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Literature summary for 2.5.1.111 extracted from

  • Araya-Cloutier, C.; Martens, B.; Schaftenaar, G.; Leipoldt, F.; Gruppen, H.; Vincken, J.
    Structural basis for non-genuine phenolic acceptor substrate specificity of Streptomyces roseochromogenes prenyltransferase CloQ from the ABBA/PT-barrel superfamily (2017), PLoS ONE, 12, e0174665 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.111

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta (DE3) pLysS cells Streptomyces roseochromogenus subsp. oscitans

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 7 mM used in assay conditions Streptomyces roseochromogenus subsp. oscitans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dimethylallyl diphosphate + 4-hydroxyphenylpyruvate Streptomyces roseochromogenus subsp. oscitans
-
 diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces roseochromogenus subsp. oscitans Q8GHB2
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography Streptomyces roseochromogenus subsp. oscitans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate + 3'-hydroxydaidzein 2% conversion Streptomyces roseochromogenus subsp. oscitans ?
-
 ?
dimethylallyl diphosphate + 4'-hydroxy-6-methoxyflavone 1% conversion Streptomyces roseochromogenus subsp. oscitans ?
-
 ?
dimethylallyl diphosphate + 4'-hydroxy-7-methoxyflavone 2% conversion Streptomyces roseochromogenus subsp. oscitans ?
-
 ?
dimethylallyl diphosphate + 4'-hydroxy-7-methoxyflavone 2% conversion Streptomyces roseochromogenus subsp. oscitans diphosphate + 2-[4-hydroxy-3-(3-methylbut-2-en-1-yl)phenyl]-7-methoxy-4H-chromen-4-one + 7-methoxy-2-[4-[(3-methylbut-2-en-1-yl)oxy]phenyl]-4H-chromen-4-one
-
 ?
dimethylallyl diphosphate + 4-hydroxyphenylpyruvate
-
 Streptomyces roseochromogenus subsp. oscitans diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate
-
 ?
dimethylallyl diphosphate + 4-hydroxyphenylpyruvate 84% conversion Streptomyces roseochromogenus subsp. oscitans diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate
-
 ?
dimethylallyl diphosphate + 7,4'-dihydroxyflavone 4% conversion Streptomyces roseochromogenus subsp. oscitans ?
-
 ?
dimethylallyl diphosphate + coumestrol 1% conversion Streptomyces roseochromogenus subsp. oscitans ?
-
 ?
dimethylallyl diphosphate + daidzein less than 1% conversion Streptomyces roseochromogenus subsp. oscitans ?
-
 ?
dimethylallyl diphosphate + equol 8% conversion Streptomyces roseochromogenus subsp. oscitans ?
-
 ?
dimethylallyl diphosphate + equol 2% conversion Streptomyces roseochromogenus subsp. oscitans diphosphate + 3-[4-hydroxy-3-(3-methylbut-2-en-1-yl)phenyl]-3,4-dihydro-2H-chromen-7-ol + 3-(4-hydroxyphenyl)-6-(3-methylbut-2-en-1-yl)-3,4-dihydro-2H-chromen-7-ol
-
 ?
dimethylallyl diphosphate + genistein less than 1% conversion Streptomyces roseochromogenus subsp. oscitans ?
-
 ?
dimethylallyl diphosphate + luteolin 3% conversion Streptomyces roseochromogenus subsp. oscitans ?
-
 ?
dimethylallyl diphosphate + resveratrol 2% conversion Streptomyces roseochromogenus subsp. oscitans ?
-
 ?
additional information the enzyme shows no activity with isoflavone and glycitein Streptomyces roseochromogenus subsp. oscitans ?
-
-

Subunits

Subunits Comment Organism
? x * 35000, SDS-PAGE Streptomyces roseochromogenus subsp. oscitans

Synonyms

Synonyms Comment Organism
CloQ
-
 Streptomyces roseochromogenus subsp. oscitans