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Literature summary for 2.5.1.16 extracted from
- Purification and partial characterization of human polyamine synthases (1989), Biochem. J., 259, 879-886.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5'-methylthioadenosine | - |
Bos taurus |  |
| 5'-methylthioadenosine | - |
Homo sapiens | |
| 5'-methylthioadenosine | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.007 | - |
S-adenosyl-3-methylthio-1-propylamine | pH 7.4, 37ºC, spleen | Homo sapiens | |
| 0.08 | - |
1,4-diaminobutane | pH 7.4, 37ºC, spleen | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Homo sapiens | 5829 | - |
| cytosol | - |
Bos taurus | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
2 * 35000, SDS-PAGE | Homo sapiens |
| 62000 | - |
spleen, pore gradient gel electrophoresis | Homo sapiens |
| 62000 | - |
brain, pore gradient gel electrophoresis | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
| affinity chromatography | Bos taurus |
| ammonium sulfate fractionation, chromatography on DEAE-Cellulose, affinity chromatography, 8700fold purified | Homo sapiens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| S-adenosyl 3-(methylsulfanyl)propylamine + putrescine = S-methyl-5'-thioadenosine + spermidine | Michaelis-Menten kinetics | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Homo sapiens | - |
| brain | - |
Rattus norvegicus | - |
| brain | - |
Bos taurus | - |
| kidney | - |
Homo sapiens | - |
| kidney | - |
Rattus norvegicus | - |
| lung | - |
Homo sapiens | - |
| additional information | tissue distribution | Homo sapiens | - |
| additional information | tissue distribution | Rattus norvegicus | - |
| pancreas | - |
Homo sapiens | - |
| pancreas | - |
Rattus norvegicus | - |
| placenta | - |
Homo sapiens | - |
| spleen | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.66 | - |
- |
Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80ºC, 20% glycerol, rapid loss of activity in pancreas preparations | Homo sapiens |
| -80ºC, 20% glycerol, stable for spleen tissue enzyme during storage and freeze-thawings | Homo sapiens |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Rattus norvegicus |
| dimer | - |
Bos taurus |
| dimer | 2 * 35000, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
| 37 | - |
assay at | Rattus norvegicus |
| 37 | - |
assay at | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
| additional information | - |
- |
Bos taurus |
| additional information | - |
pI 5.1, in spleen | Homo sapiens |
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Release 2023.1 (January 2023)
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