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Literature summary for 2.5.1.132 extracted from
- Elimination of 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid 9-phosphate synthase activity from human N-acetylneuraminic acid 9-phosphate synthase by a single mutation (2006), Biochem. J., 397, 195-201.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) Gold cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| M42L | the M42L mutant of N-acetylneuraminic acid-9-phosphate synthase, like the wild type enzyme, shows the additional 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid-9-phosphate synthase activity | Homo sapiens |
| M42T | the mutation of N-acetylneuraminic acid-9-phosphate synthase abolishes its 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid-9-phosphate synthase activity | Homo sapiens |
| R96K/R100S/E103Q/V105I/V195A | the mutant of N-acetylneuraminic acid-9-phosphate synthase has significant 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid-9-phosphate synthase activity | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.5 | 5 | D-mannose 6-phosphate | mutant enzyme M42L, at pH 7.5 and 37°C | Homo sapiens |  |
| 2.62 | - |
D-mannose 6-phosphate | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + D-mannose 6-phosphate + H2O | Homo sapiens | - |
3-deoxy-D-glycero-D-galacto-nononate 9-phosphate + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| nickel affinity column chromatography | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + D-mannose 6-phosphate + H2O | - |
Homo sapiens | 3-deoxy-D-glycero-D-galacto-nononate 9-phosphate + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid 9-phosphate synthase | - |
Homo sapiens |
| Neu5Ac 9-phosphate synthase | shows also -keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid-9-phosphate synthase activity | Homo sapiens |
| neu5ac-9-P synthase | shows also -keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid-9-phosphate synthase activity | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0011 | - |
D-mannose 6-phosphate | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens | |
| 0.0083 | - |
D-mannose 6-phosphate | mutant enzyme M42L, at pH 7.5 and 37°C | Homo sapiens | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000327 | - |
D-mannose 6-phosphate | mutant enzyme M42L, at pH 7.5 and 37°C | Homo sapiens | |
| 0.000426 | - |
D-mannose 6-phosphate | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens | |
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