Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Leptosphaeria maculans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 5 mM, 12% inhibition | Leptosphaeria maculans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
L-tyrosine | pH 7.5, 37°C | Leptosphaeria maculans | |
0.17 | - |
dimethylallyl diphosphate | pH 7.5, 37°C | Leptosphaeria maculans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 5 mM, 1.4fold activation compared to activity without additives | Leptosphaeria maculans | |
additional information | no requirement for divalent metal ion | Leptosphaeria maculans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
2 * 50000, SDS-PAGE | Leptosphaeria maculans |
100000 | - |
His6-tagged enzyme, gel filtration | Leptosphaeria maculans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + L-tyrosine | Leptosphaeria maculans | the enzyme is involved in biosynthesis of the phytotoxin sirodesmin PL | diphosphate + 4-O-dimethylallyl-L-tyrosine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leptosphaeria maculans | Q6Q874 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Leptosphaeria maculans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + L-3,4-dihydroxyphenylalanine | 16.6% of the activity, compared to L-tyrosine. It is expected that the prenylation takes place at the 4-hydroxyl group. The structure of the product is not determined | Leptosphaeria maculans | ? | - |
? | |
dimethylallyl diphosphate + L-tyrosine | the enzyme is involved in biosynthesis of the phytotoxin sirodesmin PL | Leptosphaeria maculans | diphosphate + 4-O-dimethylallyl-L-tyrosine | - |
? | |
dimethylallyl diphosphate + L-tyrosine | no activity with cyclo-L-Tyr-L-Ser, L-Phe, 4-hydroxybenzoate or 4-coumarate | Leptosphaeria maculans | diphosphate + 4-O-dimethylallyl-L-tyrosine | - |
? | |
additional information | SirD also prenylates L-Trp, resulting in the formation of 7-dimethylallyltryptophan. SirD catalyses the C-prenylation of L-Trp, in addition to the O-prenylation of L-Tyr, cf. EC 2.5.1.34 | Leptosphaeria maculans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 50000, SDS-PAGE | Leptosphaeria maculans |
Synonyms | Comment | Organism |
---|---|---|
SirD | - |
Leptosphaeria maculans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Leptosphaeria maculans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
L-tyrosine | pH 7.5, 37°C | Leptosphaeria maculans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Leptosphaeria maculans |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in biosynthesis of the epidithiopiperazinedione toxin sirodesmin PL by the phytopathogenic ascomycete Leptosphaeria maculans | Leptosphaeria maculans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.7 | - |
L-tyrosine | pH 7.5, 37°C | Leptosphaeria maculans |