesyl pyrophosphate synthase from Pseudomonas aeruginosa (2015), Acta Crystallogr. Sect. D, 71, 721-731.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Pseudomonas aeruginosa |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| in complex with natural substrates and inhibitors, sitting drop vapor diffusion method, using 20% (w/v) PEG 3350, 0.2 M NaF, 0.1 M bis-Tris propane pH 6.5 | Pseudomonas aeruginosa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ibandronate | - |
Pseudomonas aeruginosa |  |
| zoledronate | - |
Pseudomonas aeruginosa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | contains Mg2+ | Pseudomonas aeruginosa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34018 | - |
ESI-MS | Pseudomonas aeruginosa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| geranyl diphosphate + isopentenyl diphosphate | Pseudomonas aeruginosa | - |
diphosphate + (2E,6E)-farnesyl diphosphate | - |
? | |
| isopentenyl diphosphate + dimethylallyl diphosphate | Pseudomonas aeruginosa | - |
diphosphate + geranyl diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | Q9HWY4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA resin column chromatography and Superdex 200 gel filtration | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| geranyl diphosphate + isopentenyl diphosphate | - |
Pseudomonas aeruginosa | diphosphate + (2E,6E)-farnesyl diphosphate | - |
? | |
| isopentenyl diphosphate + dimethylallyl diphosphate | - |
Pseudomonas aeruginosa | diphosphate + geranyl diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 34018, calculated from amino acid sequence | Pseudomonas aeruginosa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| farnesyl pyrophosphate synthase | - |
Pseudomonas aeruginosa |
| FPPS | - |
Pseudomonas aeruginosa |
| PA4043 | - |
Pseudomonas aeruginosa |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0027 | - |
at pH 8.0 and 22°C | Pseudomonas aeruginosa | ibandronate | |
| 0.0152 | - |
at pH 8.0 and 22°C | Pseudomonas aeruginosa | zoledronate | |
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