Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cyclohexylamine | potent inhibition, reduces the rate of spermidine synthesis by more than 90% at 0.1 mM | Escherichia coli | |
Cyclohexylamine | potent inhibition, reduces the rate of spermidine synthesis by more than 90% at 0.1 mM | Pseudomonas aeruginosa | |
Cyclohexylamine | - |
Rattus norvegicus | |
Cyclohexylamine | potent inhibition, reduces the rate of spermidine synthesis by more than 90% at 0.1 mM | Serratia marcescens | |
S-Adenosyl-1,8-diamino-3-thiooctane | competitive with 1,4-diaminobutane, potent inhibition, stronger than dicyclohexylamine in vitro, but not in vivo, at concentrations of S-adenosyl-3-methylthio-1-propylamine and 1,4-diaminobutane higher than those normally present in vivo. At concentration of substrates that approximate in vivo conditions, more than 20fold stronger inhibition | Escherichia coli | |
S-Adenosyl-1,8-diamino-3-thiooctane | competitive with 1,4-diaminobutane, potent inhibition, stronger than dicyclohexylamine in vitro, but not in vivo, at concentrations of S-adenosyl-3-methylthio-1-propylamine and 1,4-diaminobutane higher than those normally present in vivo. At concentration of substrates that approximate in vivo conditions, more than 20fold stronger inhibition | Pseudomonas aeruginosa | |
S-Adenosyl-1,8-diamino-3-thiooctane | competitive with 1,4-diaminobutane, potent inhibition, stronger than dicyclohexylamine in vitro, but not in vivo, at concentrations of S-adenosyl-3-methylthio-1-propylamine and 1,4-diaminobutane higher than those normally present in vivo. At concentration of substrates that approximate in vivo conditions, more than 20fold stronger inhibition | Rattus norvegicus | |
S-Adenosyl-1,8-diamino-3-thiooctane | competitive with 1,4-diaminobutane, potent inhibition, stronger than dicyclohexylamine in vitro, but not in vivo, at concentrations of S-adenosyl-3-methylthio-1-propylamine and 1,4-diaminobutane higher than those normally present in vivo. At concentration of substrates that approximate in vivo conditions, more than 20fold stronger inhibition | Serratia marcescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
1,4-diaminobutane | pH 7.5, 37ºC | Escherichia coli | |
0.2 | - |
1,4-diaminobutane | - |
Rattus norvegicus | |
0.2 | - |
1,4-diaminobutane | pH 7.5, 37ºC | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane | Escherichia coli | - |
5'-methylthioadenosine + spermidine | - |
? | |
S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane | Rattus norvegicus | - |
5'-methylthioadenosine + spermidine | - |
? | |
S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane | Pseudomonas aeruginosa | - |
5'-methylthioadenosine + spermidine | - |
? | |
S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane | Serratia marcescens | - |
5'-methylthioadenosine + spermidine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Pseudomonas aeruginosa | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Serratia marcescens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ventral prostate | Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl 3-(methylsulfanyl)propylamine + putrescine = S-methyl-5'-thioadenosine + spermidine | ping-pong mechanism with a propylaminated form of the enzyme as an obligatory intermediate | Escherichia coli | |
S-adenosyl 3-(methylsulfanyl)propylamine + putrescine = S-methyl-5'-thioadenosine + spermidine | single displacement reaction | Escherichia coli | |
S-adenosyl 3-(methylsulfanyl)propylamine + putrescine = S-methyl-5'-thioadenosine + spermidine | single displacement reaction | Pseudomonas aeruginosa | |
S-adenosyl 3-(methylsulfanyl)propylamine + putrescine = S-methyl-5'-thioadenosine + spermidine | single displacement reaction | Serratia marcescens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
prostate gland | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane | - |
Escherichia coli | 5'-methylthioadenosine + spermidine | - |
? | |
S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane | - |
Rattus norvegicus | 5'-methylthioadenosine + spermidine | - |
? | |
S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane | - |
Pseudomonas aeruginosa | 5'-methylthioadenosine + spermidine | - |
? | |
S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane | - |
Serratia marcescens | 5'-methylthioadenosine + spermidine | - |
? | |
S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane | - |
Escherichia coli | 5'-methylthioadenosine + spermidine | - |
? | |
S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane | - |
Rattus norvegicus | 5'-methylthioadenosine + spermidine | - |
? | |
S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane | - |
Pseudomonas aeruginosa | 5'-methylthioadenosine + spermidine | - |
? | |
S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane | - |
Serratia marcescens | 5'-methylthioadenosine + spermidine | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
37 | - |
assay at | Rattus norvegicus |
37 | - |
assay at | Pseudomonas aeruginosa |
37 | - |
assay at | Serratia marcescens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000008 | - |
S-Adenosyl-1,8-diamino-3-thiooctane | prostate | Rattus norvegicus | |
0.00005 | - |
S-Adenosyl-1,8-diamino-3-thiooctane | - |
Escherichia coli |